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CHEMR23 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244726

Chemokine-Like Receptor 1 (CMKLR1) is an chemoattractant receptor. CMKLR1 is thought to act primarily in bone development where it is differentially regulated in osseous and cartilaginous tissue. It also acts as a coreceptor for immunodeficiency viruses.… More details

$630 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CHEMR23 / CMKLR1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human CHEMR23 / CMKLR1
Product Gene Name
anti-CHEMR23 antibody
Product Synonym Gene Name
CMKLR1
Product Synonym Names
Anti-CHEMR23 / CMKLR1 Antibody (N-Terminus) IHC-plus; CMKLR1; Chemokine receptor-like 1; DEZ; CHEMERINR; Resolvin E1 receptor; RVER1; Chemokine-like receptor 1; ChemR23; G-protein coupled receptor DEZ; Human CHEMR23; CMKLR1
Clonality
Polyclonal
Host
CHEMR23/CMKR1 antibody was produced in Rabbit
Species Reactivity
Human, Monkey, Dog, Rabbit
Predicted Reactivity: Horse
Form/Format
PBS, 0.1% Sodium Azide
Purity/Purification
Immunoaffinity purified
Specificity
Human CMKLR1, BLAST analysis of the peptide immunogen showed no homology with other human protiens except FZD6 (106%)
Preparation and Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
OMIM
602351
3D Structure
ModBase 3D Structure for Q99788
Target
CHEMR23/CMKLR1
Family
GPCR/Chemoattractant
Immunogen Species
CHEMR23/CMKLR1 antibody was raised against Human
Antigen Type
Synthetic Type
Immunogen
CHEMR23/CMKLR1 antibody was raised against synthetic 18 amino acid peptide from N-Terminus extracellular domain of human CMKLR1. Percent identity with other species by BLAST analysis
Epitope
N-Terminus
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CHEMR23 antibody

Family: GPCR; Subfamily: Chemoattractant
Applications Tested/Suitable for anti-CHEMR23 antibody
Immunohistochemistry, Immunohistochemistry-Paraffin
Application Notes for anti-CHEMR23 antibody
IHC-P: 7ug/ml
(Optimal dilution to be determined by researcher)

Immunohistochemistry (IHC) of anti-CHEMR23 antibody

Anti-CMKLR1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-CHEMR23 Polyclonal Antibody – Cat# MBS244726

Other Notes

Small volumes of anti-CHEMR23 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHEMR23 purchase

MBS244726 is an antibody targeting CHEMR23. Also known as Rabbit Polyclonal to Human CHEMR23 / CMKLR1. Reactive in Human, Monkey, Dog, Rabbit
Predicted Reactivity: Horse. Host: CHEMR23/CMKR1 antibody was produced in Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHEMR23. It may not necessarily be applicable to this product.

NCBI GI #
4758014
NCBI GeneID
1240
NCBI Accession #
NP_004063.1
UniProt Primary Accession #
Q99788
UniProt Secondary Accession #
O75748; Q3KP37; Q5U0H0; Q99789; A8K6Y5
UniProt Related Accession #
Q99788
GenBank Nucleotide #
NM_004072.2
NCBI Official Full Name
chemokine-like receptor 1 isoform b
NCBI Official Synonym Full Names
chemokine-like receptor 1
NCBI Official Symbol
CMKLR1
NCBI Official Synonym Symbols
DEZ; RVER1; ChemR23; CHEMERINR
UniProt Protein Name
Chemokine-like receptor 1
UniProt Synonym Protein Names
G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ
UniProt Gene Name
CMKLR1
UniProt Synonym Gene Names
CHEMR23; DEZ
UniProt Entry Name
CML1_HUMAN
NCBI Protein Information
chemokine-like receptor 1; chemerin receptor; resolvin E1 receptor; chemokine receptor-like 1; G-protein coupled receptor DEZ; G-protein coupled receptor ChemR23; orphan G-protein coupled receptor, Dez

UniProt Comments for CHEMR23

CMKLR1: Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF- kappa-B. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2). Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; protein binding; chemokine receptor activity; receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of interleukin-12 production; inhibition of NF-kappaB transcription factor; positive regulation of fat cell differentiation; regulation of calcium-mediated signaling; immune response; chemotaxis; skeletal development
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Research Articles on CHEMR23

1. This study identified the up-regulation of ChemR23 in post-traumatic injury of calcaneal articular fracture

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHEMR23 antibody

 Reference Product  PubMed Publications
 RARRES2 antibody  >4 publications with CHEMR23 and RARRES2

Pathways associated with anti-CHEMR23 antibody

 Products by Pathway  Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram

Diseases associated with anti-CHEMR23 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >54 publications with CHEMR23 and Inflammation
 Necrosis Antibodies  >13 publications with CHEMR23 and Necrosis
 Insulin Resistance Antibodies  >11 publications with CHEMR23 and Insulin Resistance
 Lung Diseases Antibodies  >8 publications with CHEMR23 and Lung Diseases
 Liver Diseases Antibodies  >8 publications with CHEMR23 and Liver Diseases
 Cardiovascular Diseases Antibodies  >7 publications with CHEMR23 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >6 publications with CHEMR23 and Disease Models, Animal
 Pain Antibodies  >5 publications with CHEMR23 and Pain
 Fatty Liver Antibodies  >5 publications with CHEMR23 and Fatty Liver
 Nervous System Diseases Antibodies  >3 publications with CHEMR23 and Nervous System Diseases

Organs/Tissues associated with anti-CHEMR23 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >50 publications with CHEMR23 and Blood
 Adipose Tissue Antibodies  >25 publications with CHEMR23 and Adipose Tissue
 Connective Tissue Antibodies  >15 publications with CHEMR23 and Connective Tissue
 Bone Antibodies  >14 publications with CHEMR23 and Bone
 Lung Antibodies  >11 publications with CHEMR23 and Lung
 Heart Antibodies  >7 publications with CHEMR23 and Heart
 Spleen Antibodies  >4 publications with CHEMR23 and Spleen
 Brain Antibodies  >4 publications with CHEMR23 and Brain
 Testis Antibodies  >2 publications with CHEMR23 and Testis
 Kidney Antibodies  >2 publications with CHEMR23 and Kidney
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