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CHCHD3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244852

MBS244852 is an antibody targeting CHCHD3. Also known as Goat Polyclonal to Human CHCHD3. Reactive in Gibbon, HumanPredicted Reactivity: Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CHCHD3, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human CHCHD3
Product Gene Name
Product Synonym Names
Anti-CHCHD3 Antibody (Internal) IHC-plus; CHCHD3; PPP1R22; MINOS3; Human CHCHD3
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Human
Predicted Reactivity: Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human CHCHD3.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
26,152 Da
OMIM
613748
3D Structure
ModBase 3D Structure for Q9NX63
Target Species
Human
Immunogen Description
Synthetic peptide C-RSSEFYRVTTEQYQ from an internal region of human CHCHD3 (NP_060282.1). Percent identity by BLAST analysis: Human, Gibbon (100%); Monkey, Sheep, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (93%).
Immunogen Type
Synthetic peptide
Immunogen
CHCHD3 antibody was raised against synthetic peptide C-RSSEFYRVTTEQYQ from an internal region of human CHCHD3 (NP_060282.1). Percent identity by BLAST analysis: Human, Gibbon (100%); Monkey, Sheep, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (93%); Marmoset, Mouse, Rat, Hamster, Opossum (86%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-CHCHD3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CHCHD3 antibody
ELISA (1:32000), IHC-P (5 ug/ml), WB (0.3 - 1 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 26.2kDa band observed in Human Placenta lysates (calculated MW of 26kDa according to NP_060282.1). Recommended concentration: 0.3-1 ug/ml.

Immunohistochemistry (IHC) of anti-CHCHD3 antibody

Anti-CHCHD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-CHCHD3 Polyclonal Antibody – Cat# MBS244852

Western Blot (WB) of anti-CHCHD3 antibody

CHCHD3 antibody (0.3 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-CHCHD3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS244852

Other Notes

Small volumes of anti-CHCHD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CHCHD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
MICOS complex subunit MIC19
NCBI Official Synonym Full Names
coiled-coil-helix-coiled-coil-helix domain containing 3
NCBI Official Symbol
CHCHD3
NCBI Official Synonym Symbols
MINOS3; PPP1R22
UniProt Protein Name
MICOS complex subunit MIC19
UniProt Synonym Protein Names
Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
UniProt Gene Name
CHCHD3
UniProt Synonym Gene Names
MIC19; MINOS3
UniProt Entry Name
MIC19_HUMAN
NCBI Protein Information
MICOS complex subunit MIC19; protein phosphatase 1, regulatory subunit 22; mitochondrial inner membrane organizing system 3; coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial

UniProt Comments for CHCHD3

CHCHD3: May be a scaffolding protein that stabilizes protein complexes involved in maintaining mitochondrial crista architecture and protein import.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: mitochondrion; mitochondrial inner membrane; cytoplasm; nucleus

Molecular Function: protein binding; protein complex scaffold; phosphatase binding

Biological Process: inner mitochondrial membrane organization and biogenesis; mitochondrial fusion; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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