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WDHD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245045

MBS245045 is an antibody targeting WDHD1. Also known as Mouse Monoclonal [clone 20G10] (IgG2a) to Human WDHD1. Reactive in Human, Mouse. Host: Mouse. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
WDHD1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 20G10] (IgG2a) to Human WDHD1
Product Gene Name
Product Synonym Names
Anti-WDHD1 Antibody (C-Terminus, clone 20G10) IHC-plus; WDHD1; AND-1; CTF4; AND1; CHTF4; Human WDHD1
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
20G10
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide. Sourced in TCS.
Purity/Purification
Affinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Recombinant (partial), C-terminal
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Molecular Weight
112,705 Da
OMIM
608126
3D Structure
ModBase 3D Structure for O75717
Target Species
Human
Immunogen Description
Recombinant human WDHD1.
Immunogen Type
Recombinant protein
Immunogen
WDHD1 antibody was raised against recombinant human WDHD1.
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-WDHD1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-WDHD1 antibody
IHC-P (15 ug/ml), WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-WDHD1 antibody

Anti-WDHD1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Mouse Anti-WDHD1 Monoclonal Antibody – Cat# MBS245045

Immunohistochemistry (IHC) of anti-WDHD1 antibody

Anti-WDHD1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Mouse Anti-WDHD1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245045

Western Blot (WB) of anti-WDHD1 antibody

Jurkat nuclear cell extracts (lane 1) and NIH3T3 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-AND-1 antibody (clone 20G10). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
Mouse Anti-WDHD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245045

Other Notes

Small volumes of anti-WDHD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WDHD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
WD repeat and HMG-box DNA-binding protein 1 isoform 1
NCBI Official Synonym Full Names
WD repeat and HMG-box DNA binding protein 1
NCBI Official Symbol
WDHD1
NCBI Official Synonym Symbols
AND1; CTF4; AND-1; CHTF4
UniProt Protein Name
WD repeat and HMG-box DNA-binding protein 1
UniProt Synonym Protein Names
Acidic nucleoplasmic DNA-binding protein 1; And-1
UniProt Gene Name
WDHD1
UniProt Synonym Gene Names
AND1; And-1
UniProt Entry Name
WDHD1_HUMAN
NCBI Protein Information
WD repeat and HMG-box DNA-binding protein 1; Acidic nucleoplasmic DNA-binding protein 1; CTF4, chromosome transmission fidelity factor 4 homolog

NCBI Summary for WDHD1

The protein encoded by this gene contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for WDHD1

WDHD1: Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 14q22.2

Cellular Component: nucleoplasm; cytoplasm; chromosome, pericentric region

Molecular Function: protein binding; DNA binding; RNA binding; chromatin binding

Biological Process: RNA processing; regulation of chromosome organization and biogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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