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RPA3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245085

MBS245085 is an antibody targeting RPA3. Also known as Mouse Monoclonal [clone 111] (IgG1) to Human RPA3. Reactive in Human, Mouse. Host: Mouse. More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RPA3, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 111] (IgG1) to Human RPA3
Product Gene Name
anti-RPA3 antibody
Product Synonym Names
RPA3, REPA3, Replication factor A protein 3, Replication protein A3 (14kD), Replication protein A3, 14kDa, RF-A protein 3, RP-A p14, RPA14
Clonality
Monoclonal
Isotype
IgG1
Clone Number
111
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
PBS
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Full-length human RPA-14 expressed in E Coli.
Preparation and Storage
Long term: -20°C; Short term: 4°C.
Avoid repeat freeze-thaw cycles.
OMIM
179837
3D Structure
ModBase 3D Structure for P35244
Antigen Type
Recombinant protein
Immunogen
RPA3 antibody was raised against full-length human RPA-14 expressed in E. coli.
Immunogen Species
RPA3 antibody was raised againist Human.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-RPA3 antibody
Immunohistochemistry (IHC), Immunohistochemistry Paraffin (IHC-P), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-RPA3 antibody
IHC-P: 5 µg/ml
IHC: MBS245085 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245085 was determined to be 5 ug/ml.

(Optimal dilution to be determined by the researcher)

Other Notes

Small volumes of anti-RPA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RPA3. It may not necessarily be applicable to this product.

NCBI GI #
4506587
NCBI GeneID
6119
NCBI Accession #
NP_002938.1
UniProt Primary Accession #
P35244
UniProt Secondary Accession #
Q549U6
UniProt Related Accession #
P35244
GenBank Nucleotide #
NM_002947.3
NCBI Official Full Name
replication protein A 14 kDa subunit
NCBI Official Synonym Full Names
replication protein A3, 14kDa
NCBI Official Symbol
RPA3
NCBI Official Synonym Symbols
REPA3
UniProt Protein Name
Replication protein A 14 kDa subunit
UniProt Synonym Protein Names
Replication factor A protein 3; RF-A protein 3
Protein Family
Replication protein
UniProt Gene Name
RPA3
UniProt Synonym Gene Names
REPA3; RPA14; RP-A p14; RF-A protein 3
UniProt Entry Name
RFA3_HUMAN
NCBI Protein Information
replication protein A 14 kDa subunit; RP-A p14; RF-A protein 3; replication factor A protein 3

UniProt Comments for RPA3

RPA3: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: nucleoplasm; DNA replication factor A complex

Molecular Function: protein binding; damaged DNA binding; single-stranded DNA binding

Biological Process: mismatch repair; regulation of mitotic cell cycle; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; regulation of cell proliferation; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle
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Research Articles on RPA3

1. The allele "T" of rs6947203 in the RPA3 gene acts as a protective allele in glioma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPA3 antibody

 Reference Product  PubMed Publications
 RPA2 antibody  >38 publications with RPA3 and RPA2
 RPA1 antibody  >28 publications with RPA3 and RPA1
 RPA4 antibody  >6 publications with RPA3 and RPA4
 PCNA antibody  >3 publications with RPA3 and PCNA
 MCM4 antibody  >1 publications with RPA3 and MCM4

Pathways associated with anti-RPA3 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway antibodies  Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cellular Response To Heat Stress Pathway antibodies  Cellular Response To Heat Stress Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram

Diseases associated with anti-RPA3 antibody

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >2 publications with RPA3 and Brain Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with RPA3 and Cell Transformation, Neoplastic
 Neoplasm Metastasis Antibodies  >1 publications with RPA3 and Neoplasm Metastasis
 Precancerous Conditions Antibodies  >1 publications with RPA3 and Precancerous Conditions
 Breast Neoplasms Antibodies  >1 publications with RPA3 and Breast Neoplasms
 Weight Loss Antibodies  >1 publications with RPA3 and Weight Loss
 Kidney Diseases Antibodies  >1 publications with RPA3 and Kidney Diseases

Organs/Tissues associated with anti-RPA3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with RPA3 and Brain
 Blood Antibodies  >3 publications with RPA3 and Blood
 Muscle Antibodies  >2 publications with RPA3 and Muscle
 Heart Antibodies  >2 publications with RPA3 and Heart
 Lymph Antibodies  >1 publications with RPA3 and Lymph
 Skin Antibodies  >1 publications with RPA3 and Skin
 Lymph Node Antibodies  >1 publications with RPA3 and Lymph Node
 Kidney Antibodies  >1 publications with RPA3 and Kidney
 Lung Antibodies  >1 publications with RPA3 and Lung
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