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TPX2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245141

TPX2 is a spindle assembly factor, required for normal assembly of mitotic spindles, for normal assembly of microtubules during apoptosis, and for chromatin and/or kinetochore dependent microtubule nucleation. TPX2 mediates AURKA localization to spindle… More details

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Product Overview

Online Datasheet in PDF
Product Name
TPX2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 18D5] (IgG1) to Human TPX2
Product Gene Name
Product Synonym Names
Anti-TPX2 Antibody (clone 18D5) IHC-plus; TPX2; C20orf2; DIL-2; DIL2; HCA519; GD:C20orf1; p100; Protein fls353; Targeting protein for Xklp2; C20orf1; FLS353; HCTP4; REPP86; Human TPX2
Clonality
Monoclonal
Isotype
IgG1
Clone Number
18D5
Host
Mouse
Species Reactivity
Human
Form/Format
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Amino Acid: 1-220 of human TPX2
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
89,393 Da
OMIM
605917
3D Structure
ModBase 3D Structure for Q9ULW0
Target Species
Human
Immunogen Description
Recombinant human TPX2.
Immunogen Type
Recombinant protein
Immunogen
TPX2 antibody was raised against recombinant human TPX2.
Applications Tested/Suitable for anti-TPX2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-TPX2 antibody
IHC-P (10 ug/ml), WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-TPX2 antibody

Anti-TPX2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-TPX2 Monoclonal Antibody – Cat# MBS245141

Western Blot (WB) of anti-TPX2 antibody

MOLT-4 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TPX2 (clone 18D5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Mouse Anti-TPX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245141

Other Notes

Small volumes of anti-TPX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPX2 purchase

MBS245141 is an antibody targeting TPX2. Also known as Mouse Monoclonal [clone 18D5] (IgG1) to Human TPX2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TPX2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
targeting protein for Xklp2
NCBI Official Synonym Full Names
TPX2, microtubule-associated
NCBI Official Symbol
TPX2
NCBI Official Synonym Symbols
DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
UniProt Protein Name
Targeting protein for Xklp2
UniProt Synonym Protein Names
Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; Protein fls353; Restricted expression proliferation-associated protein 100; p100
Protein Family
UniProt Gene Name
TPX2
UniProt Synonym Gene Names
C20orf1; C20orf2; DIL2; HCA519; DIL-2; p100
UniProt Entry Name
TPX2_HUMAN
NCBI Protein Information
targeting protein for Xklp2; protein fls353; TPX2, microtubule-associated, homolog; differentially expressed in lung cells; TPX2, microtubule-associated protein homolog; preferentially expressed in colorectal cancer; hepatocellular carcinoma-associated antigen 519; restricted expression proliferation associated protein 100; restricted expression proliferation-associated protein 100; differentially expressed in cancerous and non-cancerous lung cells 2

UniProt Comments for TPX2

TPX2: a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle.

Protein type: Cell cycle regulation; Microtubule-binding

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: axon hillock; nucleoplasm; microtubule cytoskeleton; spindle pole; microtubule; cytoplasm; spindle; nucleus

Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding

Biological Process: cell proliferation; mitosis; apoptosis; cell division; activation of protein kinase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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