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SSEA-1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245171

Also known as CD15, Lewis x, and 3-fucosyl-N-acetyl-lactosamine. It is synthezised by Fucosyltransferase 4 (FUT4). More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SSEA-1 / Lewis x / CD15, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone HI98] (IgM) to Human SSEA-1 / Lewis x / CD15
Product Gene Name
Product Synonym Names
Anti-SSEA-1 / Lewis x / CD15 Antibody (clone HI98) IHC-plus; SSEA-1/Lewis X/CD15; Human SSEA-1; Lewis x; CD15
Clonality
Monoclonal
Isotype
IgM
Clone Number
HI98
Host
Mouse
Species Reactivity
Chimpanzee, Human
Form/Format
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X.
Preparation and Storage
Store undiluted at 4 degrees C
Molecular Weight
59,084 Da
OMIM
104230
Target Species
Human
Immunogen Description
Human PBMCs were used as immunogen.
Immunogen Type
Cells
Immunogen
Human PBMCs were used as immunogen.
Applications Tested/Suitable for anti-SSEA-1 antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Flow Cytometry (FC/FACS)
Application Notes for anti-SSEA-1 antibody
Flo, IHC-Fr, IHC-P (1:50)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-SSEA-1 antibody

Anti-CD15 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Mouse Anti-SSEA-1 Monoclonal Antibody – Cat# MBS245171

Flow Cytometry (FC/FACS) of anti-SSEA-1 antibody

Human peripheral blood granulocytes stained with purified HI98, followed by anti-mouse IgGs FITC
Mouse Anti-SSEA-1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS245171

Other Notes

Small volumes of anti-SSEA-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SSEA-1 purchase

MBS245171 is an antibody targeting SSEA-1. Also known as Mouse Monoclonal [clone HI98] (IgM) to Human SSEA-1 / Lewis x / CD15. Reactive in Chimpanzee, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SSEA-1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-(1,3)-fucosyltransferase 4
NCBI Official Synonym Full Names
fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
NCBI Official Symbol
FUT4
NCBI Official Synonym Symbols
LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
UniProt Protein Name
Alpha-(1,3)-fucosyltransferase 4
UniProt Synonym Protein Names
ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase
UniProt Gene Name
FUT4
UniProt Synonym Gene Names
ELFT; FCT3A; Fuc-TIV; FucT-IV
UniProt Entry Name
FUT4_HUMAN
NCBI Protein Information
alpha-(1,3)-fucosyltransferase 4; alpha-(1,3)-fucosyltransferase 4; Lewis X; fucT-IV; fucosyltransferase IV; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; galactoside 3-L-fucosyltransferase; stage-specific embryonic antigen 1

NCBI Summary for SSEA-1

The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]

UniProt Comments for SSEA-1

FUT4: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. Belongs to the glycosyltransferase 10 family.

Protein type: Membrane protein, integral; Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.-

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: Golgi apparatus; cell surface; membrane; integral to membrane

Molecular Function: alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity

Biological Process: carbohydrate metabolic process; protein amino acid glycosylation; L-fucose catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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