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TRDMT1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245196

MBS245196 is an antibody targeting TRDMT1. Also known as Mouse Monoclonal [clone 102B1259.2] (IgG) to Mouse TRDMT1 / DNMT2. Reactive in Human, Mouse. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TRDMT1 / DNMT2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 102B1259.2] (IgG) to Mouse TRDMT1 / DNMT2
Product Gene Name
Product Synonym Gene Name
DNMT2
Product Synonym Names
Anti-TRDMT1 / DNMT2 Antibody (clone 102B1259.2) IHC-plus; TRDMT1; DMNT2; DNA MTase homolog HsaIIP; DNMT2; M.HsaIIP; MHSAIIP; RNMT1; DNA methyltransferase-2; PUMET; Mouse TRDMT1; DNMT2
Clonality
Monoclonal
Isotype
IgG
Clone Number
102B1259.2
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
PBS, 0.2% gelatin, 0.05% sodium azide.
Purity/Purification
Protein G Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Synthetic peptides corresponding to mouse Dnmt2.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
12,087 Da
OMIM
602478
3D Structure
ModBase 3D Structure for O14717
Target Species
Mouse
Immunogen Description
Synthetic peptides corresponding to mouse Dnmt2.
Immunogen Type
Synthetic peptide
Immunogen
TRDMT1 / DNMT2 antibody was raised against synthetic peptides corresponding to mouse Dnmt2.
Applications Tested/Suitable for anti-TRDMT1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-TRDMT1 antibody
IHC-P (5 ug/ml), WB (1 - 3 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-TRDMT1 antibody

Anti-TRDMT1 / DNMT2 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-TRDMT1 Monoclonal Antibody – Cat# MBS245196

Western Blot (WB) of anti-TRDMT1 antibody

Western blot analysis for Dnmt2 using antibody at 2 µg/ml dilution against 15 µg of transfected cell lysate.
Mouse Anti-TRDMT1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245196

Other Notes

Small volumes of anti-TRDMT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TRDMT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tRNA (cytosine(38)-C(5))-methyltransferase
NCBI Official Synonym Full Names
tRNA aspartic acid methyltransferase 1
NCBI Official Symbol
TRDMT1
NCBI Official Synonym Symbols
DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
UniProt Protein Name
tRNA (cytosine(38)-C(5))-methyltransferase
UniProt Synonym Protein Names
DNA (cytosine-5)-methyltransferase-like protein 2; Dnmt2; DNA methyltransferase homolog HsaIIP; DNA MTase homolog HsaIIP; M.HsaIIP; PuMet
Protein Family
UniProt Gene Name
TRDMT1
UniProt Synonym Gene Names
DNMT2; Dnmt2; DNA MTase homolog HsaIIP; M.HsaIIP
UniProt Entry Name
TRDMT_HUMAN
NCBI Protein Information
tRNA (cytosine(38)-C(5))-methyltransferase; tRNA (cytosine(38)-C(5))-methyltransferase; DNA methyltransferase-2; DNA MTase homolog HsaIIP; tRNA (cytosine-5-)-methyltransferase; DNA (cytosine-5)-methyltransferase-like protein 2

NCBI Summary for TRDMT1

This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]
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UniProt Comments for TRDMT1

TRDMT1: Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). Belongs to the C5-methyltransferase family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.204; Methyltransferase, tRNA; Amino Acid Metabolism - cysteine and methionine; Methyltransferase

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; DNA binding; RNA binding; tRNA methyltransferase activity

Biological Process: response to amphetamine; tRNA methylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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