Mouse Anti-MCL1 Monoclonal Antibody – Cat# MBS245278

Anti-MCL1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

MCL1 Antibody – Mouse Monoclonal

Name: MCL1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS245278
Price: 625 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245278

Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MCL1 / MCL 1, Monoclonal Antibody

Full Product Name

Mouse Monoclonal [clone 8C6D4B1] (IgG1) to Human MCL1 / MCL 1

Product Gene Name

anti-MCL1 antibody

Product Synonym Gene Name

MCL 1

Product Synonym Names

Anti-MCL1 / MCL 1 Antibody (clone 8C6D4B1) IHC-plus; MCL1; BCL2L3; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; Bcl2-L-3; MCL1S; MCL1L; Myeloid cell leukemia ES; MCL 1; MCL1-ES; TM; EAT; Mcl-1; Human MCL1; MCL 1

Clonality

Monoclonal

Isotype

IgG1

Clone Number

8C6D4B1

Host

Mouse

Species Reactivity

Human

Purity/Purification

Protein G Purified

Concentration

1 mg/ml (lot specific)

Specificity

Ni-NTA purified recombinant protein MCL-1 expressed in E Coli strain M15

Preparation and Storage

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Molecular Weight

28,662 Da

OMIM

159552

3D Structure

ModBase 3D Structure for Q07820

Target Species

Human

Immunogen Description

Recombinant human MCL1.

Immunogen Type

Recombinant protein

Immunogen

MCL1 / MCL 1 antibody was raised against recombinant human MCL1.

Applications Tested/Suitable for anti-MCL1 antibody

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

Application Notes for anti-MCL1 antibody

ELISA (1:10000), IHC-P (10 ug/ml), WB (1:200 - 1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Other Notes

Small volumes of anti-MCL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCL1 purchase

MBS245278 is an antibody targeting MCL1. Also known as Mouse Monoclonal [clone 8C6D4B1] (IgG1) to Human MCL1 / MCL 1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MCL1. It may not necessarily be applicable to this product.

NCBI GI #

11386165

NCBI GeneID

4170

NCBI Accession #

NP_068779.1

UniProt Primary Accession #

Q07820

UniProt Secondary Accession #

Q9HD91; Q9NRQ3; Q9NRQ4; Q9UHR7; Q9UHR8; Q9UHR9; Q9UNJ1; B2R6B2; D3DV03; D3DV04

UniProt Related Accession #

Q07820

GenBank Nucleotide #

NM_021960.4

NCBI Official Full Name

induced myeloid leukemia cell differentiation protein Mcl-1 isoform 1

NCBI Official Synonym Full Names

myeloid cell leukemia 1

NCBI Official Symbol

MCL1

NCBI Official Synonym Symbols

TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

UniProt Protein Name

Induced myeloid leukemia cell differentiation protein Mcl-1

UniProt Synonym Protein Names

Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT

Protein Family

Induced myeloid leukemia cell differentiation protein

UniProt Gene Name

MCL1

UniProt Synonym Gene Names

BCL2L3; Bcl2-L-3

UniProt Entry Name

MCL1_HUMAN

NCBI Protein Information

induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; myeloid cell leukemia ES; bcl-2-related protein EAT/mcl1; myeloid cell leukemia sequence 1 (BCL2-related)

NCBI Summary for MCL1

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

UniProt Comments for MCL1

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Membrane protein, integral; Apoptosis; Inhibitor; Channel, misc.; Mitochondrial

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; multicellular organismal development; response to cytokine stimulus; cellular homeostasis; cell fate determination

Research Articles on MCL1

1. Triptolide has profound antitumor effect on CCA, probably by inducing apoptosis through inhibition of Mcl-1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MCL1 antibody

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
E2F Transcription Factor Network Pathway antibodies	E2F Transcription Factor Network Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
IL-7 Signaling Pathway antibodies	IL-7 Signaling Pathway Diagram
IL6-mediated Signaling Events Pathway antibodies	IL6-mediated Signaling Events Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram

Diseases associated with anti-MCL1 antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>54 publications with MCL1 and Adenocarcinoma
Lung Neoplasms Antibodies	>51 publications with MCL1 and Lung Neoplasms
Necrosis Antibodies	>45 publications with MCL1 and Necrosis
Breast Neoplasms Antibodies	>36 publications with MCL1 and Breast Neoplasms
Liver Diseases Antibodies	>36 publications with MCL1 and Liver Diseases
Liver Neoplasms Antibodies	>29 publications with MCL1 and Liver Neoplasms
Multiple Myeloma Antibodies	>28 publications with MCL1 and Multiple Myeloma
Neoplasms, Experimental Antibodies	>27 publications with MCL1 and Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>27 publications with MCL1 and Carcinoma, Hepatocellular
Inflammation Antibodies	>25 publications with MCL1 and Inflammation

Organs/Tissues associated with anti-MCL1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>147 publications with MCL1 and Blood
Lung Antibodies	>76 publications with MCL1 and Lung
Liver Antibodies	>67 publications with MCL1 and Liver
Bone Antibodies	>58 publications with MCL1 and Bone
Bone Marrow Antibodies	>43 publications with MCL1 and Bone Marrow
Brain Antibodies	>37 publications with MCL1 and Brain
Prostate Antibodies	>30 publications with MCL1 and Prostate
Vascular Antibodies	>22 publications with MCL1 and Vascular
Skin Antibodies	>19 publications with MCL1 and Skin
Lymph Node Antibodies	>13 publications with MCL1 and Lymph Node