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PAG1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245322

MBS245322 is an antibody targeting PAG1. Also known as Mouse Monoclonal [clone MEM-255] (IgG2a) to Human PAG1 / PAG. Reactive in Human. Host: Mouse. More details

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Product Overview

Product Name
PAG1 / PAG, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone MEM-255] (IgG2a) to Human PAG1 / PAG
Product Gene Name
Product Synonym Gene Name
PAG
Product Synonym Names
Anti-PAG1 / PAG Antibody (aa97-432, clone MEM-255) IHC-plus; PAG1; Csk-binding protein; CBP; PAG; Human PAG1; PAG
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
MEM-255
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, 15 mM sodium azide, approx., pH 7.4.
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (lot specific)
Specificity
Recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG)
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Molecular Weight
46,981 Da
OMIM
605767
3D Structure
ModBase 3D Structure for Q9NWQ8
Target Species
Human
Non Reactive Species
Bovine, Mouse, Rat
Immunogen Description
Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).
Immunogen Type
Recombinant protein
Immunogen
PAG1 / PAG antibody was raised against recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).
Antigen Modification
aa97-432
Applications Tested/Suitable for anti-PAG1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-PAG1 antibody
Flo (2 ug/ml), IHC-P (10 ug/ml), WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-PAG1 antibody

Anti-PAG1 / PAG antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-PAG1 Monoclonal Antibody – Cat# MBS245322

Other Notes

Small volumes of anti-PAG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PAG1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphoprotein associated with glycosphingolipid-enriched microdomains 1
NCBI Official Synonym Full Names
phosphoprotein membrane anchor with glycosphingolipid microdomains 1
NCBI Official Symbol
PAG1
NCBI Official Synonym Symbols
CBP; PAG
UniProt Protein Name
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
UniProt Synonym Protein Names
Csk-binding protein; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp
UniProt Gene Name
PAG1
UniProt Synonym Gene Names
CBP; PAG
UniProt Entry Name
PHAG1_HUMAN
NCBI Protein Information
phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Csk-binding protein; transmembrane phosphoprotein Cbp; transmembrane adapter protein PAG; transmembrane adaptor protein PAG; phosphoprotein associated with glycosphingolipid microdomains 1

NCBI Summary for PAG1

The protein encoded by this gene is a type III transmembrane adaptor protein that binds to the tyrosine kinase csk protein. It is thought to be involved in the regulation of T cell activation. [provided by RefSeq, Jul 2008]

UniProt Comments for PAG1

PAG: an ubiquitous type III transmembrane adaptor protein that binds to the tyrosine kinase CSK protein. It is thought to be involved in the regulation of T cell activation.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q21.13

Cellular Component: integral to membrane; plasma membrane; lipid raft

Molecular Function: protein binding; SH3/SH2 adaptor activity; SH2 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of signal transduction; signal transduction; negative regulation of T cell activation; T cell receptor signaling pathway; regulation of T cell activation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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