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STEAP1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245349

MBS245349 is an antibody targeting STEAP1. Also known as Mouse Monoclonal [clone J2D2] (IgG2b,k) to Human STEAP1 / STEAP. Reactive in Human. Host: Mouse. More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
STEAP1 / STEAP, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone J2D2] (IgG2b,k) to Human STEAP1 / STEAP
Product Gene Name
Product Synonym Gene Name
STEAP
Product Synonym Names
Anti-STEAP1 / STEAP Antibody (aa1-70, clone J2D2) IHC-plus; STEAP1; Metalloreductase STEAP1; PRSS24; STEAP; Human STEAP1; STEAP
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
J2D2
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Molecular Weight
39,851 Da
OMIM
604415
3D Structure
ModBase 3D Structure for Q9UHE8
Target Species
Human
Immunogen Description
Recombinant human STEAP1 (1-70aa) purified from E Coli. NCBI Accession No: NP_036581.
Immunogen Type
Recombinant protein
Immunogen
STEAP1 / STEAP antibody was raised against recombinant human STEAP1 (1-70aa) purified from E Coli. NCBI Accession No: NP_036581.
Antigen Modification
aa1-70
Applications Tested/Suitable for anti-STEAP1 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-STEAP1 antibody
ELISA, ICC, IF, IHC-P (10 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-STEAP1 antibody

Anti-STEAP1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-STEAP1 Monoclonal Antibody – Cat# MBS245349

Immunohistochemistry (IHC) of anti-STEAP1 antibody

Anti-STEAP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-STEAP1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245349

Immunofluorescence (IF) of anti-STEAP1 antibody

Immunofluorescence staining of human LNCap cell colony with monoclonal antihuman STEAP1 antibody
Mouse Anti-STEAP1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS245349

Western Blot (WB) of anti-STEAP1 antibody

 
Mouse Anti-STEAP1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245349

Other Notes

Small volumes of anti-STEAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for STEAP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
metalloreductase STEAP1
NCBI Official Synonym Full Names
six transmembrane epithelial antigen of the prostate 1
NCBI Official Symbol
STEAP1
NCBI Official Synonym Symbols
STEAP; PRSS24
UniProt Protein Name
Metalloreductase STEAP1
UniProt Synonym Protein Names
Six-transmembrane epithelial antigen of prostate 1
Protein Family
UniProt Gene Name
STEAP1
UniProt Synonym Gene Names
PRSS24; STEAP
UniProt Entry Name
STEA1_HUMAN
NCBI Protein Information
metalloreductase STEAP1; six-transmembrane epithelial antigen of prostate 1

NCBI Summary for STEAP1

This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. [provided by RefSeq, Jul 2008]
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UniProt Comments for STEAP1

STEAP1: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Belongs to the STEAP family.

Protein type: Cell adhesion; Membrane protein, multi-pass; Oxidoreductase; Membrane protein, integral; EC 1.16.1.-

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: membrane; integral to plasma membrane; plasma membrane; endosome membrane; intercellular junction

Molecular Function: channel activity; transporter activity; metal ion binding; oxidoreductase activity

Biological Process: iron ion homeostasis; ion transport; transmembrane transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-STEAP1 antibody

Pathways associated with anti-STEAP1 antibody

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