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SORL1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245352

MBS245352 is an antibody targeting SORL1. Also known as Mouse Monoclonal (IgG1) to Human SORL1. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
SORL1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal (IgG1) to Human SORL1
Product Gene Name
anti-SORL1 antibody
Product Synonym Names
Anti-SORL1 Antibody IHC-plus; SORL1; C11orf32; LR11; LRP9; gp250; Mosaic protein LR11; SorLA-1; SORLA; Sortilin-related receptor; Human SORL1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3B6B11
Host
Mouse
Species Reactivity
Human
Form/Format
Ascitic fluid, 0.03% sodium azide.
Purity/Purification
Ascites
Specificity
Human
Preparation and Storage
Short term: store at 4°C. Long term: store at -20°C.
OMIM
phenotype 602005
3D Structure
ModBase 3D Structure for Q92673
Immunogen Species
Human
Immunogen
SORL1 antibody was raised against purified recombinant fragment of human SORL1 expressed in E.coli.
Epitope
aa2158-2214
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-SORL1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SORL1 antibody
IHC - Paraffin (1:200)
Immunofluorescence (1:200 - 1:1000)
Western blot (1:100 - 1:500)
ELISA (1:10000)

Immunohistochemistry: MBS245352 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245352 was determined to be 1:200.

Immunohistochemistry (IHC) of anti-SORL1 antibody

Anti-SORL1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Mouse Anti-SORL1 Monoclonal Antibody – Cat# MBS245352

Western Blot (WB) of anti-SORL1 antibody

 
Mouse Anti-SORL1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245352

Other Notes

Small volumes of anti-SORL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SORL1. It may not necessarily be applicable to this product.

NCBI GI #
4507157
NCBI GeneID
6653
NCBI Accession #
NP_003096.1
UniProt Primary Accession #
Q92673
UniProt Secondary Accession #
Q92856; B2RNX7
UniProt Related Accession #
Q92673
GenBank Nucleotide #
NM_003105.5
NCBI Official Full Name
sortilin-related receptor preproprotein
NCBI Official Synonym Full Names
sortilin-related receptor, L(DLR class) A repeats containing
NCBI Official Symbol
SORL1
NCBI Official Synonym Symbols
LR11; LRP9; SORLA; gp250; SorLA-1; C11orf32
UniProt Protein Name
Sortilin-related receptor
UniProt Synonym Protein Names
Low-density lipoprotein receptor relative with 11 ligand-binding repeats; LDLR relative with 11 ligand-binding repeats; LR11; SorLA-1; Sorting protein-related receptor containing LDLR class A repeats; SorLA
Protein Family
Sortilin-related receptor
UniProt Gene Name
SORL1
UniProt Synonym Gene Names
C11orf32; LDLR relative with 11 ligand-binding repeats; LR11; SorLA
UniProt Entry Name
SORL_HUMAN
NCBI Protein Information
sortilin-related receptor; mosaic protein LR11; LDLR relative with 11 ligand-binding repeats; sortilin-related receptor, L(DLR class) A repeats-containing; sorting protein-related receptor containing LDLR class A repeats; low-density lipoprotein receptor relative with 11 ligand-binding repeats

NCBI Summary for SORL1

This gene encodes a mosaic protein that belongs to at least two families: the vacuolar protein sorting 10 (VPS10) domain-containing receptor family, and the low density lipoprotein receptor (LDLR) family. The encoded protein also contains fibronectin type III repeats and an epidermal growth factor repeat. The encoded protein is translated as a preproprotein and likely plays roles in endocytosis and sorting. There may be an association between expression of this locus and Alzheimer's Disease.[provided by RefSeq, Sep 2010]
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UniProt Comments for SORL1

SORL1: Likely to be a multifunctional endocytic receptor, that may be implicated in the uptake of lipoproteins and of proteases. Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. Binds the receptor- associated protein (RAP). Could play a role in cell-cell interaction. Belongs to the VPS10-related sortilin family. SORL1 subfamily.

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.2-q24.2

Cellular Component: Golgi apparatus; extracellular space; Golgi cisterna; integral to plasma membrane; endoplasmic reticulum; early endosome; trans-Golgi network; multivesicular body; recycling endosome; cell soma; membrane; nuclear envelope lumen; endosome

Molecular Function: ADP-ribosylation factor binding; protein binding; transmembrane receptor activity; beta-amyloid binding; low-density lipoprotein binding

Biological Process: cholesterol metabolic process; negative regulation of MAP kinase activity; receptor-mediated endocytosis; protein targeting to lysosome; cell migration; protein maturation; lipid transport; signal transduction; negative regulation of neurogenesis; negative regulation of protein oligomerization; cell proliferation; regulation of smooth muscle cell migration; positive regulation of protein catabolic process; post-Golgi vesicle-mediated transport; protein targeting to Golgi; negative regulation of protein binding; protein retention in Golgi; protein targeting

Disease: Alzheimer Disease
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Research Articles on SORL1

1. the SORL1 SNP rs985421 may alter the risk for sporadic Alzheimer disease and amnestic mild cognitive impairment in the Han Chinese population.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SORL1 antibody

 Reference Product  PubMed Publications
 BACE1 antibody  >9 publications with SORL1 and BACE1
 FURIN antibody  >4 publications with SORL1 and FURIN
 GGA1 antibody  >2 publications with SORL1 and GGA1
 OPCML antibody  >1 publications with SORL1 and OPCML
 GGA2 antibody  >1 publications with SORL1 and GGA2

Diseases associated with anti-SORL1 antibody

 Disease Name  Pubmed Publications
 Alzheimer Disease Antibodies  >104 publications with SORL1 and Alzheimer Disease
 Nervous System Diseases Antibodies  >103 publications with SORL1 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >15 publications with SORL1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >13 publications with SORL1 and Atherosclerosis
 Disease Models, Animal Antibodies  >10 publications with SORL1 and Disease Models, Animal
 Nerve Degeneration Antibodies  >5 publications with SORL1 and Nerve Degeneration
 Heart Diseases Antibodies  >4 publications with SORL1 and Heart Diseases
 Necrosis Antibodies  >4 publications with SORL1 and Necrosis
 Hyperplasia Antibodies  >3 publications with SORL1 and Hyperplasia
 Inflammation Antibodies  >2 publications with SORL1 and Inflammation

Organs/Tissues associated with anti-SORL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >81 publications with SORL1 and Brain
 Blood Antibodies  >27 publications with SORL1 and Blood
 Nerve Antibodies  >20 publications with SORL1 and Nerve
 Vascular Antibodies  >19 publications with SORL1 and Vascular
 Muscle Antibodies  >14 publications with SORL1 and Muscle
 Kidney Antibodies  >9 publications with SORL1 and Kidney
 Liver Antibodies  >7 publications with SORL1 and Liver
 Heart Antibodies  >6 publications with SORL1 and Heart
 Embryonic Tissue Antibodies  >4 publications with SORL1 and Embryonic Tissue
 Lung Antibodies  >3 publications with SORL1 and Lung
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