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CHGA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245453

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active… More details

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Product Overview

Product Name
CHGA / Chromogranin A, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 3A5] (IgG1,k) to Human CHGA / Chromogranin A
Product Gene Name
Product Synonym Names
Anti-CHGA / Chromogranin A Antibody (aa19-131, clone 3A5) IHC-plus; CHGA; Chromogranin A; Chromogranin-A; Pancreastatin; Pituitary secretory protein I; Vasostatin I; SP-I; Vasostatin II; Human CHGA; Chromogranin A
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3A5
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4, 0.1% sodium azide
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
50,688 Da
OMIM
118910
3D Structure
ModBase 3D Structure for P10645
Target Species
Human
Immunogen Description
Recombinant human Chromogranin A (19-131aa) purified from E Coli. NCBI Accession No.: NP_001266.
Immunogen Type
Recombinant protein
Immunogen
CHGA / Chromogranin A antibody was raised against recombinant human Chromogranin A (19-131aa) purified from E Coli. NCBI Accession No.: NP_001266.
Antigen Modification
aa19-131
Applications Tested/Suitable for anti-CHGA antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CHGA antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-CHGA antibody

Anti-CHGA / Chromogranin A antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-CHGA Monoclonal Antibody – Cat# MBS245453

Immunohistochemistry (IHC) of anti-CHGA antibody

Anti-CHGA / Chromogranin A antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-CHGA Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245453

Western Blot (WB) of anti-CHGA antibody

Cell lysates of A549, 293T and mouse kidney (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Chromogranin A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-CHGA Monoclonal Antibody, Western Blot (WB) – Cat# MBS245453

Other Notes

Small volumes of anti-CHGA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHGA purchase

MBS245453 is an antibody targeting CHGA. Also known as Mouse Monoclonal [clone 3A5] (IgG1,k) to Human CHGA / Chromogranin A. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CHGA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
chromogranin-A isoform 1 preproprotein
NCBI Official Synonym Full Names
chromogranin A (parathyroid secretory protein 1)
NCBI Official Symbol
CHGA
NCBI Official Synonym Symbols
CGA
UniProt Protein Name
Chromogranin-A
UniProt Synonym Protein Names
Pituitary secretory protein I; SP-I
Protein Family
UniProt Gene Name
CHGA
UniProt Synonym Gene Names
CgA; SP-I
UniProt Entry Name
CMGA_HUMAN
NCBI Protein Information
chromogranin-A; SP-I; catestatin; chromofungin; pituitary secretory protein I; parathyroid secretory protein 1; betagranin (N-terminal fragment of chromogranin A)

NCBI Summary for CHGA

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
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UniProt Comments for CHGA

CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells.

Protein type: Secreted; Vesicle; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: perinuclear region of cytoplasm; extracellular region; secretory granule

Biological Process: regulation of blood pressure
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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