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HSP90 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245471

MBS245471 is an antibody targeting HSP90. Also known as Mouse Monoclonal [clone 4F10] (IgG2b,k) to Human HSP90 / Heat Shock Protein 90. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
HSP90 / Heat Shock Protein 90, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 4F10] (IgG2b,k) to Human HSP90 / Heat Shock Protein 90
Product Gene Name
Product Synonym Names
Anti-HSP90 / Heat Shock Protein 90 Antibody (aa1-732, clone 4F10) IHC-plus; Heat Shock Protein 90; Human HSP90; Heat Shock Protein 90
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
4F10
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4, 0.1% sodium azide
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Target Species
Human
Immunogen Description
Recombinant human Hsp90 (1-732aa) purified from E Coli.
Immunogen Type
Recombinant protein
Immunogen
Recombinant human Hsp90 (1-732aa) purified from E Coli.
Antigen Modification
aa1-732
Applications Tested/Suitable for anti-HSP90 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-HSP90 antibody
ELISA, IHC-P (5 ug/ml), WB (1:1000 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-HSP90 antibody

Anti-HSP90 / Heat Shock Protein 90 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-HSP90 Monoclonal Antibody – Cat# MBS245471

Immunohistochemistry (IHC) of anti-HSP90 antibody

Anti-HSP90 / Heat Shock Protein 90 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-HSP90 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245471

Western Blot (WB) of anti-HSP90 antibody

Cell lysates of HeLa (each 20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp90(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.
Mouse Anti-HSP90 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245471

Other Notes

Small volumes of anti-HSP90 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSP90. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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