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CFL1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245491

MBS245491 is an antibody targeting CFL1. Also known as Mouse Monoclonal [clone AT1C1] (IgG1,k) to Human CFL1 / Cofilin. Reactive in Human. Host: Mouse. More details

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Product Overview

Product Name
CFL1 / Cofilin, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone AT1C1] (IgG1,k) to Human CFL1 / Cofilin
Product Gene Name
anti-CFL1 antibody
Product Synonym Names
Anti-CFL1 / Cofilin Antibody (aa1-166, clone AT1C1) IHC-plus; CFL1; 18 kDa phosphoprotein; Cofilin; CFL; Cofilin 1 (non-muscle); Cofilin; non-muscle isoform; p18; Human CFL1; Cofilin
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT1C1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4, 0.1% sodium azide
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
18,502 Da
OMIM
601442
3D Structure
ModBase 3D Structure for P23528
Target Species
Human
Immunogen Description
Recombinant human Cofilin 1 (1-166aa) purified from E Coli. NCBI Accession No.: NP_005498.
Immunogen Type
Recombinant protein
Immunogen
CFL1 / Cofilin antibody was raised against recombinant human Cofilin 1 (1-166aa) purified from E Coli. NCBI Accession No.: NP_005498.
Antigen Modification
aa1-166
Applications Tested/Suitable for anti-CFL1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CFL1 antibody
ELISA, IHC-P (10 ug/ml), WB (1:1000 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-CFL1 antibody

Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-CFL1 Monoclonal Antibody – Cat# MBS245491

Immunohistochemistry (IHC) of anti-CFL1 antibody

Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-CFL1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245491

Western Blot (WB) of anti-CFL1 antibody

The lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-CFL1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245491

Other Notes

Small volumes of anti-CFL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CFL1. It may not necessarily be applicable to this product.

NCBI GI #
5031635
NCBI GeneID
1072
NCBI Accession #
NP_005498.1
UniProt Primary Accession #
P23528
UniProt Secondary Accession #
Q53Y87; Q9UCA2; B3KUQ1
UniProt Related Accession #
P23528
GenBank Nucleotide #
NM_005507.2
NCBI Official Full Name
cofilin-1
NCBI Official Synonym Full Names
cofilin 1 (non-muscle)
NCBI Official Symbol
CFL1
NCBI Official Synonym Symbols
CFL; HEL-S-15
UniProt Protein Name
Cofilin-1
UniProt Synonym Protein Names
18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform
Protein Family
Cofilin
UniProt Gene Name
CFL1
UniProt Synonym Gene Names
CFL; p18
UniProt Entry Name
COF1_HUMAN
NCBI Protein Information
cofilin-1; p18; 18 kDa phosphoprotein; cofilin, non-muscle isoform; epididymis secretory protein Li 15

NCBI Summary for CFL1

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
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UniProt Comments for CFL1

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Cytoskeletal

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; nuclear matrix; membrane; cytoplasm; intercellular junction; nucleus; vesicle

Molecular Function: protein binding; actin binding

Biological Process: platelet activation; axon guidance; actin filament depolymerization; positive regulation of actin filament depolymerization; response to virus; negative regulation of cell size; cytoskeleton organization and biogenesis; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; cytokinesis after mitosis; establishment of cell polarity; protein import into nucleus; ephrin receptor signaling pathway; innate immune response; regulation of cell morphogenesis; blood coagulation; actin cytoskeleton organization and biogenesis; neural crest cell migration; negative regulation of apoptosis
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Research Articles on CFL1

1. It provides a brief overview of the current knowledge about the central role of cofilin/actin rods formation, where its dysregulation and malfunction might be the trigger to neurodegeneration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CFL1 antibody

 Reference Product  PubMed Publications
 LIMK1 antibody  >16 publications with CFL1 and LIMK1
 SSH1 antibody  >6 publications with CFL1 and SSH1
 PFN1 antibody  >4 publications with CFL1 and PFN1
 ACTG1 antibody  >2 publications with CFL1 and ACTG1
 ACTB antibody  >2 publications with CFL1 and ACTB

Pathways associated with anti-CFL1 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHB-mediated Forward Signaling Pathway antibodies  EPHB-mediated Forward Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram

Diseases associated with anti-CFL1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >9 publications with CFL1 and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >9 publications with CFL1 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >7 publications with CFL1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >7 publications with CFL1 and Breast Neoplasms
 Inflammation Antibodies  >5 publications with CFL1 and Inflammation
 Neoplasms, Experimental Antibodies  >3 publications with CFL1 and Neoplasms, Experimental
 Neoplasm Invasiveness Antibodies  >3 publications with CFL1 and Neoplasm Invasiveness
 Cardiovascular Diseases Antibodies  >2 publications with CFL1 and Cardiovascular Diseases
 Memory Disorders Antibodies  >2 publications with CFL1 and Memory Disorders
 Kidney Diseases Antibodies  >1 publications with CFL1 and Kidney Diseases

Organs/Tissues associated with anti-CFL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >18 publications with CFL1 and Brain
 Muscle Antibodies  >14 publications with CFL1 and Muscle
 Blood Antibodies  >12 publications with CFL1 and Blood
 Nerve Antibodies  >11 publications with CFL1 and Nerve
 Lung Antibodies  >8 publications with CFL1 and Lung
 Kidney Antibodies  >6 publications with CFL1 and Kidney
 Uterus Antibodies  >5 publications with CFL1 and Uterus
 Vascular Antibodies  >4 publications with CFL1 and Vascular
 Eye Antibodies  >3 publications with CFL1 and Eye
 Embryonic Tissue Antibodies  >3 publications with CFL1 and Embryonic Tissue
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