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OTUD7B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245517

MBS245517 is an antibody targeting OTUD7B. Also known as Mouse Monoclonal [clone 2B4] (IgG2b,k) to Human OTUD7B / Cezanne. Reactive in Human. Host: Mouse. More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
OTUD7B / Cezanne, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2B4] (IgG2b,k) to Human OTUD7B / Cezanne
Product Gene Name
Product Synonym Names
Anti-OTUD7B / Cezanne Antibody (clone 2B4) IHC-plus; OTUD7B; CEZANNE; OTU domain containing 7B; ZA20D1; Zinc finger protein Cezanne; Human OTUD7B; Cezanne
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2B4
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.42 mg/ml (lot specific)
Specificity
Human OTUD7B
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
88,947 Da
OMIM
611748
3D Structure
ModBase 3D Structure for Q6GQQ9
Target Species
Human
Immunogen Description
Amino acids 744 to 843 of NP_064590, with a GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
OTUD7B / Cezanne antibody was raised against amino acids 744 to 843 of NP_064590, with a GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-OTUD7B antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-OTUD7B antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot with the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-OTUD7B antibody

Anti-OTUD7B antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-OTUD7B Monoclonal Antibody – Cat# MBS245517

ELISA (EIA) of anti-OTUD7B antibody

Detection limit for recombinant GST tagged OTUD7B is 0.3 ng/ml as a capture antibody.
Mouse Anti-OTUD7B Monoclonal Antibody, ELISA (EIA) – Cat# MBS245517

Other Notes

Small volumes of anti-OTUD7B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OTUD7B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
OTU domain-containing protein 7B
NCBI Official Synonym Full Names
OTU deubiquitinase 7B
NCBI Official Symbol
OTUD7B
NCBI Official Synonym Symbols
ZA20D1; CEZANNE
UniProt Protein Name
OTU domain-containing protein 7B
UniProt Synonym Protein Names
Cellular zinc finger anti-NF-kappa-B protein; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne
UniProt Gene Name
OTUD7B
UniProt Synonym Gene Names
ZA20D1
UniProt Entry Name
OTU7B_HUMAN
NCBI Protein Information
OTU domain-containing protein 7B; OTU domain containing 7B; zinc finger protein Cezanne; zinc finger, A20 domain containing 1; cellular zinc finger anti-NF-kappaB Cezanne; zinc finger A20 domain-containing protein 1; cellular zinc finger anti-NF-kappa-B protein

UniProt Comments for OTUD7B

Cezanne: cellular zinc finger anti-NF-kappaB, a negative regulator of NF-kappaB. A novel deubiquitinating enzyme that belongs to the ovarian tumor protein (OTU) superfamily.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: mucosal immune response; negative regulation of interleukin-8 production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of I-kappaB kinase/NF-kappaB cascade; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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