• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PRKRA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245583

MBS245583 is an antibody targeting PRKRA. Also known as Mouse Monoclonal [clone 1B9-1A7] (IgG1,k) to Human PRKRA / PACT. Reactive in Human. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
PRKRA / PACT, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1B9-1A7] (IgG1,k) to Human PRKRA / PACT
Product Gene Name
anti-PRKRA antibody
Product Synonym Gene Name
PACT
Product Synonym Names
Anti-PRKRA / PACT Antibody (clone 1B9-1A7) IHC-plus; PRKRA; DYT16; HSD14; PKR-associating protein X; PACT; PKR-associated protein X; Human PRKRA; PACT
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1B9-1A7
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human PACT
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
31,635 Da
OMIM
gene 612067
3D Structure
ModBase 3D Structure for O75569
Target Species
Human
Immunogen Description
PRKRA (AAH09470, 1 a.a. ~ 314 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
PRKRA / PACT antibody was raised against pRKRA (AAH09470, 1 a.a. ~ 314 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-PRKRA antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
Application Notes for anti-PRKRA antibody
ELISA, IHC-P (2.5 ug/ml), IP, WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate, Cell lysate, Transfected lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-PRKRA antibody

Anti-PRKRA / PACT antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Mouse Anti-PRKRA Monoclonal Antibody – Cat# MBS245583

Western Blot (WB) of anti-PRKRA antibody

Western blot of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody, clone 1B9-1A7.
Mouse Anti-PRKRA Monoclonal Antibody, Western Blot (WB) – Cat# MBS245583

Western Blot (WB) of anti-PRKRA antibody

PRKRA monoclonal antibody, clone 1B9-1A7. Western blot of PRKRA expression in human liver.
Mouse Anti-PRKRA Monoclonal Antibody, Western Blot (WB) – Cat# MBS245583

Immunoprecipitation (IP) of anti-PRKRA antibody

Immunoprecipitation of PRKRA transfected lysate using anti-PRKRA monoclonal antibody and Protein A Magnetic Bead.
Mouse Anti-PRKRA Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS245583

ELISA (EIA) of anti-PRKRA antibody

Detection limit for recombinant GST tagged PRKRA is approximately 0.03 ng/ml as a capture antibody.
Mouse Anti-PRKRA Monoclonal Antibody, ELISA (EIA) – Cat# MBS245583

Other Notes

Small volumes of anti-PRKRA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKRA. It may not necessarily be applicable to this product.

NCBI GI #
4505581
NCBI GeneID
8575
NCBI Accession #
NP_003681.1
UniProt Primary Accession #
O75569
UniProt Secondary Accession #
Q53G24; Q6X7T5; Q8NDK4; A8K3I6
UniProt Related Accession #
O75569
GenBank Nucleotide #
NM_003690.4
NCBI Official Full Name
interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 1
NCBI Official Synonym Full Names
protein kinase, interferon-inducible double stranded RNA dependent activator
NCBI Official Symbol
PRKRA
NCBI Official Synonym Symbols
RAX; PACT; DYT16; HSD14
UniProt Protein Name
Interferon-inducible double-stranded RNA-dependent protein kinase activator A
UniProt Synonym Protein Names
PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; Protein kinase, interferon-inducible double-stranded RNA-dependent activator
Protein Family
Interferon-inducible double-stranded RNA-dependent protein kinase activator
UniProt Gene Name
PRKRA
UniProt Synonym Gene Names
PACT; RAX
UniProt Entry Name
PRKRA_HUMAN
NCBI Protein Information
interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA-dependent activator; protein kinase, interferon-inducible double-stranded RNA-dependent activator; interferon-inducible double stranded RNA-dependent protein kinase activator A

NCBI Summary for PRKRA

This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

UniProt Comments for PRKRA

PACT: a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported.

Protein type: Activator; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: nucleoplasm; membrane; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding; protein homodimerization activity; enzyme activator activity

Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; skeletal morphogenesis; response to virus; outer ear morphogenesis; gene expression; immune response; middle ear morphogenesis; RNA interference, production of siRNA; protein amino acid phosphorylation

Disease: Dystonia 16
Show more

Research Articles on PRKRA

1. NS1 prevents PACT from interacting with an essential component of the virus.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PRKRA antibody

 Reference Product  PubMed Publications
 DICER1 antibody  >8 publications with PRKRA and DICER1
 EIF2C2 antibody  >6 publications with PRKRA and EIF2C2
 TARBP2 antibody  >4 publications with PRKRA and TARBP2

Pathways associated with anti-PRKRA antibody

 Products by Pathway  Pathway Diagram
 Ceramide Signaling Pathway antibodies  Ceramide Signaling Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 MicroRNA (miRNA) Biogenesis Pathway antibodies  MicroRNA (miRNA) Biogenesis Pathway Diagram
 Regulatory RNA Pathways antibodies  Regulatory RNA Pathways Diagram
 Small Interfering RNA (siRNA) Biogenesis Pathway antibodies  Small Interfering RNA (siRNA) Biogenesis Pathway Diagram

Diseases associated with anti-PRKRA antibody

 Disease Name  Pubmed Publications
 Dystonia Antibodies  >16 publications with PRKRA and Dystonia
 Dystonic Disorders Antibodies  >10 publications with PRKRA and Dystonic Disorders
 Parkinsonian Disorders Antibodies  >5 publications with PRKRA and Parkinsonian Disorders
 Craniofacial Abnormalities Antibodies  >3 publications with PRKRA and Craniofacial Abnormalities
 Disease Models, Animal Antibodies  >2 publications with PRKRA and Disease Models, Animal
 Necrosis Antibodies  >1 publications with PRKRA and Necrosis
 Kidney Diseases Antibodies  >1 publications with PRKRA and Kidney Diseases
 Infertility, Male Antibodies  >1 publications with PRKRA and Infertility, Male
 Hyperplasia Antibodies  >1 publications with PRKRA and Hyperplasia
 Inflammation Antibodies  >1 publications with PRKRA and Inflammation

Organs/Tissues associated with anti-PRKRA antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with PRKRA and Brain
 Ear Antibodies  >6 publications with PRKRA and Ear
 Blood Antibodies  >5 publications with PRKRA and Blood
 Liver Antibodies  >5 publications with PRKRA and Liver
 Muscle Antibodies  >4 publications with PRKRA and Muscle
 Ganglia Antibodies  >3 publications with PRKRA and Ganglia
 Bone Antibodies  >2 publications with PRKRA and Bone
 Eye Antibodies  >2 publications with PRKRA and Eye
 Testis Antibodies  >2 publications with PRKRA and Testis
 Kidney Antibodies  >1 publications with PRKRA and Kidney
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000