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PTPRN Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245594

PTPRN / IA-2 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP… More details

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Product Overview

Product Name
PTPRN / IA-2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 8E3] (IgG2a,k) to Human PTPRN / IA-2
Product Gene Name
anti-PTPRN antibody
Product Synonym Gene Name
IA-2
Product Synonym Names
PTPRN; ICA512; IA2; ICA 512; Islet antigen 2; Islet cell antigen 2; Islet cell autoantigen 3; IA-2; Ia-2 antigen; ICA3; PTP-NP-2; PTP IA-2; R-PTP-N; IA-2/PTP; Islet cell antigen 512; Human PTPRN; IA-2
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
8E3
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4
Purity/Purification
Purified from ascites by Protein A
Concentration
0.44 mg/ml (lot specific)
Specificity
Human PTPRN
Preparation and Storage
Store at 4°C for ahort term storage. For long term storage, aliquot and store at -20°C, avoid repeated freeze/thaw cycles.
OMIM
601773
3D Structure
ModBase 3D Structure for Q16849
Target Species
Human
Immunogen
Raised against recombinant protein encoding aa 205-311 of NP_002837.
Immunogen Species
Antibody was raised against Human.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-PTPRN antibody

Family: Protein Phosphatase; Subfamily: R8
Applications Tested/Suitable for anti-PTPRN antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PTPRN antibody
ELISA, IHC-P (5 ug/ml), WB
Usage: Western Blot: Transfected lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-PTPRN antibody

Anti-PTPRN antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-PTPRN Monoclonal Antibody – Cat# MBS245594

Western Blot (WB) of anti-PTPRN antibody

Western blot of PTPRN expression in transfected 293T cell line by PTPRN monoclonal antibody, clone 8E3.
Mouse Anti-PTPRN Monoclonal Antibody, Western Blot (WB) – Cat# MBS245594

Other Notes

Small volumes of anti-PTPRN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTPRN purchase

MBS245594 is an antibody targeting PTPRN. Also known as Mouse Monoclonal [clone 8E3] (IgG2a,k) to Human PTPRN / IA-2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PTPRN. It may not necessarily be applicable to this product.

NCBI GI #
4506321
NCBI GeneID
5798
NCBI Accession #
NP_002837.1
UniProt Primary Accession #
Q16849
UniProt Secondary Accession #
Q08319; Q53QD6; Q6NSL1; B4DK12; F5GZS3
UniProt Related Accession #
Q16849
GenBank Nucleotide #
NM_002846.3
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase-like N isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, N
NCBI Official Symbol
PTPRN
NCBI Official Synonym Symbols
IA2; IA-2; ICA512; R-PTP-N; IA-2/PTP
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase-like N
UniProt Synonym Protein Names
Islet cell antigen 512; ICA 512; Islet cell autoantigen 3; PTP IA-2
Protein Family
Receptor-type tyrosine-protein phosphatase
UniProt Gene Name
PTPRN
UniProt Synonym Gene Names
ICA3; ICA512; R-PTP-N; ICA 512
UniProt Entry Name
PTPRN_HUMAN
NCBI Protein Information
receptor-type tyrosine-protein phosphatase-like N; ICA 512; PTP IA-2; islet cell antigen 2; islet cell antigen 512; islet cell autoantigen 3; protein tyrosine phosphatase-like N

NCBI Summary for PTPRN

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single catalytic domain, and thus represents a receptor-type PTP. This PTP was found to be an autoantigen that is reactive with insulin-dependent diabetes mellitus (IDDM) patient sera, and thus may be a potential target of autoimmunity in diabetes mellitus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]
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UniProt Comments for PTPRN

PTPRN: Implicated in neuroendocrine secretory processes. May be involved in processes specific for neurosecretory granules, such as their biogenesis, trafficking or regulated exocytosis or may have a general role in neuroendocrine functions. Seems to lack intrinsic enzyme activity. May play a role in the regulation of secretory granules via its interaction with SNTB2. Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor protein phosphatase, tyrosine; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q35-q36.1

Cellular Component: integral to membrane

Molecular Function: GTPase binding; protein binding; spectrin binding; protein tyrosine phosphatase activity

Biological Process: response to reactive oxygen species; response to cAMP; insulin secretion; response to estrogen stimulus; cytokine and chemokine mediated signaling pathway; response to glucose stimulus; protein amino acid dephosphorylation; response to insulin stimulus
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Research Articles on PTPRN

1. Humoral responses to islet antigen-2 and zinc transporter 8 are attenuated in patients carrying HLA-A*24 alleles at the onset of type 1 diabetes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PTPRN antibody

 Reference Product  PubMed Publications
 PTPRT antibody  >23 publications with PTPRN and PTPRT
 INS antibody  >8 publications with PTPRN and INS
 MADD antibody  >1 publications with PTPRN and MADD
 PTPRS antibody  >1 publications with PTPRN and PTPRS
 GAD2 antibody  >1 publications with PTPRN and GAD2

Pathways associated with anti-PTPRN antibody

 Products by Pathway  Pathway Diagram
 Type I Diabetes Mellitus Pathway antibodies  Type I Diabetes Mellitus Pathway Diagram
 Type I Diabetes Mellitus Pathway antibodies  Type I Diabetes Mellitus Pathway Diagram

Diseases associated with anti-PTPRN antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >196 publications with PTPRN and Immune System Diseases
 Nervous System Diseases Antibodies  >10 publications with PTPRN and Nervous System Diseases
 Disease Models, Animal Antibodies  >7 publications with PTPRN and Disease Models, Animal
 Brain Diseases Antibodies  >4 publications with PTPRN and Brain Diseases
 Kidney Diseases Antibodies  >3 publications with PTPRN and Kidney Diseases
 Weight Loss Antibodies  >2 publications with PTPRN and Weight Loss
 Inflammation Antibodies  >2 publications with PTPRN and Inflammation
 Liver Diseases Antibodies  >2 publications with PTPRN and Liver Diseases
 Congenital Abnormalities Antibodies  >1 publications with PTPRN and Congenital Abnormalities
 Edema Antibodies  >1 publications with PTPRN and Edema

Organs/Tissues associated with anti-PTPRN antibody

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >131 publications with PTPRN and Pancreas
 Brain Antibodies  >29 publications with PTPRN and Brain
 Nerve Antibodies  >6 publications with PTPRN and Nerve
 Lung Antibodies  >6 publications with PTPRN and Lung
 Kidney Antibodies  >4 publications with PTPRN and Kidney
 Pituitary Gland Antibodies  >4 publications with PTPRN and Pituitary Gland
 Eye Antibodies  >4 publications with PTPRN and Eye
 Muscle Antibodies  >3 publications with PTPRN and Muscle
 Embryonic Tissue Antibodies  >2 publications with PTPRN and Embryonic Tissue
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