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RRM2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245718

Ribonucleotide reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. It is composed of 2 non-identical subunits, proteins M1 and M2. Synthesis of M2 is regulated in a cell-cycle dependent fashion. More details

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Product Overview

Product Name
RRM2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1E1] (IgG1,k) to Human RRM2
Product Gene Name
anti-RRM2 antibody
Product Synonym Names
Anti-RRM2 Antibody (clone 1E1) IHC-plus; RRM2; Ribonucleotide reductase M2; RR2; RR2M; Human RRM2
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1E1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.43 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
51,093 Da
OMIM
180390
3D Structure
ModBase 3D Structure for P31350
Target Species
Human
Immunogen Description
RRM2 (NP_001025, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
RRM2 antibody was raised against rRM2 (NP_001025, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-RRM2 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RRM2 antibody
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control HepG2. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Immunohistochemistry (IHC) of anti-RRM2 antibody

Anti-RRM2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-RRM2 Monoclonal Antibody – Cat# MBS245718

Immunohistochemistry (IHC) of anti-RRM2 antibody

Anti-RRM2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-RRM2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245718

Immunofluorescence (IF) of anti-RRM2 antibody

Immunofluorescence of monoclonal antibody to RRM2 on HeLa cell (antibody concentration 10 ug/ml).
Mouse Anti-RRM2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS245718

Western Blot (WB) of anti-RRM2 antibody

RRM2 monoclonal antibody clone 1E1. Western blot of RRM2 expression in HepG2.
Mouse Anti-RRM2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245718

ELISA (EIA) of anti-RRM2 antibody

Detection limit for recombinant GST tagged RRM2 is approximately 0.03 ng/ml as a capture antibody.
Mouse Anti-RRM2 Monoclonal Antibody, ELISA (EIA) – Cat# MBS245718

Other Notes

Small volumes of anti-RRM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RRM2 purchase

MBS245718 is an antibody targeting RRM2. Also known as Mouse Monoclonal [clone 1E1] (IgG1,k) to Human RRM2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RRM2. It may not necessarily be applicable to this product.

NCBI GI #
4557845
NCBI GeneID
6241
NCBI Accession #
NP_001025.1
UniProt Primary Accession #
P31350
UniProt Secondary Accession #
Q5WRU7; B2R9B5; J3KP43
UniProt Related Accession #
P31350
GenBank Nucleotide #
NM_001034.3
NCBI Official Full Name
ribonucleoside-diphosphate reductase subunit M2 isoform 2
NCBI Official Synonym Full Names
ribonucleotide reductase M2
NCBI Official Symbol
RRM2
NCBI Official Synonym Symbols
R2; RR2; RR2M
UniProt Protein Name
Ribonucleoside-diphosphate reductase subunit M2
UniProt Synonym Protein Names
Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit
Protein Family
Ribonucleoside-diphosphate reductase
UniProt Gene Name
RRM2
UniProt Synonym Gene Names
RR2
UniProt Entry Name
RIR2_HUMAN
NCBI Protein Information
ribonucleoside-diphosphate reductase subunit M2; ribonucleotide reductase small chain; ribonucleotide reductase small subunit; ribonucleotide reductase M2 polypeptide

NCBI Summary for RRM2

This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009]
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UniProt Comments for RRM2

RRM2: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. Belongs to the ribonucleoside diphosphate reductase small chain family.

Protein type: Nucleotide Metabolism - pyrimidine; Oxidoreductase; Nucleotide Metabolism - purine; EC 1.17.4.1; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 2p25-p24

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; metal ion binding; ribonucleoside-diphosphate reductase activity

Biological Process: G1/S-specific transcription in mitotic cell cycle; deoxyribonucleoside diphosphate metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; deoxyribonucleotide biosynthetic process; protein heterotetramerization; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle
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Research Articles on RRM2

1. these findings suggest that RRM2 plays a crucial role in gastric tumorigenesis and may serve as a potential prognostic marker and therapeutic target in gastric cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RRM2 antibody

 Reference Product  PubMed Publications
 RRM1 antibody  >117 publications with RRM2 and RRM1
 E2F1 antibody  >4 publications with RRM2 and E2F1
 CDK1 antibody  >3 publications with RRM2 and CDK1
 CCNA2 antibody  >2 publications with RRM2 and CCNA2
 PCNA antibody  >2 publications with RRM2 and PCNA
 TYMS antibody  >2 publications with RRM2 and TYMS
 CDC6 antibody  >1 publications with RRM2 and CDC6

Pathways associated with anti-RRM2 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 E2F Mediated Regulation Of DNA Replication Pathway antibodies  E2F Mediated Regulation Of DNA Replication Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 Fluoropyrimidine Activity Pathway antibodies  Fluoropyrimidine Activity Pathway Diagram
 G1/S Transition Pathway antibodies  G1/S Transition Pathway Diagram
 G1/S-Specific Transcription Pathway antibodies  G1/S-Specific Transcription Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with anti-RRM2 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >32 publications with RRM2 and Adenocarcinoma
 Breast Neoplasms Antibodies  >15 publications with RRM2 and Breast Neoplasms
 Lung Neoplasms Antibodies  >15 publications with RRM2 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >13 publications with RRM2 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >10 publications with RRM2 and Nervous System Diseases
 Colorectal Neoplasms Antibodies  >9 publications with RRM2 and Colorectal Neoplasms
 Liver Neoplasms Antibodies  >5 publications with RRM2 and Liver Neoplasms
 Brain Diseases Antibodies  >5 publications with RRM2 and Brain Diseases
 Ovarian Neoplasms Antibodies  >5 publications with RRM2 and Ovarian Neoplasms
 Disease Models, Animal Antibodies  >5 publications with RRM2 and Disease Models, Animal

Organs/Tissues associated with anti-RRM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >20 publications with RRM2 and Lung
 Blood Antibodies  >13 publications with RRM2 and Blood
 Brain Antibodies  >10 publications with RRM2 and Brain
 Liver Antibodies  >8 publications with RRM2 and Liver
 Bone Antibodies  >7 publications with RRM2 and Bone
 Lymph Node Antibodies  >6 publications with RRM2 and Lymph Node
 Prostate Antibodies  >6 publications with RRM2 and Prostate
 Lymph Antibodies  >6 publications with RRM2 and Lymph
 Muscle Antibodies  >5 publications with RRM2 and Muscle
 Nerve Antibodies  >5 publications with RRM2 and Nerve
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