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HSET Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245726

MBS245726 is an antibody targeting HSET. Also known as Mouse Monoclonal [clone 2B9] (IgG2a,k) to Human HSET / KIFC1. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
HSET / KIFC1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2B9] (IgG2a,k) to Human HSET / KIFC1
Product Gene Name
Product Synonym Gene Name
KIFC1
Product Synonym Names
Anti-HSET / KIFC1 Antibody (clone 2B9) IHC-plus; KIFC1; HSET; KNSL2; Kinesin-like 2; Kinesin-related protein; Kinesin family member C1; Kinesin-like protein KIFC1; Kinesin-like protein 2; Kinesin-related protein HSET; Human HSET; KIFC1
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2B9
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human KIFC1
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
73,748 Da
OMIM
603763
3D Structure
ModBase 3D Structure for Q9BW19
Target Species
Human
Immunogen Description
KIFC1 (AAH00712, 53 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
HSET / KIFC1 antibody was raised against kIFC1 (AAH00712, 53 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-HSET antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-HSET antibody
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot with the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-HSET antibody

Anti-KIFC1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-HSET Monoclonal Antibody – Cat# MBS245726

Immunofluorescence (IF) of anti-HSET antibody

Immunofluorescence of monoclonal antibody to KIFC1 on HeLa cells.
Mouse Anti-HSET Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS245726

ELISA (EIA) of anti-HSET antibody

Detection limit for recombinant GST tagged KIFC1 is approximately 1 ng/ml as a capture antibody.
Mouse Anti-HSET Monoclonal Antibody, ELISA (EIA) – Cat# MBS245726

Other Notes

Small volumes of anti-HSET antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSET. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
kinesin-like protein KIFC1
NCBI Official Synonym Full Names
kinesin family member C1
NCBI Official Symbol
KIFC1
NCBI Official Synonym Symbols
HSET; KNSL2
UniProt Protein Name
Kinesin-like protein KIFC1
UniProt Synonym Protein Names
Kinesin-like protein 2; Kinesin-related protein HSET
UniProt Gene Name
KIFC1
UniProt Synonym Gene Names
HSET; KNSL2
UniProt Entry Name
KIFC1_HUMAN
NCBI Protein Information
kinesin-like protein KIFC1; kinesin-like 2; kinesin-like protein 2; kinesin-related protein HSET

UniProt Comments for HSET

KIFC1: Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Belongs to the kinesin-like protein family. NCD subfamily. Binds NUBP1 and NUBP2.

Protein type: Motility/polarity/chemotaxis; Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: microtubule; kinesin complex; membrane; early endosome; microtubule organizing center; spindle; nucleus

Molecular Function: minus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: mitotic sister chromatid segregation; cell division; metabolic process; spindle assembly; blood coagulation; mitotic metaphase plate congression; microtubule-based movement
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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