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RNF144B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245745

MBS245745 is an antibody targeting RNF144B. Also known as Mouse Monoclonal [clone 4F1] (IgG2a,k) to Human RNF144B. Reactive in Human. Host: Mouse. More details

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Product Overview

Product Name
RNF144B, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 4F1] (IgG2a,k) to Human RNF144B
Product Gene Name
anti-RNF144B antibody
Product Synonym Names
Anti-RNF144B Antibody (clone 4F1) IHC-plus; RNF144B; BA528A10.3; IBRDC2; p53RFP; IBR domain containing 2; PIR2; Ring finger protein 144B; Human RNF144B
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4F1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
23,856 Da
3D Structure
ModBase 3D Structure for Q7Z419
Target Species
Human
Immunogen Description
IBRDC2 (NP_877434, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
RNF144B antibody was raised against iBRDC2 (NP_877434, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-RNF144B antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RNF144B antibody
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Western Blot (Cell lysate) - positive control IMR-32. Immunofluorescence (10 ug/ml) - positive control HeLa cells.

Immunohistochemistry (IHC) of anti-RNF144B antibody

Anti-RNF144B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-RNF144B Monoclonal Antibody – Cat# MBS245745

Immunohistochemistry (IHC) of anti-RNF144B antibody

Anti-RNF144B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-RNF144B Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245745

Immunohistochemistry (IHC) of anti-RNF144B antibody

Anti-RNF144B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-RNF144B Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245745

Immunofluorescence (IF) of anti-RNF144B antibody

Immunofluorescence of monoclonal antibody to IBRDC2 on HeLa cell (antibody concentration 10 ug/ml).
Mouse Anti-RNF144B Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS245745

Western Blot (WB) of anti-RNF144B antibody

RNF144B monoclonal antibody, clone 4F1 Western blot of IBRDC2 expression in IMR-32.
Mouse Anti-RNF144B Monoclonal Antibody, Western Blot (WB) – Cat# MBS245745

Other Notes

Small volumes of anti-RNF144B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RNF144B. It may not necessarily be applicable to this product.

NCBI GI #
50284696
NCBI GeneID
255488
NCBI Accession #
NP_877434.2
UniProt Primary Accession #
Q7Z419
UniProt Secondary Accession #
Q5TB85; Q6P4Q0; Q8N3R7; Q9BX38; B3KUA8; B4DZI2
UniProt Related Accession #
Q7Z419
GenBank Nucleotide #
NM_182757.3
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF144B
NCBI Official Synonym Full Names
ring finger protein 144B
NCBI Official Symbol
RNF144B
NCBI Official Synonym Symbols
PIR2; IBRDC2; p53RFP; bA528A10.3
UniProt Protein Name
E3 ubiquitin-protein ligase RNF144B
UniProt Synonym Protein Names
IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
RNF144B
UniProt Synonym Gene Names
IBRDC2; P53RFP
UniProt Entry Name
R144B_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase RNF144B; IBR domain containing 2; IBR domain-containing protein 2; p53-inducible RING finger protein

UniProt Comments for RNF144B

RNF144B: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se. Belongs to the RBR family. RNF144 subfamily.

Protein type: Ligase; Membrane protein, integral; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; mitochondrial membrane; integral to membrane; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: apoptosis; protein ubiquitination during ubiquitin-dependent protein catabolic process
Show more

Research Articles on RNF144B

1. findings substantiate PIR2/Rnf144b as a potentially critical component of epithelial homeostasis, acting downstream of DeltaNp63alpha to regulate cellular levels of p21(WAF1/CIP1) and DeltaNp63alpha

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RNF144B antibody

 Reference Product  PubMed Publications
 CDKN1A antibody  >2 publications with RNF144B and CDKN1A
 BAX antibody  >1 publications with RNF144B and BAX
 UBE2L3 antibody  >1 publications with RNF144B and UBE2L3
 UBE2L6 antibody  >1 publications with RNF144B and UBE2L6

Pathways associated with anti-RNF144B antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Gastric Cancer Network 2 Pathway antibodies  Gastric Cancer Network 2 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-RNF144B antibody

 Disease Name  Pubmed Publications
 Pain Antibodies  >1 publications with RNF144B and Pain

Organs/Tissues associated with anti-RNF144B antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with RNF144B and Lung
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