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APOO Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245767

MBS245767 is an antibody targeting APOO. Also known as Mouse Monoclonal [clone 2F1] (IgG1) to Human APOO / Apolipoprotein O. Reactive in Human, Mouse. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
APOO / Apolipoprotein O, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2F1] (IgG1) to Human APOO / Apolipoprotein O
Product Gene Name
Product Synonym Names
Anti-APOO / Apolipoprotein O Antibody (clone 2F1) IHC-plus; APOO; Apolipoprotein O; Brain my025; FAM121B; MYO25; My025; Protein FAM121B; Human APOO; Apolipoprotein O
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2F1
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Ascites fluid, 0.03% sodium azide. Sourced in Ascites.
Purity/Purification
Ascites
Specificity
Human APOO
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
Molecular Weight
20,199 Da
OMIM
300753
3D Structure
ModBase 3D Structure for Q9BUR5
Target Species
Human
Immunogen Description
Purified recombinant fragment of ApoO expressed in E Coli.
Immunogen
APOO / Apolipoprotein O antibody was raised against purified recombinant fragment of ApoO expressed in E Coli.
Applications Tested/Suitable for anti-APOO antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-APOO antibody
ELISA (1:10000), IHC-P (1:200), WB (1:500 - 1:2000)

Immunohistochemistry (IHC) of anti-APOO antibody

Anti-Apolipoprotein O antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Mouse Anti-APOO Monoclonal Antibody – Cat# MBS245767

Western Blot (WB) of anti-APOO antibody

Western blot of anti-ApoO monoclonal antibody against HepG2(1) and 3T3L1(2) cell lysate.
Mouse Anti-APOO Monoclonal Antibody, Western Blot (WB) – Cat# MBS245767

Other Notes

Small volumes of anti-APOO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for APOO. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
apolipoprotein O
NCBI Official Synonym Full Names
apolipoprotein O
NCBI Official Symbol
APOO
NCBI Official Synonym Symbols
My025; FAM121B
UniProt Protein Name
Apolipoprotein O
UniProt Synonym Protein Names
Protein FAM121B
Protein Family
UniProt Gene Name
APOO
UniProt Synonym Gene Names
FAM121B
UniProt Entry Name
APOO_HUMAN
NCBI Protein Information
apolipoprotein O; brain my025; family with sequence similarity 121B

NCBI Summary for APOO

This gene is a member of the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation. This protein may be involved in preventing lipid accumulation in the myocardium in obese and diabetic patients. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 4, 5, 12 and 16.[provided by RefSeq, Sep 2009]
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UniProt Comments for APOO

APOO: Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation. Belongs to the apolipoprotein O family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: extracellular space; mitochondrion; integral to membrane

Biological Process: lipid transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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