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OCRL Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245771

MBS245771 is an antibody targeting OCRL. Also known as Mouse Monoclonal [clone 4A6] (IgG2a,k) to Human OCRL. Reactive in Human. Host: Mouse. More details

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Product Overview

Product Name
OCRL, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 4A6] (IgG2a,k) to Human OCRL
Product Gene Name
anti-OCRL antibody
Product Synonym Names
Anti-OCRL Antibody (clone 4A6) IHC-plus; OCRL; NPHL2; OCRL-1; LOCR; OCRL1; Human OCRL
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4A6
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human OCRL
Preparation and Storage
Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.
Molecular Weight
103,227 Da
OMIM
phenotype 309000
3D Structure
ModBase 3D Structure for Q01968
Target Species
Human
Immunogen Description
OCRL (NP_000267, 146 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
OCRL antibody was raised against oCRL (NP_000267, 146 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Product Categories/Family for anti-OCRL antibody

Family: Inositol phosphatase
Applications Tested/Suitable for anti-OCRL antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-OCRL antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-OCRL antibody

Anti-OCRL antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-OCRL Monoclonal Antibody – Cat# MBS245771

Western Blot (WB) of anti-OCRL antibody

Western blot of OCRL expression in transfected 293T cell line by OCRL monoclonal antibody.
Mouse Anti-OCRL Monoclonal Antibody, Western Blot (WB) – Cat# MBS245771

Western Blot (WB) of anti-OCRL antibody

OCRL monoclonal antibody, Western blot of OCRL expression in HeLa NE.
Mouse Anti-OCRL Monoclonal Antibody, Western Blot (WB) – Cat# MBS245771

ELISA (EIA) of anti-OCRL antibody

Detection limit for recombinant GST tagged OCRL is approximately 0.1 ng/ml as a capture antibody.
Mouse Anti-OCRL Monoclonal Antibody, ELISA (EIA) – Cat# MBS245771

Other Notes

Small volumes of anti-OCRL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OCRL. It may not necessarily be applicable to this product.

NCBI GI #
13325072
NCBI GeneID
4952
NCBI Accession #
NP_000267.2
UniProt Primary Accession #
Q01968
UniProt Secondary Accession #
O60800; Q15684; Q15774; Q4VY09; Q4VY10; Q5JQF1; Q5JQF2; Q9UJG5; A6NKI1; A8KAP2; B7ZLX2
UniProt Related Accession #
Q01968
GenBank Nucleotide #
NM_000276.3
NCBI Official Full Name
inositol polyphosphate 5-phosphatase OCRL-1 isoform a
NCBI Official Synonym Full Names
oculocerebrorenal syndrome of Lowe
NCBI Official Symbol
OCRL
NCBI Official Synonym Symbols
LOCR; NPHL2; OCRL1; INPP5F; OCRL-1
UniProt Protein Name
Inositol polyphosphate 5-phosphatase OCRL-1
UniProt Synonym Protein Names
Lowe oculocerebrorenal syndrome protein
Protein Family
Inositol polyphosphate 5-phosphatase
UniProt Gene Name
OCRL
UniProt Synonym Gene Names
INPP5F; OCRL1
UniProt Entry Name
OCRL_HUMAN
NCBI Protein Information
inositol polyphosphate 5-phosphatase OCRL-1; Lowe oculocerebrorenal syndrome protein; phosphatidylinositol polyphosphate 5-phosphatase

NCBI Summary for OCRL

This gene encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. [provided by RefSeq, Jul 2008]

UniProt Comments for OCRL

OCRL: Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5- trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5- tetrakisphosphate to inositol 1,3,4-trisphosphate. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. Belongs to the inositol-1,4,5-trisphosphate 5- phosphatase type II family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.36; Phosphatase (non-protein); Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: phagocytic vesicle membrane; Golgi stack; photoreceptor outer segment; clathrin-coated vesicle; early endosome membrane; early endosome; cytoplasm; plasma membrane; Golgi-associated vesicle; coated pit; trans-Golgi network; cytosol; nucleus

Molecular Function: protein binding; phosphoinositide 5-phosphatase activity; Rac GTPase binding

Biological Process: regulation of small GTPase mediated signal transduction; inositol phosphate metabolic process; in utero embryonic development; phospholipid metabolic process; small GTPase mediated signal transduction; phosphatidylinositol biosynthetic process; phosphoinositide dephosphorylation; cilium biogenesis; lipid metabolic process

Disease: Lowe Oculocerebrorenal Syndrome; Dent Disease 2
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Research Articles on OCRL

1. Implications of OCRL and TRPV4 in primary cilia function may also shed light on mechanosensation in other organ systems.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-OCRL antibody

 Products by Pathway  Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 Clathrin Derived Vesicle Budding Pathway antibodies  Clathrin Derived Vesicle Budding Pathway Diagram
 D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Degradation Pathway Diagram
 Golgi Associated Vesicle Biogenesis Pathway antibodies  Golgi Associated Vesicle Biogenesis Pathway Diagram

Diseases associated with anti-OCRL antibody

 Disease Name  Pubmed Publications
 Oculocerebrorenal Syndrome Antibodies  >145 publications with OCRL and Oculocerebrorenal Syndrome
 Kidney Diseases Antibodies  >134 publications with OCRL and Kidney Diseases
 Nervous System Diseases Antibodies  >124 publications with OCRL and Nervous System Diseases
 Proteinuria Antibodies  >23 publications with OCRL and Proteinuria
 Dent Disease 2 Antibodies  >8 publications with OCRL and Dent Disease 2
 Disease Models, Animal Antibodies  >6 publications with OCRL and Disease Models, Animal
 Neurobehavioral Manifestations Antibodies  >6 publications with OCRL and Neurobehavioral Manifestations
 Developmental Disabilities Antibodies  >4 publications with OCRL and Developmental Disabilities
 Dent disease 1 Antibodies  >4 publications with OCRL and Dent disease 1
 Weight Loss Antibodies  >3 publications with OCRL and Weight Loss

Organs/Tissues associated with anti-OCRL antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >47 publications with OCRL and Kidney
 Brain Antibodies  >18 publications with OCRL and Brain
 Eye Antibodies  >15 publications with OCRL and Eye
 Muscle Antibodies  >9 publications with OCRL and Muscle
 Bone Antibodies  >7 publications with OCRL and Bone
 Skin Antibodies  >6 publications with OCRL and Skin
 Intestine Antibodies  >2 publications with OCRL and Intestine
 Embryonic Tissue Antibodies  >2 publications with OCRL and Embryonic Tissue
 Liver Antibodies  >2 publications with OCRL and Liver
 Testis Antibodies  >2 publications with OCRL and Testis
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