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PRAGMIN Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245772

MBS245772 is an antibody targeting PRAGMIN. Also known as Mouse Monoclonal [clone 5C6] (IgG2a,k) to Human PRAGMIN / SGK223. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PRAGMIN / SGK223, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 5C6] (IgG2a,k) to Human PRAGMIN / SGK223
Product Gene Name
Product Synonym Gene Name
SGK223
Product Synonym Names
Anti-PRAGMIN / SGK223 Antibody (clone 5C6) IHC-plus; SGK223; Homolog of rat pragma of Rnd2; PRAGMIN; Sugen kinase 223; Tyrosine-protein kinase SgK223; Human PRAGMIN; SGK223
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
5C6
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.4 mg/ml (lot specific)
Specificity
Human PRAGMIN
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
149,624 Da
3D Structure
ModBase 3D Structure for Q86YV5
Target Species
Human
Immunogen Description
DKFZp761P0423 (XP_291277, 2 a.a. ~ 102 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
PRAGMIN / SGK223 antibody was raised against dKFZp761P0423 (XP_291277, 2 a.a. ~ 102 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-PRAGMIN antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PRAGMIN antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot with the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-PRAGMIN antibody

Anti-SGK223 / PRAGMIN antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-PRAGMIN Monoclonal Antibody – Cat# MBS245772

Other Notes

Small volumes of anti-PRAGMIN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRAGMIN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tyrosine-protein kinase SgK223
NCBI Official Symbol
SGK223
NCBI Official Synonym Symbols
PRAGMIN
UniProt Protein Name
Tyrosine-protein kinase SgK223
UniProt Synonym Protein Names
Sugen kinase 223
UniProt Gene Name
SGK223
UniProt Entry Name
SG223_HUMAN
NCBI Protein Information
tyrosine-protein kinase SgK223; sugen kinase 223

NCBI Summary for PRAGMIN

This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA). [provided by RefSeq, Mar 2014]

UniProt Comments for PRAGMIN

SGK223: a protein kinase of the NKF3 family.

Protein type: Protein kinase, Other; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Motility/polarity/chemotaxis; Other group; NKF3 family

Chromosomal Location of Human Ortholog: 8p23.1

Molecular Function: non-membrane spanning protein tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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