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MARCKSL1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245795

MBS245795 is an antibody targeting MARCKSL1. Also known as Mouse Monoclonal [clone 8C8] (IgG2b,k) to Human MARCKSL1 / MRP. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MARCKSL1 / MRP, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 8C8] (IgG2b,k) to Human MARCKSL1 / MRP
Product Gene Name
Product Synonym Gene Name
MRP
Product Synonym Names
Anti-MARCKSL1 / MRP Antibody (clone 8C8) IHC-plus; MARCKSL1; F52; MARCKS-like protein; MACMARCKS; MLP1; Mac-MARCKS; MARCKS-like 1; MARCKS-like protein 1; MRP; MARCKS-related protein; MLP; Human MARCKSL1; MRP
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
8C8
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2
Purity/Purification
Protein A Purified
Concentration
0.22 mg/ml (lot specific)
Specificity
Human Marcks-Related Protein
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
19,529 Da
OMIM
602940
3D Structure
ModBase 3D Structure for P49006
Target Species
Human
Immunogen Description
MARCKSL1 (NP_075385, 107 a.a. ~ 196 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
MARCKSL1 / MRP antibody was raised against mARCKSL1 (NP_075385, 107 a.a. ~ 196 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-MARCKSL1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-MARCKSL1 antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates and the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-MARCKSL1 antibody

Anti-MARCKSL1 / MRP antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-MARCKSL1 Monoclonal Antibody – Cat# MBS245795

Western Blot (WB) of anti-MARCKSL1 antibody

Western blot of MARCKSL1 expression in transfected 293T cell line by antibody.
Mouse Anti-MARCKSL1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245795

ELISA (EIA) of anti-MARCKSL1 antibody

Detection limit for recombinant GST tagged MARCKSL1 is approximately 0.1 ng/ml as a capture antibody.
Mouse Anti-MARCKSL1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS245795

Other Notes

Small volumes of anti-MARCKSL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MARCKSL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
MARCKS-related protein
NCBI Official Synonym Full Names
MARCKS-like 1
NCBI Official Symbol
MARCKSL1
NCBI Official Synonym Symbols
F52; MLP; MRP; MLP1; MACMARCKS
UniProt Protein Name
MARCKS-related protein
UniProt Synonym Protein Names
MARCKS-like protein 1; Macrophage myristoylated alanine-rich C kinase substrate; Mac-MARCKS; MacMARCKS
UniProt Gene Name
MARCKSL1
UniProt Synonym Gene Names
MLP; MRP; Mac-MARCKS; MacMARCKS
UniProt Entry Name
MRP_HUMAN
NCBI Protein Information
MARCKS-related protein; mac-MARCKS; MARCKS-like protein 1; macrophage myristoylated alanine-rich C kinase substrate

NCBI Summary for MARCKSL1

This gene encodes a member of the myristoylated alanine-rich C-kinase substrate (MARCKS) family. Members of this family play a role in cytoskeletal regulation, protein kinase C signaling and calmodulin signaling. The encoded protein affects the formation of adherens junction. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on the long arm of chromosomes 6 and 10. [provided by RefSeq, Jun 2012]
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UniProt Comments for MARCKSL1

MLP: Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration. When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration. May also affect cancer cell migration. May be involved in coupling the protein kinase C and calmodulin signal transduction systems. Belongs to the MARCKS family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: cytoplasm; plasma membrane

Molecular Function: calmodulin binding; actin binding

Biological Process: positive regulation of cell proliferation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MARCKSL1 antibody

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