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Class I Free Chain (HLA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245797

MBS245797 is an antibody targeting HLA Class I Free Chain. Also known as Mouse Monoclonal [clone A4] (IgG1) to Human HLA Class I Free Chain. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
HLA Class I Free Chain, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone A4] (IgG1) to Human HLA Class I Free Chain
Product Synonym Names
Anti-HLA Class I Free Chain Antibody (clone A4) IHC-plus; HLA Class I Free Chain; Human HLA Class I Free Chain
Clonality
Monoclonal
Isotype
IgG1
Clone Number
A4
Host
Mouse
Species Reactivity
Human
Form/Format
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Purity/Purification
Affinity Purified
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C, avoid repeated freeze thaw cycles.
Target Species
Human
Immunogen Description
Human HLA- MHC Class I Free Chain
Immunogen
Human HLA- MHC Class I Free Chain
Applications Tested/Suitable for anti-HLA Class I Free Chain antibody
Immunohistochemistry (IHC - Paraffin), Flow Cytometry (FC/FACS)
Application Notes for anti-HLA Class I Free Chain antibody
Flo, IHC-P (20 ug/ml)
Usage: This A4 antibody has been tested by flow cytometric analysis of 3-day PHA-activated PBMC. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 1...

Immunohistochemistry (IHC) of anti-HLA Class I Free Chain antibody

Anti-HLA-MHC Class I Free Chain antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Mouse Anti-HLA Monoclonal Antibody – Cat# MBS245797

Immunohistochemistry (IHC) of anti-HLA Class I Free Chain antibody

Anti-HLA-MHC Class I Free Chain antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Mouse Anti-HLA Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245797

Flow Cytometry (FC/FACS) of anti-HLA Class I Free Chain antibody

Staining of mononuclear cells 3-day PHA-activated normal human peripheral blood with 0.25 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.25 ug of Purified anti-human HLA- MHC Class I free chain without B2m (A4) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L, Minimal Reactivity to Rat IgG). Total viable cells were used for analysis.
Mouse Anti-HLA Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS245797

Flow Cytometry (FC/FACS) of anti-HLA Class I Free Chain antibody

Staining of normal human peripheral blood mononuclear cells either unactivated with 0.25 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.25 ug of Purified anti-human HLA- MHC Class I free chain without B2m (A4) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L, Minimal Reactivity to Rat IgG). Total viable cells were used for analysis.
Mouse Anti-HLA Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS245797

Other Notes

Small volumes of anti-HLA Class I Free Chain antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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