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EN1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245826

MBS245826 is an antibody targeting EN1. Also known as Mouse Monoclonal [clone 3H2] (IgG2a,k) to Human EN1 / Engrailed. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
EN1 / Engrailed, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 3H2] (IgG2a,k) to Human EN1 / Engrailed
Product Gene Name
anti-EN1 antibody
Product Synonym Names
Anti-EN1 / Engrailed Antibody (clone 3H2) IHC-plus; EN1; Engrailed 1; Engrailed homolog 1; Homeobox protein en-1; Homeobox protein engrailed-1; Hu-En-1; Engrailed homeobox 1; Human EN1; Engrailed
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3H2
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human Engrailed Homolog 1
Preparation and Storage
Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.
Molecular Weight
40,115 Da
OMIM
131290
3D Structure
ModBase 3D Structure for Q05925
Target Species
Human
Immunogen Description
EN1 (NP_001417, 266 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
EN1 / Engrailed antibody was raised against eN1 (NP_001417, 266 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-EN1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-EN1 antibody
ELISA, IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western blot using the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-EN1 antibody

Anti-EN1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-EN1 Monoclonal Antibody – Cat# MBS245826

ELISA (EIA) of anti-EN1 antibody

Detection limit for recombinant GST tagged EN1 is approximately 1 ng/ml as a capture antibody.
Mouse Anti-EN1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS245826

Other Notes

Small volumes of anti-EN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EN1. It may not necessarily be applicable to this product.

NCBI GI #
126090909
NCBI GeneID
2019
NCBI Accession #
NP_001417.3
UniProt Primary Accession #
Q05925
UniProt Secondary Accession #
Q4ZG44
UniProt Related Accession #
Q05925
GenBank Nucleotide #
NM_001426.3
NCBI Official Full Name
homeobox protein engrailed-1
NCBI Official Synonym Full Names
engrailed homeobox 1
NCBI Official Symbol
EN1
UniProt Protein Name
Homeobox protein engrailed-1
Protein Family
Homeobox protein
UniProt Gene Name
EN1
UniProt Synonym Gene Names
Homeobox protein en-1; Hu-En-1
UniProt Entry Name
HME1_HUMAN
NCBI Protein Information
homeobox protein engrailed-1; hu-En-1; engrailed homolog 1; homeobox protein en-1

NCBI Summary for EN1

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]
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UniProt Comments for EN1

EN1: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]

Protein type: Transcription factor; Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: membrane; nucleus

Biological Process: embryonic forelimb morphogenesis; anatomical structure morphogenesis; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; dorsal/ventral pattern formation; pigmentation; midbrain-hindbrain boundary development; midbrain development; neuron development; positive regulation of transcription from RNA polymerase II promoter; skeletal development; proximal/distal pattern formation; hindbrain development
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Research Articles on EN1

1. EN1 is an activator of intrinsic inflammatory pathways associated with prosurvival in basal-like breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EN1 antibody

 Reference Product  PubMed Publications
 EN2 antibody  >50 publications with EN1 and EN2
 FGF8 antibody  >47 publications with EN1 and FGF8
 PAX2 antibody  >32 publications with EN1 and PAX2
 SHH antibody  >27 publications with EN1 and SHH
 JUN antibody  >23 publications with EN1 and JUN
 PAX6 antibody  >18 publications with EN1 and PAX6
 FGF4 antibody  >5 publications with EN1 and FGF4
 HPRT1 antibody  >2 publications with EN1 and HPRT1

Pathways associated with anti-EN1 antibody

 Products by Pathway  Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram

Diseases associated with anti-EN1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >47 publications with EN1 and Nervous System Diseases
 Brain Diseases Antibodies  >25 publications with EN1 and Brain Diseases
 Neurodegenerative Diseases Antibodies  >24 publications with EN1 and Neurodegenerative Diseases
 Central Nervous System Diseases Antibodies  >24 publications with EN1 and Central Nervous System Diseases
 Movement Disorders Antibodies  >16 publications with EN1 and Movement Disorders
 Parkinson Disease Antibodies  >16 publications with EN1 and Parkinson Disease
 Parkinsonian Disorders Antibodies  >14 publications with EN1 and Parkinsonian Disorders
 Disease Models, Animal Antibodies  >14 publications with EN1 and Disease Models, Animal
 Neurobehavioral Manifestations Antibodies  >4 publications with EN1 and Neurobehavioral Manifestations
 Nerve Degeneration Antibodies  >3 publications with EN1 and Nerve Degeneration

Organs/Tissues associated with anti-EN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >78 publications with EN1 and Embryonic Tissue
 Bone Antibodies  >35 publications with EN1 and Bone
 Placenta Antibodies  >2 publications with EN1 and Placenta
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