• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

RAPGEF6 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245856

MBS245856 is an antibody targeting RAPGEF6. Also known as Mouse Monoclonal [clone 2C5] (IgG2a,k) to Human RAPGEF6. Reactive in Human. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
RAPGEF6, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2C5] (IgG2a,k) to Human RAPGEF6
Product Gene Name
Product Synonym Names
Anti-RAPGEF6 Antibody (clone 2C5) IHC-plus; RAPGEF6; PDZGEF2; KIA001LB; RA-GEF-2; PDZ-GEF2; RAGEF2; Human RAPGEF6
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2C5
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human RAPGEF6
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
93,442 Da
OMIM
610499
Target Species
Human
Immunogen Description
RAPGEF6 (NP_057424, 1012 a.a. ~ 1111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
RAPGEF6 antibody was raised against rAPGEF6 (NP_057424, 1012 a.a. ~ 1111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-RAPGEF6 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RAPGEF6 antibody
ELISA, IF (40 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot using cell line lysates and the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-RAPGEF6 antibody

Anti-RAPGEF6 / RAGEF2 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-RAPGEF6 Monoclonal Antibody – Cat# MBS245856

Immunofluorescence (IF) of anti-RAPGEF6 antibody

Immunofluorescence of monoclonal antibody to RAPGEF6 on HeLa cell. [antibody concentration 40 ug/ml]
Mouse Anti-RAPGEF6 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS245856

Western Blot (WB) of anti-RAPGEF6 antibody

in Western blot of RAPGEF6 expression in HeLa NE.
Mouse Anti-RAPGEF6 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245856

Other Notes

Small volumes of anti-RAPGEF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RAPGEF6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens chromosome 5, GRCh37.p13 Primary Assembly
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor (GEF) 6
NCBI Official Symbol
RAPGEF6
NCBI Official Synonym Symbols
RAGEF2; PDZGEF2; KIA001LB; PDZ-GEF2; RA-GEF-2
UniProt Protein Name
Rap guanine nucleotide exchange factor 6
UniProt Synonym Protein Names
PDZ domain-containing guanine nucleotide exchange factor 2; PDZ-GEF2; RA-GEF-2
UniProt Gene Name
RAPGEF6
UniProt Synonym Gene Names
PDZGEF2; PDZ-GEF2
UniProt Entry Name
RPGF6_HUMAN
NCBI Protein Information
rap guanine nucleotide exchange factor 6; PDZ domain-containing guanine nucleotide exchange factor 2; PDZ domain-containing guanine nucleotide exchange factor I; PDZ domain containing guanine nucleotide exchange factor (GEF) 2

UniProt Comments for RAPGEF6

RAPGEF6: Guanine nucleotide exchange factor (GEF) for Rap1A, Rap2A and M-Ras GTPases. Does not interact with cAMP. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Ras; GEFs

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: endocytic vesicle; apical plasma membrane; plasma membrane; cytosol

Molecular Function: protein binding; Ras GTPase binding; GTP-dependent protein binding; guanyl-nucleotide exchange factor activity

Biological Process: microvillus biogenesis; regulation of GTPase activity; Ras protein signal transduction
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000