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CCL19 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245857

May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to… More details

$650 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CCL19 / MIP3-Beta, Monoclonal Antibody
Full Product Name
Mouse Monoclonal (IgG1,k) to Human CCL19 / MIP3-Beta
Product Gene Name
anti-CCL19 antibody
Product Synonym Names
Anti-CCL19 / MIP3-Beta Antibody IHC-plus; CCL19; C-C motif chemokine 19; CKb11; CC chemokine ligand 19; EBI1 ligand chemokine; EBI1-ligand chemokine; Exodus-3; ELC; MIP-3-beta; MIP3B; SCYA19; Small-inducible cytokine A19; Beta chemokine exodus-3; Beta-chemokine exodus-3; CK beta-11; MIP-3b; MIP3-Beta; Human CCL19; MIP3-Beta
Clonality
Monoclonal
Isotype
IgG1,k
Host
Mouse
Species Reactivity
Human
Form/Format
Lyophilized from PBS
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human MIP-3beta
Preparation and Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8 degree C. Aliquot and freeze at -20 degree C for long term storage. Avoid freeze/thaw cycles.
OMIM
602227
3D Structure
ModBase 3D Structure for Q99731
Target
CCL19/MIP3-Beta
Target Species
Human
Immunogen Species
CCL19/MIP3-Beta antibody was raised against Human
Immunogen Type
Cell extract
Immunogen
CCL19 / MIP3-Beta antibody was raised against e.coli derived recombinant Human MIP-3beta.
Reconstitution
Reconstitute with sterile water.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CCL19 antibody

Family: Intercrine; Subfamily: Intercrine beta
Applications Tested/Suitable for anti-CCL19 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-CCL19 antibody
IHC - Paraffin (10 µg/ml)
Western blot (1 - 2 µg/ml)
ELISA (2 - 4 µg/ml)

Immunohistochemistry:
MBS245857 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended.
ELISA:
In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant Human MIP-3beta when used with biotinylated antigen affinity purified anti-Human MIP-3beta as the detection antibody at a concentration of approximately 0.5-1 ug/ml.
Western Blot:
To detect Human MIP-3beta by Western Blot analysis this antibody can be used at a concentration of 1-2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3beta is 2-4 ng/lane, under reducing conditions.

Immunohistochemistry (IHC) of anti-CCL19 antibody

Anti-CCL19 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Mouse Anti-CCL19 Monoclonal Antibody – Cat# MBS245857

Other Notes

Small volumes of anti-CCL19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL19 purchase

MBS245857 is an antibody targeting CCL19. Also known as Mouse Monoclonal (IgG1,k) to Human CCL19 / MIP3-Beta. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL19. It may not necessarily be applicable to this product.

NCBI GI #
5454040
NCBI GeneID
6363
NCBI Accession #
NP_006265.1
UniProt Primary Accession #
Q99731
UniProt Secondary Accession #
O00697; O00736
UniProt Related Accession #
Q99731
GenBank Nucleotide #
NM_006274.2
NCBI Official Full Name
C-C motif chemokine 19
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 19
NCBI Official Symbol
CCL19
NCBI Official Synonym Symbols
ELC; CKb11; MIP3B; MIP-3b; SCYA19
UniProt Protein Name
C-C motif chemokine 19
UniProt Synonym Protein Names
Beta-chemokine exodus-3; CK beta-11; Epstein-Barr virus-induced molecule 1 ligand chemokine; EBI1 ligand chemokine; ELC; Macrophage inflammatory protein 3 beta; MIP-3-beta; Small-inducible cytokine A19
Protein Family
C-C motif chemokine
UniProt Gene Name
CCL19
UniProt Synonym Gene Names
ELC; MIP3B; SCYA19; EBI1 ligand chemokine; ELC; MIP-3-beta
UniProt Entry Name
CCL19_HUMAN
NCBI Protein Information
C-C motif chemokine 19; exodus-3; CK beta-11; MIP-3-beta; EBI1 ligand chemokine; EBI1-ligand chemokine; CC chemokine ligand 19; beta chemokine exodus-3; beta-chemokine exodus-3; small-inducible cytokine A19; macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; epstein-Barr virus-induced molecule 1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19

NCBI Summary for CCL19

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Sep 2014]
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UniProt Comments for CCL19

CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: extracellular space; extracellular region

Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; CCR7 chemokine receptor binding

Biological Process: formation of immunological synapse; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of endocytosis; positive regulation of interleukin-1 beta secretion; activation of JNK activity; positive regulation of receptor-mediated endocytosis; dendritic cell chemotaxis; establishment of T cell polarity; positive regulation of T cell proliferation; cell communication; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of chemotaxis; response to virus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tumor necrosis factor production; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; T cell costimulation; immune response; positive regulation of T-helper 1 cell differentiation
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Research Articles on CCL19

1. Serum CCL19 and CCL21 were up-regulated during Rickettsia conorii infection.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CCL19 antibody

 Reference Product  PubMed Publications
 CCR7 antibody  >438 publications with CCL19 and CCR7
 CXCL13 antibody  >98 publications with CCL19 and CXCL13
 CXCL10 antibody  >55 publications with CCL19 and CXCL10
 CCR5 antibody  >50 publications with CCL19 and CCR5
 CCR6 antibody  >47 publications with CCL19 and CCR6
 CXCL9 antibody  >36 publications with CCL19 and CXCL9
 CXCR5 antibody  >31 publications with CCL19 and CXCR5
 CCL25 antibody  >30 publications with CCL19 and CCL25
 CCR1 antibody  >23 publications with CCL19 and CCR1
 CXCL11 antibody  >21 publications with CCL19 and CXCL11

Pathways associated with anti-CCL19 antibody

 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram

Diseases associated with anti-CCL19 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >149 publications with CCL19 and Inflammation
 Necrosis Antibodies  >66 publications with CCL19 and Necrosis
 Respiratory Tract Diseases Antibodies  >48 publications with CCL19 and Respiratory Tract Diseases
 Lung Diseases Antibodies  >44 publications with CCL19 and Lung Diseases
 Vascular Diseases Antibodies  >31 publications with CCL19 and Vascular Diseases
 Hypersensitivity Antibodies  >27 publications with CCL19 and Hypersensitivity
 Death Antibodies  >21 publications with CCL19 and Death
 Lung Neoplasms Antibodies  >14 publications with CCL19 and Lung Neoplasms
 Respiratory Tract Infections Antibodies  >14 publications with CCL19 and Respiratory Tract Infections
 Liver Diseases Antibodies  >13 publications with CCL19 and Liver Diseases

Organs/Tissues associated with anti-CCL19 antibody

 Organ/Tissue Name  Pubmed Publications
 Lymph Node Antibodies  >279 publications with CCL19 and Lymph Node
 Vascular Antibodies  >84 publications with CCL19 and Vascular
 Spleen Antibodies  >70 publications with CCL19 and Spleen
 Lung Antibodies  >64 publications with CCL19 and Lung
 Thymus Antibodies  >38 publications with CCL19 and Thymus
 Brain Antibodies  >30 publications with CCL19 and Brain
 Intestine Antibodies  >23 publications with CCL19 and Intestine
 Eye Antibodies  >19 publications with CCL19 and Eye
 Pancreas Antibodies  >17 publications with CCL19 and Pancreas
 Heart Antibodies  >14 publications with CCL19 and Heart
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