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GFAP Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438192

MBS438192 is an antibody targeting GFAP. Also known as GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GFAP, Monoclonal Antibody
Full Product Name
GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody
Product Gene Name
Product Synonym Names
Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM507
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken
Form/Format
Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.
Purity/Purification
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
Specificity
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Preparation and Storage
Antibody with azide , store at 2°C to 8°C
Non hazardous
No MSDS
Molecular Weight
~50kDa
OMIM
137780
3D Structure
ModBase 3D Structure for P14136
Cellular Localization
Cytoplasmic
Immunogen
GFAP isolated from pig spinal cord
Positive Control
T98G cells (FC), Brain Iysate (WB),Brain or Astrocytoma (IHC)
Human Chromosome Location
17q21.31
Applications Tested/Suitable for anti-GFAP antibody
Flow Cytometry (FC), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-GFAP antibody
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-GFAP antibody

Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Monoclonal Antibody (SPM507).
Mouse Anti-GFAP Monoclonal Antibody – Cat# MBS438192

Western Blot (WB) of anti-GFAP antibody

Western Blot Analysis of Human Brain Tissue Lysate using GFAP Mab (SPM507).
Mouse Anti-GFAP Monoclonal Antibody, Western Blot (WB) – Cat# MBS438192

SDS-PAGE of anti-GFAP antibody

SDS-PAGE Analysis
Purified EGFR Mouse Monoclonal Antibody (GFR/2596).
Confirmation of Integrity and Purity of Antibody
Mouse Anti-GFAP Monoclonal Antibody, SDS-PAGE – Cat# MBS438192

Product References and Citations for anti-GFAP antibody

Herpers MJ et. Al. 1986, Acta Neuropathol, 70:333-339. Van Muijen GN et. al. 1987, Lab Invest, 57:359-369. Debus E, et. al. 1983, Differentiation, 25(2):193-203

Other Notes

Small volumes of anti-GFAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GFAP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glial fibrillary acidic protein isoform 2
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
GFAP
NCBI Official Synonym Symbols
ALXDRD
UniProt Protein Name
Glial fibrillary acidic protein
UniProt Gene Name
GFAP
UniProt Synonym Gene Names
GFAP
UniProt Entry Name
GFAP_HUMAN
NCBI Protein Information
glial fibrillary acidic protein

NCBI Summary for GFAP

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
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UniProt Comments for GFAP

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; cytosol; intermediate filament cytoskeleton; lysosome

Molecular Function: protein binding

Biological Process: regulation of protein complex assembly

Disease: Alexander Disease
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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