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TOMM22 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245944

MBS245944 is an antibody targeting TOMM22. Also known as Mouse Monoclonal [clone 4G4] (IgG2a,k) to Human TOMM22 / TOM22. Reactive in Human, Mouse, Rat. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TOMM22 / TOM22, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 4G4] (IgG2a,k) to Human TOMM22 / TOM22
Product Gene Name
anti-TOMM22 antibody
Product Synonym Gene Name
TOM22
Product Synonym Names
Anti-TOMM22 / TOM22 Antibody (clone 4G4) IHC-plus; TOMM22; 1C9-2; MSTP065; MST065; HTom22; TOM22; Human TOMM22; TOM22
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4G4
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human TOMM22
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
15,522 Da
OMIM
607046
3D Structure
ModBase 3D Structure for Q9NS69
Target Species
Human
Immunogen Description
TOMM22 (AAH09363, 1 a.a. ~ 143 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
TOMM22 / TOM22 antibody was raised against tOMM22 (AAH09363, 1 a.a. ~ 143 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-TOMM22 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-TOMM22 antibody
ELISA, IF (10 ug/ml), IHC-P (10 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-TOMM22 antibody

Anti-TOMM22 antibody IHC of human liver, hepatocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-TOMM22 Monoclonal Antibody – Cat# MBS245944

Immunofluorescence (IF) of anti-TOMM22 antibody

Immunofluorescence of monoclonal antibody to TOMM22 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-TOMM22 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS245944

Western Blot (WB) of anti-TOMM22 antibody

Western blot of TOMM22 expression in Raw 264.7.
Mouse Anti-TOMM22 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245944

Western Blot (WB) of anti-TOMM22 antibody

Western blot of TOMM22 expression in NIH/3T3.
Mouse Anti-TOMM22 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245944

Western Blot (WB) of anti-TOMM22 antibody

Western blot of TOMM22 expression in PC-12.
Mouse Anti-TOMM22 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245944

ELISA (EIA) of anti-TOMM22 antibody

Detection limit for recombinant GST tagged TOMM22 is 0.1 ng/ml as a capture antibody.
Mouse Anti-TOMM22 Monoclonal Antibody, ELISA (EIA) – Cat# MBS245944

Other Notes

Small volumes of anti-TOMM22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TOMM22. It may not necessarily be applicable to this product.

NCBI GI #
9910382
NCBI GeneID
56993
NCBI Accession #
NP_064628.1
UniProt Primary Accession #
Q9NS69
UniProt Related Accession #
Q9NS69
GenBank Nucleotide #
NM_020243.4
NCBI Official Full Name
mitochondrial import receptor subunit TOM22 homolog
NCBI Official Synonym Full Names
translocase of outer mitochondrial membrane 22 homolog (yeast)
NCBI Official Symbol
TOMM22
NCBI Official Synonym Symbols
1C9-2; TOM22; MST065; MSTP065
UniProt Protein Name
Mitochondrial import receptor subunit TOM22 homolog
UniProt Synonym Protein Names
1C9-2; Translocase of outer membrane 22 kDa subunit homolog
UniProt Gene Name
TOMM22
UniProt Synonym Gene Names
TOM22; hTom22
UniProt Entry Name
TOM22_HUMAN
NCBI Protein Information
mitochondrial import receptor subunit TOM22 homolog; mitochondrial import receptor Tom22; translocase of outer membrane 22 kDa subunit homolog

NCBI Summary for TOMM22

The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion. [provided by RefSeq, Jul 2008]

UniProt Comments for TOMM22

TOMM22: Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Belongs to the Tom22 family.

Protein type: Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: mitochondrial outer membrane translocase complex; membrane; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: protein binding; protein transmembrane transporter activity

Biological Process: cellular protein metabolic process; protein import into mitochondrial outer membrane; protein targeting to mitochondrion
Show more

Research Articles on TOMM22

1. Blockade of mitochondrial protein import triggers the recruitment of PARK2, by PINK1, to the TOMM machinery.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TOMM22 antibody

 Reference Product  PubMed Publications
 TOMM20 antibody  >7 publications with TOMM22 and TOMM20
 TOMM40 antibody  >2 publications with TOMM22 and TOMM40
 TOMM70A antibody  >1 publications with TOMM22 and TOMM70A

Pathways associated with anti-TOMM22 antibody

 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Mitochondrial Protein Import Pathway antibodies  Mitochondrial Protein Import Pathway Diagram

Diseases associated with anti-TOMM22 antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >1 publications with TOMM22 and Cell Transformation, Neoplastic
 Death Antibodies  >1 publications with TOMM22 and Death
 Liver Diseases Antibodies  >1 publications with TOMM22 and Liver Diseases
 Carcinoma Antibodies  >1 publications with TOMM22 and Carcinoma

Organs/Tissues associated with anti-TOMM22 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >5 publications with TOMM22 and Liver
 Prostate Antibodies  >1 publications with TOMM22 and Prostate
 Embryonic Tissue Antibodies  >1 publications with TOMM22 and Embryonic Tissue
 Muscle Antibodies  >1 publications with TOMM22 and Muscle
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