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BAG1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245958

MBS245958 is an antibody targeting BAG1. Also known as Mouse Monoclonal [clone 2D3] (IgG2a,k) to Human BAG1 / BAG-1. Reactive in Human, Mouse, Rat. Host: Mouse. More details

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Product Overview

Product Name
BAG1 / BAG-1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2D3] (IgG2a,k) to Human BAG1 / BAG-1
Product Gene Name
anti-BAG1 antibody
Product Synonym Gene Name
BAG-1
Product Synonym Names
Anti-BAG1 / BAG-1 Antibody (clone 2D3) IHC-plus; BAG1; Bcl-2-associated athanogene 1; Bcl-2-binding protein; Bcl2 binding protein bag1; BCL2-associated athanogene; BAG-1; Bcl2 binding athanogene1; HAP; RAP46; Human BAG1; BAG-1
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2D3
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human Bag1
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
25,989 Da
OMIM
601497
Target Species
Human
Immunogen Description
BAG1 (NP_004314, 241 a.a. ~ 346 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
BAG1 / BAG-1 antibody was raised against bAG1 (NP_004314, 241 a.a. ~ 346 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-BAG1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-BAG1 antibody
ELISA, IF (35 ug/ml), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Cell lysate, Cell lysate, Cell lysate, Cell lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-BAG1 antibody

Anti-BAG1 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-BAG1 Monoclonal Antibody – Cat# MBS245958

Immunofluorescence (IF) of anti-BAG1 antibody

Immunofluorescence of monoclonal antibody to BAG1 on HeLa cell. [antibody concentration 35 ug/ml]
Mouse Anti-BAG1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS245958

Western Blot (WB) of anti-BAG1 antibody

BAG1 monoclonal antibody (M02), clone 2D3. Western blot of BAG1 expression in NIH/3T3.
Mouse Anti-BAG1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245958

Western Blot (WB) of anti-BAG1 antibody

BAG1 monoclonal antibody (M02), clone 2D3 Western blot of BAG1 expression in HeLa.
Mouse Anti-BAG1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245958

Western Blot (WB) of anti-BAG1 antibody

BAG1 monoclonal antibody (M02), clone 2D3. Western blot of BAG1 expression in Jurkat.
Mouse Anti-BAG1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245958

Western Blot (WB) of anti-BAG1 antibody

BAG1 monoclonal antibody (M02), clone 2D3. Western blot of BAG1 expression in Raw 264.7.
Mouse Anti-BAG1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245958

ELISA (EIA) of anti-BAG1 antibody

Detection limit for recombinant GST tagged BAG1 is approximately 0.03 ng/ml as a capture antibody.
Mouse Anti-BAG1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS245958

Other Notes

Small volumes of anti-BAG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BAG1. It may not necessarily be applicable to this product.

NCBI GI #
224589821
NCBI GeneID
573
NCBI Accession #
NC_000009.11
UniProt Secondary Accession #
O75315; Q14414; Q53H32; Q5VZE8; Q5VZE9; Q5VZF0; Q96TG2; Q9Y2V4
UniProt Related Accession #
Q99933
GenBank Nucleotide #
NC_000009.11
NCBI Official Full Name
Homo sapiens chromosome 9, GRCh37.p13 Primary Assembly
NCBI Official Synonym Full Names
BCL2-associated athanogene
NCBI Official Symbol
BAG1
NCBI Official Synonym Symbols
HAP; BAG-1; RAP46
UniProt Protein Name
BAG family molecular chaperone regulator 1
UniProt Synonym Protein Names
Bcl-2-associated athanogene 1
Protein Family
BAG family molecular chaperone regulator
UniProt Gene Name
BAG1
UniProt Synonym Gene Names
HAP; BAG-1
UniProt Entry Name
BAG1_HUMAN
NCBI Protein Information
BAG family molecular chaperone regulator 1; Bcl-2-binding protein; receptor-associated protein, 46-KD; Bcl-2 associating athanogene-1 protein; glucocortoid receptor-associated protein RAP46

NCBI Summary for BAG1

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]
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UniProt Comments for BAG1

BAG1: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2. Up-regulated during differentiation of bladder epithelial cells and down-regulated during differentiation of prostate epithelium. Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9p12

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; chaperone binding; receptor signaling protein activity

Biological Process: cell surface receptor linked signal transduction; apoptosis; negative regulation of apoptosis
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Research Articles on BAG1

1. distinct isoforms of BAG-1 had different anti-apoptotic ability in breast cancer cells treated with the 4-OH TAM.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-BAG1 antibody

 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram

Diseases associated with anti-BAG1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >16 publications with BAG1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >10 publications with BAG1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >9 publications with BAG1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >4 publications with BAG1 and Cell Transformation, Neoplastic
 Adenocarcinoma Antibodies  >4 publications with BAG1 and Adenocarcinoma
 Nerve Degeneration Antibodies  >3 publications with BAG1 and Nerve Degeneration
 Prostatic Neoplasms Antibodies  >3 publications with BAG1 and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >2 publications with BAG1 and Neoplasms, Experimental
 Heart Diseases Antibodies  >2 publications with BAG1 and Heart Diseases
 Inflammation Antibodies  >2 publications with BAG1 and Inflammation

Organs/Tissues associated with anti-BAG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >126 publications with BAG1 and Muscle
 Nerve Antibodies  >49 publications with BAG1 and Nerve
 Brain Antibodies  >43 publications with BAG1 and Brain
 Blood Antibodies  >25 publications with BAG1 and Blood
 Embryonic Tissue Antibodies  >14 publications with BAG1 and Embryonic Tissue
 Heart Antibodies  >9 publications with BAG1 and Heart
 Eye Antibodies  >8 publications with BAG1 and Eye
 Lung Antibodies  >6 publications with BAG1 and Lung
 Liver Antibodies  >5 publications with BAG1 and Liver
 Prostate Antibodies  >4 publications with BAG1 and Prostate
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