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SGK1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245960

SGK, a SGK type protein kinase, is a multi-functional kinase that is phosphorylated and activated through a 3-phosphoinositide-dependent protein kinase 1 (PDK1) signaling pathway. The protein has been shown to activate certain potassium, sodium and chloride… More details

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Product Overview

Product Name
SGK1 / SGK, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1C4] (IgG2b,k) to Human SGK1 / SGK
Product Gene Name
anti-SGK1 antibody
Product Synonym Gene Name
SGK
Product Synonym Names
Anti-SGK1 / SGK Antibody (clone 1C4) IHC-plus; SGK1; Hsgk; Hsgk1; SGK; Sgk1 variant i3; Human SGK1; SGK
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1C4
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human SGK1
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
52,119 Da
OMIM
602958
3D Structure
ModBase 3D Structure for O00141
Target Species
Human
Immunogen Description
SGK (AAH01263, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
SGK1 / SGK antibody was raised against sGK (AAH01263, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Product Categories/Family for anti-SGK1 antibody

Family: Protein Kinase; Subfamily: SGK
Applications Tested/Suitable for anti-SGK1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SGK1 antibody
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot with the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-SGK1 antibody

Anti-SGK1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-SGK1 Monoclonal Antibody – Cat# MBS245960

Immunofluorescence (IF) of anti-SGK1 antibody

Immunofluorescence of monoclonal antibody to SGK1 on HeLa cell. [antibody concentration 10 ug/ml].
Mouse Anti-SGK1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS245960

ELISA (EIA) of anti-SGK1 antibody

Detection limit for recombinant GST tagged SGK is approximately 0.3 ng/ml as a capture antibody.
Mouse Anti-SGK1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS245960

Other Notes

Small volumes of anti-SGK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SGK1 purchase

MBS245960 is an antibody targeting SGK1. Also known as Mouse Monoclonal [clone 1C4] (IgG2b,k) to Human SGK1 / SGK. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SGK1. It may not necessarily be applicable to this product.

NCBI GI #
25168263
NCBI GeneID
6446
NCBI Accession #
NP_005618.2
UniProt Primary Accession #
O00141
UniProt Secondary Accession #
Q5TCN2; Q5TCN3; Q5TCN4; Q5VY65; Q9UN56; B7UUP7; B7UUP8; B7UUP9; B7Z5B2; E1P583
UniProt Related Accession #
O00141
GenBank Nucleotide #
NM_005627.3
NCBI Official Full Name
serine/threonine-protein kinase Sgk1 isoform 1
NCBI Official Synonym Full Names
serum/glucocorticoid regulated kinase 1
NCBI Official Symbol
SGK1
NCBI Official Synonym Symbols
SGK
UniProt Protein Name
Serine/threonine-protein kinase Sgk1
UniProt Synonym Protein Names
Serum/glucocorticoid-regulated kinase 1
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
SGK1
UniProt Synonym Gene Names
SGK
UniProt Entry Name
SGK1_HUMAN
NCBI Protein Information
serine/threonine-protein kinase Sgk1; Sgk1 variant i3; serine/threonine protein kinase SGK; serum/glucocorticoid-regulated kinase 1

NCBI Summary for SGK1

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]
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UniProt Comments for SGK1

SGK1: serum and glucocorticoid-inducible kinase is an AGC protein kinase of the SGK family. Rapidly induced in response to a variety of stimuli including serum, glucocorticoids, follicle stimulating hormone, osmotic shock and mineralocorticoids. Can be activated via PI3K-dependent and -independent pathways. Plays a role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. SGK is negatively regulated by ubiquitin modification and proteasome degradation.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; Motility/polarity/chemotaxis; AGC group; SGK family

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; mitochondrion; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; chloride channel regulator activity; potassium channel regulator activity; sodium channel regulator activity; calcium channel regulator activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: apoptosis; positive regulation of transporter activity; sodium ion transport; regulation of cell migration; protein amino acid phosphorylation; regulation of cell proliferation; regulation of apoptosis; regulation of transcription factor activity; long-term memory; regulation of catalytic activity; regulation of blood pressure; response to stress; regulation of cell growth; transmembrane transport; response to DNA damage stimulus
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Research Articles on SGK1

1. SGK1 selectively increases wild type-CFTR in the plasma membrane of human airway epithelia cells by inhibiting its endocytic retrieval from the membrane.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SGK1 antibody

 Products by Pathway  Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Class I PI3K Signaling Events Pathway antibodies  Class I PI3K Signaling Events Pathway Diagram
 FSH Signaling Pathway antibodies  FSH Signaling Pathway Diagram
 FoxO Family Signaling Pathway antibodies  FoxO Family Signaling Pathway Diagram
 FoxO Signaling Pathway antibodies  FoxO Signaling Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 IL-6 Signaling Pathway antibodies  IL-6 Signaling Pathway Diagram
 Insulin Pathway antibodies  Insulin Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram

Diseases associated with anti-SGK1 antibody

 Disease Name  Pubmed Publications
 Hypertension Antibodies  >80 publications with SGK1 and Hypertension
 Cardiovascular Diseases Antibodies  >51 publications with SGK1 and Cardiovascular Diseases
 Fibrosis Antibodies  >49 publications with SGK1 and Fibrosis
 Kidney Diseases Antibodies  >47 publications with SGK1 and Kidney Diseases
 Inflammation Antibodies  >33 publications with SGK1 and Inflammation
 Nervous System Diseases Antibodies  >25 publications with SGK1 and Nervous System Diseases
 Proteinuria Antibodies  >19 publications with SGK1 and Proteinuria
 Brain Diseases Antibodies  >14 publications with SGK1 and Brain Diseases
 Carcinoma Antibodies  >13 publications with SGK1 and Carcinoma
 Edema Antibodies  >12 publications with SGK1 and Edema

Organs/Tissues associated with anti-SGK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >206 publications with SGK1 and Kidney
 Blood Antibodies  >153 publications with SGK1 and Blood
 Brain Antibodies  >46 publications with SGK1 and Brain
 Heart Antibodies  >41 publications with SGK1 and Heart
 Lung Antibodies  >40 publications with SGK1 and Lung
 Muscle Antibodies  >39 publications with SGK1 and Muscle
 Intestine Antibodies  >23 publications with SGK1 and Intestine
 Liver Antibodies  >21 publications with SGK1 and Liver
 Vascular Antibodies  >19 publications with SGK1 and Vascular
 Bone Antibodies  >17 publications with SGK1 and Bone
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