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CDH18 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245968

MBS245968 is an antibody targeting CDH18. Also known as Mouse Monoclonal [clone 6F7] (IgG2a,k) to Human CDH18 / Ey-Cadherin. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CDH18 / Ey-Cadherin, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 6F7] (IgG2a,k) to Human CDH18 / Ey-Cadherin
Product Gene Name
Product Synonym Names
Anti-CDH18 / Ey-Cadherin Antibody (clone 6F7) IHC-plus; CDH18; Cadherin 14-like; Cadherin-14; CDH14L; Cadherin 18; type 2; Cadherin-18; CDH14; EY-CADHERIN; Cadherin-like 24; Human CDH18; Ey-Cadherin
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
6F7
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
63,677 Da
OMIM
603019
3D Structure
ModBase 3D Structure for Q13634
Target Species
Human
Immunogen Description
CDH18 (NP_004925, 467 a.a. ~ 577 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
CDH18 / Ey-Cadherin antibody was raised against cDH18 (NP_004925, 467 a.a. ~ 577 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Product Categories/Family for anti-CDH18 antibody

Applications Tested/Suitable for anti-CDH18 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CDH18 antibody
ELISA, IHC-P (5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control HeLa. Sandwich ELISA (Recombinant protein).

Immunohistochemistry (IHC) of anti-CDH18 antibody

Anti-CDH18 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-CDH18 Monoclonal Antibody – Cat# MBS245968

Immunohistochemistry (IHC) of anti-CDH18 antibody

Anti-CDH18 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-CDH18 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245968

Immunohistochemistry (IHC) of anti-CDH18 antibody

Anti-CDH18 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-CDH18 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS245968

Western Blot (WB) of anti-CDH18 antibody

CDH18 monoclonal antibody, clone 6F7 Western blot of CDH18 expression in HeLa.
Mouse Anti-CDH18 Monoclonal Antibody, Western Blot (WB) – Cat# MBS245968

ELISA (EIA) of anti-CDH18 antibody

Detection limit for recombinant GST tagged CDH18 is approximately 0.1 ng/ml as a capture antibody.
Mouse Anti-CDH18 Monoclonal Antibody, ELISA (EIA) – Cat# MBS245968

Other Notes

Small volumes of anti-CDH18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDH18. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cadherin-18 isoform 1 preproprotein
NCBI Official Synonym Full Names
cadherin 18, type 2
NCBI Official Symbol
CDH18
NCBI Official Synonym Symbols
CDH14; CDH24; CDH14L
UniProt Protein Name
Cadherin-18
UniProt Synonym Protein Names
Cadherin-14
Protein Family
UniProt Gene Name
CDH18
UniProt Synonym Gene Names
CDH14
UniProt Entry Name
CAD18_HUMAN
NCBI Protein Information
cadherin-18; cadherin-14; ey-cadherin; cadherin-like 24

NCBI Summary for CDH18

This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed specifically in the central nervous system and is putatively involved in synaptic adhesion, axon outgrowth and guidance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
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UniProt Comments for CDH18

CDH18: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 5p14.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: intercellular junction assembly and maintenance; homophilic cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-CDH18 antibody

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