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AMY1A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246000

MBS246000 is an antibody targeting AMY1A. Also known as Mouse Monoclonal [clone 2D4] (IgG2a,k) to Human AMY1A / Salivary Amylase. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
AMY1A / Salivary Amylase, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2D4] (IgG2a,k) to Human AMY1A / Salivary Amylase
Product Gene Name
Product Synonym Names
Anti-AMY1A / Salivary Amylase Antibody (clone 2D4) IHC-plus; Alpha-amylase 1; Glycogenase; AMY1; Amylase; alpha 1A (salivary); Amylase; alpha 1A salivary; Amylase; salivary; alpha-1A; Salivary amylase alpha 1A; Salivary alpha-amylase; Salivary Amylase; Human AMY1A; Salivary Amylase
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2D4
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.45 mg/ml (lot specific)
Specificity
Human AMY1A
Preparation and Storage
Aliquot and freeze at -20 degree C. Avoid freeze-thaw cycles.
Molecular Weight
57,768 Da
OMIM
gene 104702
3D Structure
ModBase 3D Structure for P04745
Target Species
Human
Immunogen Description
AMY1A (NP_001008222, 172 a.a. ~ 246 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
AMY1A / Salivary Amylase antibody was raised against aMY1A (NP_001008222, 172 a.a. ~ 246 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-AMY1A antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-AMY1A antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Tissue lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-AMY1A antibody

Anti-AMY1A / Salivary Amylase antibody IHC of human salivary gland. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-AMY1A Monoclonal Antibody – Cat# MBS246000

Western Blot (WB) of anti-AMY1A antibody

AMY1A monoclonal antibody, clone 2D4. Western blot of AMY1A expression in human pancreas.
Mouse Anti-AMY1A Monoclonal Antibody, Western Blot (WB) – Cat# MBS246000

Other Notes

Small volumes of anti-AMY1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AMY1A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-amylase 1
NCBI Official Synonym Full Names
amylase, alpha 1A (salivary)
NCBI Official Symbol
AMY1A
NCBI Official Synonym Symbols
AMY1
UniProt Protein Name
Alpha-amylase 1
UniProt Synonym Protein Names
1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase
UniProt Gene Name
AMY1A
UniProt Synonym Gene Names
AMY1
UniProt Entry Name
AMY1_HUMAN
NCBI Protein Information
alpha-amylase 1; glycogenase; salivary alpha-amylase; salivary amylase alpha 1A; amylase, salivary, alpha-1A; 1,4-alpha-D-glucan glucanohydrolase 1

NCBI Summary for AMY1A

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
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UniProt Comments for AMY1A

AMY1: Monomer. Belongs to the glycosyl hydrolase 13 family.

Protein type: Hydrolase; Secreted, signal peptide; Carbohydrate Metabolism - starch and sucrose; EC 3.2.1.1; Secreted

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular space

Molecular Function: alpha-amylase activity; protein binding; metal ion binding

Biological Process: digestion; carbohydrate metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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