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KHSRP Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS435128

The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization. More details

$435 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KHSRP, Monoclonal Antibody
Full Product Name
KHSRP Mouse mAb
Product Gene Name
Product Synonym Gene Name
6330409F21Rik; FBP2; FUBP2; KHSRP; KSRP; Marta1
Product Synonym Names
Far upstream element-binding protein 2; FBP2; FUBP2; FUSE binding protein 2; FUSE-binding protein 2; KH type-splicing regulatory protein; KH-type splicing regulatory protein; KSRP; MAP2 RNA trans-acting protein 1; MAP2 RNA trans-acting protein MARTA1; MARTA1; p75
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Molecular Weight
Predicted MW: 73kDa
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human FUBP2.
Applications Tested/Suitable for anti-KHSRP antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-KHSRP antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

Western Blot (WB) of anti-KHSRP antibody

All lanes : FUBP2 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 73 KDa Observed band size : 73 KDa Blocking/Dilution buffer : 1x TBST.
Mouse Anti-KHSRP Monoclonal Antibody – Cat# MBS435128

Immunoprecipitation (IP) of anti-KHSRP antibody

FUBP2 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25224 of 10 ug. Western blot was performed from the immunoprecipitate using M25224 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.

Blocking and dilution buffer and concentration:5% milk/TBST.
Mouse Anti-KHSRP Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS435128

Flow Cytometry (FC/FACS) of anti-KHSRP antibody

Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling FUBP2 with M25224 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
Mouse Anti-KHSRP Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS435128

Other Notes

Small volumes of anti-KHSRP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KHSRP purchase

MBS435128 is an antibody targeting KHSRP. Also known as KHSRP Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KHSRP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
KHSRP protein
NCBI Official Synonym Full Names
KH-type splicing regulatory protein
NCBI Official Symbol
KHSRP
NCBI Official Synonym Symbols
p75; FBP2; KSRP; FUBP2
UniProt Protein Name
Far upstream element-binding protein 2
UniProt Synonym Protein Names
KH type-splicing regulatory protein; KSRP; p75
UniProt Gene Name
KHSRP
UniProt Synonym Gene Names
FUBP2; FUSE-binding protein 2; KSRP
NCBI Protein Information
far upstream element-binding protein 2

NCBI Summary for KHSRP

The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization (Min et al., 1997 [PubMed 9136930]; Gherzi et al., 2004 [PubMed 15175153]).[supplied by OMIM, Apr 2010]

UniProt Comments for KHSRP

Binds to the dendritic targeting element and may play a role in mRNA trafficking (). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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