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THOC6 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246017

MBS246017 is an antibody targeting THOC6. Also known as Mouse Monoclonal [clone 1F6] (IgG1,k) to Human THOC6. Reactive in Human, Rat. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
THOC6, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1F6] (IgG1,k) to Human THOC6
Product Gene Name
anti-THOC6 antibody
Product Synonym Names
Anti-THOC6 Antibody (clone 1F6) IHC-plus; THOC6; FSAP35; WDR58; WD repeat domain 58; THO complex subunit 6 homolog; Human THOC6
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1F6
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.31 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
32,891 Da
OMIM
phenotype 615403
3D Structure
ModBase 3D Structure for Q86W42
Target Species
Human
Immunogen Description
WDR58 (NP_077315, 71 a.a. ~ 181 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
THOC6 antibody was raised against wDR58 (NP_077315, 71 a.a. ~ 181 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-THOC6 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-THOC6 antibody
ELISA, IHC-P (5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control PC-12. Western Blot (Cell lysate) - positive control A-431. Sandwich ELISA (Recombinant protein).

Immunohistochemistry (IHC) of anti-THOC6 antibody

Anti-THOC6 / WDR58 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-THOC6 Monoclonal Antibody – Cat# MBS246017

Immunohistochemistry (IHC) of anti-THOC6 antibody

Anti-THOC6 / WDR58 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-THOC6 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246017

Western Blot (WB) of anti-THOC6 antibody

WDR58 monoclonal antibody, clone 1F6 Western blot of WDR58 expression in A-431.
Mouse Anti-THOC6 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246017

Western Blot (WB) of anti-THOC6 antibody

WDR58 monoclonal antibody, clone 1F6. Western blot of WDR58 expression in PC-12.
Mouse Anti-THOC6 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246017

ELISA (EIA) of anti-THOC6 antibody

Detection limit for recombinant GST tagged WDR58 is approximately 0.3 ng/ml as a capture antibody.
Mouse Anti-THOC6 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246017

Other Notes

Small volumes of anti-THOC6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for THOC6. It may not necessarily be applicable to this product.

NCBI GI #
31543164
NCBI GeneID
79228
NCBI Accession #
NP_077315.2
UniProt Primary Accession #
Q86W42
UniProt Secondary Accession #
Q8NBR1; Q9BTV9; B2RA85
UniProt Related Accession #
Q86W42
GenBank Nucleotide #
NM_024339.3
NCBI Official Full Name
THO complex subunit 6 homolog isoform 1
NCBI Official Synonym Full Names
THO complex 6 homolog (Drosophila)
NCBI Official Symbol
THOC6
NCBI Official Synonym Symbols
BBIS; WDR58; fSAP35
UniProt Protein Name
THO complex subunit 6 homolog
UniProt Synonym Protein Names
Functional spliceosome-associated protein 35; fSAP35; WD repeat-containing protein 58
Protein Family
THO complex
UniProt Gene Name
THOC6
UniProt Synonym Gene Names
WDR58; fSAP35
UniProt Entry Name
THOC6_HUMAN
NCBI Protein Information
THO complex subunit 6 homolog; WD repeat domain 58; WD repeat-containing protein 58; functional spliceosome-associated protein 35

UniProt Comments for THOC6

THOC6: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. Belongs to the WD repeat THOC6 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: RNA binding

Biological Process: mRNA export from nucleus; central nervous system development; apoptosis; RNA splicing; intronless viral mRNA export from host nucleus; mRNA processing; negative regulation of apoptosis

Disease: Beaulieu-boycott-innes Syndrome
Show more

Research Articles on THOC6

1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-THOC6 antibody

 Reference Product  PubMed Publications
 THOC4 antibody  >1 publications with THOC6 and THOC4
 THOC7 antibody  >1 publications with THOC6 and THOC7
 THOC5 antibody  >1 publications with THOC6 and THOC5
 THOC1 antibody  >1 publications with THOC6 and THOC1
 THOC2 antibody  >1 publications with THOC6 and THOC2

Pathways associated with anti-THOC6 antibody

 Products by Pathway  Pathway Diagram
 RNA Transport Pathway antibodies  RNA Transport Pathway Diagram
 RNA Transport Pathway antibodies  RNA Transport Pathway Diagram
 THC Complex Pathway antibodies  THC Complex Pathway Diagram
 THC Complex Pathway antibodies  THC Complex Pathway Diagram
 TREX Complex Pathway antibodies  TREX Complex Pathway Diagram
 TREX Complex Pathway antibodies  TREX Complex Pathway Diagram
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