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SPRY1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246050

MBS246050 is an antibody targeting SPRY1. Also known as Mouse Monoclonal [clone 3H4] (IgG2a,k) to Human SPRY1 / Sprouty 1. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
SPRY1 / Sprouty 1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 3H4] (IgG2a,k) to Human SPRY1 / Sprouty 1
Product Gene Name
anti-SPRY1 antibody
Product Synonym Names
Anti-SPRY1 / Sprouty 1 Antibody (clone 3H4) IHC-plus; SPRY1; HSPRY1; Protein sprouty homolog 1; Spry-1; Sprouty 1; Human SPRY1; Sprouty 1
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3H4
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4.
Purity/Purification
Protein A Purified
Concentration
0.49 mg/ml (lot specific)
Specificity
Human SPRY1
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
35,122 Da
OMIM
602465
3D Structure
ModBase 3D Structure for O43609
Target Species
Human
Immunogen Description
SPRY1 (NP_005832, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
SPRY1 / Sprouty 1 antibody was raised against sPRY1 (NP_005832, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-SPRY1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SPRY1 antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-SPRY1 antibody

Anti-SPRY1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-SPRY1 Monoclonal Antibody – Cat# MBS246050

Western Blot (WB) of anti-SPRY1 antibody

Western blot of SPRY1 expression in transfected 293T cell line by SPRY1 monoclonal antibody, clone 3H4.
Mouse Anti-SPRY1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246050

Western Blot (WB) of anti-SPRY1 antibody

SPRY1 monoclonal antibody, clone 3H4. Western blot of SPRY1 expression in HepG2.
Mouse Anti-SPRY1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246050

ELISA (EIA) of anti-SPRY1 antibody

Detection limit for recombinant GST tagged SPRY1 is approximately 0.03 ng/ml as a capture antibody.
Mouse Anti-SPRY1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246050

Other Notes

Small volumes of anti-SPRY1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPRY1. It may not necessarily be applicable to this product.

NCBI GI #
384475544
NCBI GeneID
10252
NCBI Accession #
NP_001244967.1
UniProt Primary Accession #
O43609
UniProt Secondary Accession #
Q6PNE0; D3DNX6
UniProt Related Accession #
O43609
GenBank Nucleotide #
NM_001258038.1
NCBI Official Full Name
protein sprouty homolog 1
NCBI Official Synonym Full Names
sprouty homolog 1, antagonist of FGF signaling (Drosophila)
NCBI Official Symbol
SPRY1
NCBI Official Synonym Symbols
hSPRY1
UniProt Protein Name
Protein sprouty homolog 1
Protein Family
Protein sprouty
UniProt Gene Name
SPRY1
UniProt Synonym Gene Names
Spry-1
UniProt Entry Name
SPY1_HUMAN
NCBI Protein Information
protein sprouty homolog 1; spry-1; sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)

UniProt Comments for SPRY1

SPRY1: May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Belongs to the sprouty family. Induced by FGF signaling.

Protein type: Motility/polarity/chemotaxis; Inhibitor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q28.1

Cellular Component: plasma membrane; cytosol

Molecular Function: protein binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of cell proliferation; negative regulation of MAP kinase activity; establishment of mitotic spindle orientation; negative regulation of fibroblast growth factor receptor signaling pathway; ureteric bud development; negative regulation of nerve growth factor receptor signaling pathway; induction of an organ; negative regulation of Ras protein signal transduction; metanephros development
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Research Articles on SPRY1

1. Sprouty1 expression is not connected with mitogenic signaling and cell proliferation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPRY1 antibody

 Reference Product  PubMed Publications
 SPRY2 antibody  >49 publications with SPRY1 and SPRY2
 GRB2 antibody  >7 publications with SPRY1 and GRB2
 CBL antibody  >7 publications with SPRY1 and CBL
 EGFR antibody  >1 publications with SPRY1 and EGFR

Pathways associated with anti-SPRY1 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 EGFR Downregulation Pathway antibodies  EGFR Downregulation Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By EGFR Pathway antibodies  Signaling By EGFR Pathway Diagram
 Signaling By EGFR In Cancer Pathway antibodies  Signaling By EGFR In Cancer Pathway Diagram
 Signaling By EGFRvIII In Cancer Pathway antibodies  Signaling By EGFRvIII In Cancer Pathway Diagram
 Signaling By Ligand-Responsive EGFR Variants In Cancer Pathway antibodies  Signaling By Ligand-Responsive EGFR Variants In Cancer Pathway Diagram

Diseases associated with anti-SPRY1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >11 publications with SPRY1 and Cardiovascular Diseases
 Skin Diseases Antibodies  >10 publications with SPRY1 and Skin Diseases
 Breast Neoplasms Antibodies  >9 publications with SPRY1 and Breast Neoplasms
 Hypertrophy Antibodies  >8 publications with SPRY1 and Hypertrophy
 Fibrosis Antibodies  >8 publications with SPRY1 and Fibrosis
 Adenocarcinoma Antibodies  >7 publications with SPRY1 and Adenocarcinoma
 Congenital Abnormalities Antibodies  >7 publications with SPRY1 and Congenital Abnormalities
 Prostatic Neoplasms Antibodies  >6 publications with SPRY1 and Prostatic Neoplasms
 Disease Models, Animal Antibodies  >6 publications with SPRY1 and Disease Models, Animal
 Liver Diseases Antibodies  >5 publications with SPRY1 and Liver Diseases

Organs/Tissues associated with anti-SPRY1 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >25 publications with SPRY1 and Nerve
 Embryonic Tissue Antibodies  >25 publications with SPRY1 and Embryonic Tissue
 Kidney Antibodies  >19 publications with SPRY1 and Kidney
 Muscle Antibodies  >18 publications with SPRY1 and Muscle
 Blood Antibodies  >16 publications with SPRY1 and Blood
 Heart Antibodies  >15 publications with SPRY1 and Heart
 Brain Antibodies  >15 publications with SPRY1 and Brain
 Bone Antibodies  >12 publications with SPRY1 and Bone
 Vascular Antibodies  >10 publications with SPRY1 and Vascular
 Prostate Antibodies  >8 publications with SPRY1 and Prostate
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