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PDE9A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246067

Phosphodiesterase 9A is a cGMP-specific phosphodiesterase. PDE9A activity is inhibited by zaprinast, but it is insensitive to rolipram, vinpocetine, SKF-94120, dipyridamole, and IBMX. More than 20 alternatively spliced variants have been reported (Rentero et… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
PDE9A, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1E1] (IgG2a,k) to Human PDE9A
Product Gene Name
Product Synonym Names
Anti-PDE9A Antibody (clone 1E1) IHC-plus; PDE9A; Pde9A1; Pde9a2; Pde9a3; Pde9a4; Phosphodiesterase 9A; Phosphodiesterase PDE9A21; HSPDE9A2; Human PDE9A
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1E1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human PDE9A
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
56,554 Da
OMIM
602973
3D Structure
ModBase 3D Structure for O76083
Target Species
Human
Immunogen Description
Recombinant protein
Immunogen Type
Recombinant protein
Applications Tested/Suitable for anti-PDE9A antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PDE9A antibody
ELISA, IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot with the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-PDE9A antibody

Anti-PDE9A antibody IHC of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-PDE9A Monoclonal Antibody – Cat# MBS246067

Immunohistochemistry (IHC) of anti-PDE9A antibody

Anti-PDE9A antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-PDE9A Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246067

ELISA (EIA) of anti-PDE9A antibody

Detection limit for recombinant GST tagged PDE9A is 1 ng/ml as a capture antibody.
Mouse Anti-PDE9A Monoclonal Antibody, ELISA (EIA) – Cat# MBS246067

Other Notes

Small volumes of anti-PDE9A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDE9A purchase

MBS246067 is an antibody targeting PDE9A. Also known as Mouse Monoclonal [clone 1E1] (IgG2a,k) to Human PDE9A. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE9A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform a
NCBI Official Synonym Full Names
phosphodiesterase 9A
NCBI Official Symbol
PDE9A
NCBI Official Synonym Symbols
HSPDE9A2
UniProt Protein Name
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
UniProt Gene Name
PDE9A
UniProt Entry Name
PDE9A_HUMAN
NCBI Protein Information
high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A; phosphodiesterase PDE9A21; CGMP-specific 3',5'-cyclic phosphodiesterase type 9

NCBI Summary for PDE9A

The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for PDE9A

PDE9A: Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily. 16 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; EC 3.1.4.35; Phosphodiesterase

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; endoplasmic reticulum; perikaryon; cytosol

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; protein binding; metal ion binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: cGMP-mediated signaling; metabolic process; signal transduction; blood coagulation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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