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STAG2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246117

MBS246117 is an antibody targeting STAG2. Also known as Mouse Monoclonal [clone 3C6] (IgG1,k) to Human STAG2. Reactive in Human, Mouse. Host: Mouse. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
STAG2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 3C6] (IgG1,k) to Human STAG2
Product Gene Name
anti-STAG2 antibody
Product Synonym Names
Anti-STAG2 Antibody (clone 3C6) IHC-plus; STAG2; Cohesin subunit SA-2; SCC3B; SCC3 homolog 2; Stromal antigen 2; SA2; BA517O1.1; SA-2; Human STAG2
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3C6
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
PBS, pH 7.4.
Purity/Purification
Purified from ascites by Protein A
Concentration
0.5 mg/ml (lot specific)
Specificity
Human Nuclear Protein SA-2
Preparation and Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
OMIM
300826
3D Structure
ModBase 3D Structure for Q8N3U4
Immunogen Species
STAG2 antibody was raised against Human
Antigen type
Recombinant Protein
Target
STAG2
Immunogen
STAG2 antibody was raised against sTAG2 (NP_006594, 1130 a.a. ~ 1232 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-STAG2 antibody
Western Blot (WB), ELISA (EIA),Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF)
Application Notes for anti-STAG2 antibody
Western Blot (WB)
ELISA (optimal dilution to be determined by the researcher)
IHC-P (5 ug/ml)
Immunofluorescence (10ug/ml)

Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Cell lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-STAG2 antibody

Anti-STAG2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-STAG2 Monoclonal Antibody – Cat# MBS246117

Immunofluorescence (IF) of anti-STAG2 antibody

Immunofluorescence of monoclonal antibody to STAG2 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-STAG2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS246117

Western Blot (WB) of anti-STAG2 antibody

Western blot of STAG2 expression in NIH/3T3 cells with STAG2 monoclonal antibody, clone 3C6.
Mouse Anti-STAG2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246117

Western Blot (WB) of anti-STAG2 antibody

Western blot of STAG2 expression in HeLa nuclear extract with STAG2 monoclonal antibody, clone 3C6.
Mouse Anti-STAG2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246117

Other Notes

Small volumes of anti-STAG2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for STAG2. It may not necessarily be applicable to this product.

NCBI GI #
31563531
NCBI GeneID
10735
NCBI Accession #
NP_006594.3
UniProt Primary Accession #
Q8N3U4
UniProt Secondary Accession #
O00540; Q5JTI6; Q68DE9; Q9H1N8; B1AMT5; D3DTF5
UniProt Related Accession #
Q8N3U4
GenBank Nucleotide #
NM_006603.4
NCBI Official Full Name
cohesin subunit SA-2 isoform b
NCBI Official Synonym Full Names
stromal antigen 2
NCBI Official Symbol
STAG2
NCBI Official Synonym Symbols
SA2; SA-2; SCC3B; bA517O1.1
UniProt Protein Name
Cohesin subunit SA-2
UniProt Synonym Protein Names
SCC3 homolog 2; Stromal antigen 2
Protein Family
Cofilin
UniProt Gene Name
STAG2
UniProt Synonym Gene Names
SA2
UniProt Entry Name
STAG2_HUMAN
NCBI Protein Information
cohesin subunit SA-2; SCC3 homolog 2

NCBI Summary for STAG2

The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
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UniProt Comments for STAG2

STAG2: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3. Belongs to the SCC3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; membrane; plasma membrane; nucleolus; chromosome; chromatin; nucleus; cytosol; chromosome, pericentric region; actin cytoskeleton

Molecular Function: protein binding; chromatin binding

Biological Process: mitosis; meiotic cell cycle; cell division; stem cell maintenance; sister chromatid cohesion; negative regulation of DNA endoreduplication; mitotic cell cycle
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Research Articles on STAG2

1. These data suggest that PARP is a potential target for tumors harboring inactivating mutations in STAG2, and strongly recommend that STAG2 status be determined and correlated with therapeutic response to PARP inhibitors

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-STAG2 antibody

 Reference Product  PubMed Publications
 SMC3 antibody  >11 publications with STAG2 and SMC3
 RAD21 antibody  >10 publications with STAG2 and RAD21
 SMC1A antibody  >5 publications with STAG2 and SMC1A
 ESPL1 antibody  >2 publications with STAG2 and ESPL1
 SMC1B antibody  >1 publications with STAG2 and SMC1B
 PLK1 antibody  >1 publications with STAG2 and PLK1

Pathways associated with anti-STAG2 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cohesin Loading Onto Chromatin Pathway antibodies  Cohesin Loading Onto Chromatin Pathway Diagram
 Establishment Of Sister Chromatid Cohesion Pathway antibodies  Establishment Of Sister Chromatid Cohesion Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Meiosis Pathway antibodies  Meiosis Pathway Diagram
 Meiotic Synapsis Pathway antibodies  Meiotic Synapsis Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram

Diseases associated with anti-STAG2 antibody

 Disease Name  Pubmed Publications
 Leukemia, Myeloid Antibodies  >7 publications with STAG2 and Leukemia, Myeloid
 Urinary Bladder Neoplasms Antibodies  >6 publications with STAG2 and Urinary Bladder Neoplasms
 Carcinoma, Transitional Cell Antibodies  >4 publications with STAG2 and Carcinoma, Transitional Cell
 Lung Neoplasms Antibodies  >2 publications with STAG2 and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with STAG2 and Cell Transformation, Neoplastic
 Learning Disorders Antibodies  >1 publications with STAG2 and Learning Disorders
 Congenital Abnormalities Antibodies  >1 publications with STAG2 and Congenital Abnormalities
 Kidney Diseases Antibodies  >1 publications with STAG2 and Kidney Diseases
 Precancerous Conditions Antibodies  >1 publications with STAG2 and Precancerous Conditions
 Necrosis Antibodies  >1 publications with STAG2 and Necrosis

Organs/Tissues associated with anti-STAG2 antibody

 Organ/Tissue Name  Pubmed Publications
 Bladder Antibodies  >6 publications with STAG2 and Bladder
 Blood Antibodies  >5 publications with STAG2 and Blood
 Bone Antibodies  >3 publications with STAG2 and Bone
 Stomach Antibodies  >2 publications with STAG2 and Stomach
 Lung Antibodies  >2 publications with STAG2 and Lung
 Ovary Antibodies  >1 publications with STAG2 and Ovary
 Mouth Antibodies  >1 publications with STAG2 and Mouth
 Prostate Antibodies  >1 publications with STAG2 and Prostate
 Skin Antibodies  >1 publications with STAG2 and Skin
 Muscle Antibodies  >1 publications with STAG2 and Muscle
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