Mouse Anti-TIMP2 Monoclonal Antibody – Cat# MBS4380255

Formalin-fixed, paraffin-embedded human Placenta stained with TIMP2 Monoclonal Antibody (3A4).

TIMP2 Antibody – Mouse Monoclonal

Name: TIMP2 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS4380255
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380255

MBS4380255 is an antibody targeting TIMP2. Also known as TIMP2 (Tissue Inhibitor of Metalloproteinase 2). Reactive in Human, Mouse, Rat, Guinea pig, Rabbit, Cow, Zebrafish. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TIMP2 (Tissue Inhibitor of Metalloproteinase 2), Monoclonal Antibody

Full Product Name

TIMP2 (Tissue Inhibitor of Metalloproteinase 2)

Product Gene Name

anti-TIMP2 antibody

Product Synonym Names

Metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP-2; TIMP2; Tissue Inhibitor of Metalloproteinase 2

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone Number

3A4

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Guinea pig, Rabbit, Cow, Zebrafish. Others not known.

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

21kDa

3D Structure

ModBase 3D Structure for P16035

Immunogen

A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Cellular Localization

Cytoplasm and Cell Surface

Hu-chromosome Location

17q25

Positive Control

HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Applications Tested/Suitable for anti-TIMP2 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

Application Notes for anti-TIMP2 antibody

FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Product References and Citations for anti-TIMP2 antibody

Murray et al. 1998. Gut. 43(6):791-7. Jeffery et al. 2009. Histopathology. 54(7):820-8

Other Notes

Small volumes of anti-TIMP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TIMP2. It may not necessarily be applicable to this product.

NCBI GI #

4507511

NCBI GeneID

7077

NCBI Accession #

NP_003246.1

UniProt Primary Accession #

P16035

UniProt Secondary Accession #

Q16121; Q93006; Q9UDF7

UniProt Related Accession #

P16035

GenBank Nucleotide #

NM_003255.4

NCBI Official Full Name

metalloproteinase inhibitor 2

NCBI Official Synonym Full Names

TIMP metallopeptidase inhibitor 2

NCBI Official Symbol

TIMP2

NCBI Official Synonym Symbols

DDC8; CSC-21K

UniProt Protein Name

Metalloproteinase inhibitor 2

UniProt Synonym Protein Names

CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2

Protein Family

Metalloproteinase inhibitor

UniProt Gene Name

TIMP2

UniProt Synonym Gene Names

TIMP-2

NCBI Protein Information

metalloproteinase inhibitor 2

NCBI Summary for TIMP2

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]

UniProt Comments for TIMP2

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

Research Articles on TIMP2

1. Urine levels of TIMP2 (and IGFBP7) are predictive of acute kidney injury following cardiac surgical procedures.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TIMP2 antibody

Reference Product	PubMed Publications
MMP2 antibody	>222 publications with TIMP2 and MMP2
MMP14 antibody	>26 publications with TIMP2 and MMP14
MMP8 antibody	>5 publications with TIMP2 and MMP8
ITGB1 antibody	>1 publications with TIMP2 and ITGB1

Pathways associated with anti-TIMP2 antibody

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Angiogenesis Pathway antibodies	Angiogenesis Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Matrix Metalloproteinases Pathway antibodies	Matrix Metalloproteinases Pathway Diagram

Diseases associated with anti-TIMP2 antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>56 publications with TIMP2 and Cardiovascular Diseases
Fibrosis Antibodies	>40 publications with TIMP2 and Fibrosis
Neoplasm Metastasis Antibodies	>38 publications with TIMP2 and Neoplasm Metastasis
Inflammation Antibodies	>36 publications with TIMP2 and Inflammation
Disease Models, Animal Antibodies	>31 publications with TIMP2 and Disease Models, Animal
Heart Diseases Antibodies	>30 publications with TIMP2 and Heart Diseases
Nervous System Diseases Antibodies	>21 publications with TIMP2 and Nervous System Diseases
Liver Diseases Antibodies	>20 publications with TIMP2 and Liver Diseases
Necrosis Antibodies	>20 publications with TIMP2 and Necrosis
Lung Neoplasms Antibodies	>15 publications with TIMP2 and Lung Neoplasms

Organs/Tissues associated with anti-TIMP2 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>76 publications with TIMP2 and Blood
Vascular Antibodies	>63 publications with TIMP2 and Vascular
Embryonic Tissue Antibodies	>38 publications with TIMP2 and Embryonic Tissue
Bone Antibodies	>33 publications with TIMP2 and Bone
Muscle Antibodies	>31 publications with TIMP2 and Muscle
Liver Antibodies	>31 publications with TIMP2 and Liver
Heart Antibodies	>29 publications with TIMP2 and Heart
Lung Antibodies	>29 publications with TIMP2 and Lung
Kidney Antibodies	>27 publications with TIMP2 and Kidney
Uterus Antibodies	>20 publications with TIMP2 and Uterus