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HCN1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246184

MBS246184 is an antibody targeting HCN1. Also known as Mouse Monoclonal [clone S70-28] (IgG1) to Rat HCN1. Reactive in Human, Mouse, Rat. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
HCN1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone S70-28] (IgG1) to Rat HCN1
Product Gene Name
anti-HCN1 antibody
Product Synonym Names
Anti-HCN1 Antibody (aa778-910, clone S70-28) IHC-plus; HCN1; BCNG1; BCNG-1; HAC2; HAC-2; Rat HCN1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S70-28
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, pH 7.4, 50% glycerol, 0.09% sodium azide
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~100 kD protein. No cross reactivity against HCN2.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
98,796 Da
OMIM
gene 615871
3D Structure
ModBase 3D Structure for O60741
Target Species
Rat
Immunogen Description
Fusion protein amino acids 778-910 (C terminus) of rat HCN1, accession number Q9JKB0.
Immunogen
HCN1 antibody was raised against fusion protein amino acids 778-910 (C terminus) of rat HCN1, accession number Q9JKB0.
Antigen Modification
aa778-910

Product Categories/Family for anti-HCN1 antibody

Family: Ion Channel; Subfamily: Potassium channel - cyclic nucleotide gated
Applications Tested/Suitable for anti-HCN1 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-HCN1 antibody
ICC (0.1 - 1 ug/ml), IHC-P (1:50), IP (1 - 10 ug/ml), WB (1 - 10 ug/ml)
Usage: Western Blot: Use the lysate in SDS buffer but without boiling, incubating for 20 mins at 37 degree C instead.

Immunohistochemistry (IHC) of anti-HCN1 antibody

Anti-HCN1 antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Mouse Anti-HCN1 Monoclonal Antibody – Cat# MBS246184

Immunohistochemistry (IHC) of anti-HCN1 antibody

IHC analysis of HCN1 in frozen sections of mouse brain extract using HCN1 antibody.
Mouse Anti-HCN1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246184

Immunofluorescence (IF) of anti-HCN1 antibody

HCN1 (S70-28), Human Hippocampus.
Mouse Anti-HCN1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS246184

Western Blot (WB) of anti-HCN1 antibody

Western blot analysis of HCN1 in rat brain membrane lysates using a 1:1000 dilution of HCN1 antibody.
Mouse Anti-HCN1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246184

Other Notes

Small volumes of anti-HCN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HCN1. It may not necessarily be applicable to this product.

NCBI GI #
116325989
NCBI GeneID
348980
NCBI Accession #
NP_066550.2
UniProt Primary Accession #
O60741
UniProt Related Accession #
O60741
GenBank Nucleotide #
NM_021072.3
NCBI Official Full Name
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
NCBI Official Synonym Full Names
hyperpolarization activated cyclic nucleotide-gated potassium channel 1
NCBI Official Symbol
HCN1
NCBI Official Synonym Symbols
BCNG1; HAC-2; BCNG-1; EIEE24
UniProt Protein Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
UniProt Synonym Protein Names
Brain cyclic nucleotide-gated channel 1; BCNG-1
Protein Family
Anaphase-promoting complex
UniProt Gene Name
HCN1
UniProt Synonym Gene Names
BCNG1; BCNG-1
UniProt Entry Name
HCN1_HUMAN
NCBI Protein Information
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1; brain cyclic nucleotide gated channel 1; brain cyclic nucleotide-gated channel 1

NCBI Summary for HCN1

The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes. [provided by RefSeq, Oct 2011]
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UniProt Comments for HCN1

HCN1: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. Belongs to the potassium channel HCN family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: integral to plasma membrane; axon; dendrite; plasma membrane; integral to membrane

Molecular Function: identical protein binding; voltage-gated potassium channel activity; potassium channel activity; intracellular cAMP activated cation channel activity; voltage-gated sodium channel activity; cAMP binding

Biological Process: synaptic transmission; apical protein localization; regulation of membrane potential; retinal cone cell development; potassium ion transport

Disease: Epileptic Encephalopathy, Early Infantile, 24
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Research Articles on HCN1

1. de novo HCN1 point mutations cause a recognizable early-onset epileptic encephalopathy in humans

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HCN1 antibody

 Reference Product  PubMed Publications
 HCN2 antibody  >194 publications with HCN1 and HCN2
 HCN4 antibody  >134 publications with HCN1 and HCN4
 HCN3 antibody  >51 publications with HCN1 and HCN3
 GNAT3 antibody  >1 publications with HCN1 and GNAT3

Pathways associated with anti-HCN1 antibody

 Products by Pathway  Pathway Diagram
 HCN Channels Pathway antibodies  HCN Channels Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Potassium Channels Pathway antibodies  Potassium Channels Pathway Diagram

Diseases associated with anti-HCN1 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >28 publications with HCN1 and Disease Models, Animal
 Neurologic Manifestations Antibodies  >24 publications with HCN1 and Neurologic Manifestations
 Inflammation Antibodies  >5 publications with HCN1 and Inflammation
 Tachycardia Antibodies  >2 publications with HCN1 and Tachycardia
 Skin Diseases Antibodies  >2 publications with HCN1 and Skin Diseases
 Ataxia Antibodies  >2 publications with HCN1 and Ataxia
 Basal Ganglia Diseases Antibodies  >1 publications with HCN1 and Basal Ganglia Diseases
 Necrosis Antibodies  >1 publications with HCN1 and Necrosis

Organs/Tissues associated with anti-HCN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >197 publications with HCN1 and Brain
 Muscle Antibodies  >87 publications with HCN1 and Muscle
 Eye Antibodies  >21 publications with HCN1 and Eye
 Embryonic Tissue Antibodies  >12 publications with HCN1 and Embryonic Tissue
 Uncharacterized Tissue Antibodies  >2 publications with HCN1 and Uncharacterized Tissue
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