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NEFH Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246190

MBS246190 is an antibody targeting NEFH. Also known as Mouse Monoclonal [clone RNF405] (IgG2a) to Human NEFH / NF-H. Reactive in Bovine, Chicken, Dog, Xenopus, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
NEFH / NF-H, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone RNF405] (IgG2a) to Human NEFH / NF-H
Product Gene Name
anti-NEFH antibody
Product Synonym Gene Name
NF-H
Product Synonym Names
Anti-NEFH / NF-H Antibody (clone RNF405) IHC-plus; NEFH; 200 kDa neurofilament protein; KIAA0845; NFH; NF-H; Human NEFH; NF-H
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
RNF405
Host
Mouse
Species Reactivity
Bovine, Chicken, Dog, Xenopus, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep
Form/Format
PBS containing 0.09% sodium azide
Purity/Purification
Purified
Concentration
1 mg/ml (lot specific)
Specificity
Reacts exclusively with the phosphorylated isoform of the 200 kD neurofilament protein.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
105,639 Da
OMIM
phenotype 162230
3D Structure
ModBase 3D Structure for P12036
Target Species
Human
Immunogen Description
Neurofilament preparation of calf brain tissue
Applications Tested/Suitable for anti-NEFH antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB)
Application Notes for anti-NEFH antibody
IHC-Fr (1:50 - 1:100), IHC-P (10 ug/ml), WB (1:100 - 1:500)

Immunohistochemistry (IHC) of anti-NEFH antibody

Anti-NEFH antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-NEFH Monoclonal Antibody – Cat# MBS246190

Immunohistochemistry (IHC) of anti-NEFH antibody

Immunofluorescence staining of a 9 days old zebrafish embryo
Mouse Anti-NEFH Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246190

Other Notes

Small volumes of anti-NEFH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEFH. It may not necessarily be applicable to this product.

NCBI GI #
32483416
NCBI GeneID
4744
NCBI Accession #
NP_066554.2
UniProt Primary Accession #
P12036
UniProt Secondary Accession #
Q96HF8; Q9UJS7; Q9UQ14; B4DYY4
UniProt Related Accession #
P12036
GenBank Nucleotide #
NM_021076.3
NCBI Official Full Name
neurofilament heavy polypeptide
NCBI Official Synonym Full Names
neurofilament, heavy polypeptide
NCBI Official Symbol
NEFH
NCBI Official Synonym Symbols
NFH
UniProt Protein Name
Neurofilament heavy polypeptide
UniProt Synonym Protein Names
200 kDa neurofilament protein; Neurofilament triplet H protein
Protein Family
Neurofilament heavy polypeptide
UniProt Gene Name
NEFH
UniProt Synonym Gene Names
KIAA0845; NFH; NF-H
UniProt Entry Name
NFH_HUMAN
NCBI Protein Information
neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa

NCBI Summary for NEFH

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
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UniProt Comments for NEFH

NFH: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: mitochondrion; axon; cytoplasm; neurofilament

Molecular Function: protein binding, bridging; dynein binding; structural constituent of cytoskeleton; kinesin binding; microtubule binding; structural molecule activity; protein kinase binding

Biological Process: neurofilament bundle assembly; axonogenesis; cell projection biogenesis; microtubule cytoskeleton organization and biogenesis; peripheral nervous system neuron axonogenesis

Disease: Amyotrophic Lateral Sclerosis 1
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Research Articles on NEFH

1. pNFL-H may be useful in determining which individuals require CT imaging to assess the severity of their injury

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NEFH antibody

 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
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