• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ENO3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246210

MBS246210 is an antibody targeting ENO3. Also known as Mouse Monoclonal [clone 5D1] (IgG2a,k) to Human ENO3 / Enolase 3. Reactive in Human. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
ENO3 / Enolase 3, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 5D1] (IgG2a,k) to Human ENO3 / Enolase 3
Product Gene Name
anti-ENO3 antibody
Product Synonym Names
Anti-ENO3 / Enolase 3 Antibody (clone 5D1) IHC-plus; ENO3; Beta-enolase; Enolase 3; Enolase 3 (beta; muscle); Enolase 3; (beta; muscle); Muscle-specific enolase; MSE; Skeletal muscle enolase; GSD13; Human ENO3; Enolase 3
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
5D1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human ENO3
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
42,248 Da
OMIM
gene 612932
3D Structure
ModBase 3D Structure for P13929
Target Species
Human
Immunogen Description
ENO3 (NP_001967, 228 a.a. ~ 278 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
ENO3 / Enolase 3 antibody was raised against eNO3 (NP_001967, 228 a.a. ~ 278 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-ENO3 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ENO3 antibody
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-ENO3 antibody

Anti-ENO3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-ENO3 Monoclonal Antibody – Cat# MBS246210

Immunofluorescence (IF) of anti-ENO3 antibody

Immunofluorescence of monoclonal antibody to ENO3 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-ENO3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS246210

Western Blot (WB) of anti-ENO3 antibody

Western blot of ENO3 expression in transfected 293T cell line by ENO3 monoclonal antibody (M01), clone 5D1.
Mouse Anti-ENO3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246210

Western Blot (WB) of anti-ENO3 antibody

ENO3 monoclonal antibody (M01), clone 5D1 Western blot of ENO3 expression in HeLa.
Mouse Anti-ENO3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246210

RNA interference of anti-ENO3 antibody

Western blot of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody, clone 5D1. GAPDH (36.1 kD) used as specificity an.
Mouse Anti-ENO3 Monoclonal Antibody, RNA interference – Cat# MBS246210

ELISA (EIA) of anti-ENO3 antibody

Detection limit for recombinant GST tagged ENO3 is approximately 0.03 ng/ml as a capture antibody.
Mouse Anti-ENO3 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246210

Other Notes

Small volumes of anti-ENO3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ENO3. It may not necessarily be applicable to this product.

NCBI GI #
153267427
NCBI GeneID
2027
NCBI Accession #
NP_001967.2
UniProt Primary Accession #
P13929
UniProt Secondary Accession #
Q96AE2; B4DUI6; B4DUM6; D3DTL2; E7ENK8
UniProt Related Accession #
P13929
GenBank Nucleotide #
NM_001976.3
NCBI Official Full Name
beta-enolase
NCBI Official Synonym Full Names
enolase 3 (beta, muscle)
NCBI Official Symbol
ENO3
NCBI Official Synonym Symbols
MSE; GSD13
UniProt Protein Name
Beta-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; MSE; Skeletal muscle enolase
Protein Family
Beta-enolase
UniProt Gene Name
ENO3
UniProt Synonym Gene Names
MSE
UniProt Entry Name
ENOB_HUMAN
NCBI Protein Information
beta-enolase; muscle-specific enolase; skeletal muscle enolase; 2-phospho-D-glycerate hydrolyase

NCBI Summary for ENO3

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]
Show more

UniProt Comments for ENO3

ENO3: Appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen- beta particles are detected on ultrastructural analysis. Belongs to the enolase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; EC 4.2.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: extracellular space; phosphopyruvate hydratase complex; cytoplasm; plasma membrane; cytosol

Molecular Function: protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: response to drug; glycolysis; carbohydrate metabolic process; skeletal muscle regeneration; glucose metabolic process; pathogenesis; aging; gluconeogenesis

Disease: Glycogen Storage Disease Xiii
Show more

Research Articles on ENO3

1. Pyridoxamine and carnosine protected enolase against the total loss of catalytic activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ENO3 antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram

Diseases associated with anti-ENO3 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >3 publications with ENO3 and Liver Diseases
 Lung Diseases Antibodies  >2 publications with ENO3 and Lung Diseases
 Liver Neoplasms Antibodies  >2 publications with ENO3 and Liver Neoplasms
 Lung Neoplasms Antibodies  >2 publications with ENO3 and Lung Neoplasms
 Heart Diseases Antibodies  >1 publications with ENO3 and Heart Diseases
 Liver Neoplasms, Experimental Antibodies  >1 publications with ENO3 and Liver Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >1 publications with ENO3 and Carcinoma, Hepatocellular
 Cardiovascular Diseases Antibodies  >1 publications with ENO3 and Cardiovascular Diseases
 Weight Gain Antibodies  >1 publications with ENO3 and Weight Gain
 Weight Loss Antibodies  >1 publications with ENO3 and Weight Loss

Organs/Tissues associated with anti-ENO3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >11 publications with ENO3 and Muscle
 Liver Antibodies  >8 publications with ENO3 and Liver
 Heart Antibodies  >5 publications with ENO3 and Heart
 Brain Antibodies  >4 publications with ENO3 and Brain
 Lung Antibodies  >3 publications with ENO3 and Lung
 Blood Antibodies  >2 publications with ENO3 and Blood
 Kidney Antibodies  >2 publications with ENO3 and Kidney
 Placenta Antibodies  >1 publications with ENO3 and Placenta
 Testis Antibodies  >1 publications with ENO3 and Testis
 Intestine Antibodies  >1 publications with ENO3 and Intestine
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000