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GMEB1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246211

MBS246211 is an antibody targeting GMEB1. Also known as Mouse Monoclonal [clone 2A8] (IgG2a,k) to Human GMEB1. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
GMEB1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2A8] (IgG2a,k) to Human GMEB1
Product Gene Name
Product Synonym Names
Anti-GMEB1 Antibody (clone 2A8) IHC-plus; GMEB1; DNA-binding protein p96PIF; p96PIF; PIF p96; PIF96; GMEB-1; Human GMEB1
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2A8
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2
Purity/Purification
Protein A Purified
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
61,327 Da
OMIM
604409
3D Structure
ModBase 3D Structure for Q9Y692
Target Species
Human
Immunogen Description
GMEB1 (NP_077808, 467 a.a. ~ 564 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
GMEB1 antibody was raised against gMEB1 (NP_077808, 467 a.a. ~ 564 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-GMEB1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-GMEB1 antibody
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control HeLa NE. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections, 3 ug/ml) - positive control human colon. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombin...

Immunohistochemistry (IHC) of anti-GMEB1 antibody

Anti-GMEB1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-GMEB1 Monoclonal Antibody – Cat# MBS246211

Immunofluorescence (IF) of anti-GMEB1 antibody

Immunofluorescence of monoclonal antibody to GMEB1 on HeLa cell (antibody concentration 10 ug/ml).
Mouse Anti-GMEB1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS246211

Western Blot (WB) of anti-GMEB1 antibody

GMEB1 monoclonal antibody clone 2A8 Western blot of GMEB1 expression in HeLa NE.
Mouse Anti-GMEB1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246211

ELISA (EIA) of anti-GMEB1 antibody

Detection limit for recombinant GST tagged GMEB1 is approximately 0.03 ng/ml as a capture antibody.
Mouse Anti-GMEB1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246211

Other Notes

Small volumes of anti-GMEB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GMEB1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glucocorticoid modulatory element-binding protein 1 isoform 2
NCBI Official Synonym Full Names
glucocorticoid modulatory element binding protein 1
NCBI Official Symbol
GMEB1
NCBI Official Synonym Symbols
PIF96; P96PIF
UniProt Protein Name
Glucocorticoid modulatory element-binding protein 1
UniProt Synonym Protein Names
DNA-binding protein p96PIF; Parvovirus initiation factor p96; PIF p96
UniProt Gene Name
GMEB1
UniProt Synonym Gene Names
GMEB-1; PIF p96
UniProt Entry Name
GMEB1_HUMAN
NCBI Protein Information
glucocorticoid modulatory element-binding protein 1; GMEB-1; PIF p96; DNA-binding protein p96PIF; parvovirus initiation factor p96

NCBI Summary for GMEB1

This gene encodes a member of KDWK gene family which associates with GMEB2 protein. The GMEB1-GMEB2 complex is essential for parvovirus DNA replication. Studies in rat for a similar gene suggest that this gene's role is to modulate the transactivation of the glucocorticoid receptor when it is bound to glucocorticoid response elements. Two alternative spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Feb 2011]
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UniProt Comments for GMEB1

GMEB1: Trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. Binds also to the transferrin receptor promoter. Essential auxiliary factor for the replication of parvoviruses. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding; transcription coactivator activity; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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