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HSP70 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246248

MBS246248 is an antibody targeting HSP70. Also known as Mouse Monoclonal [clone 3A3] (IgG1) to Human HSP70 / Heat Shock Protein 70. Reactive in Amphibian, Chicken, Drosophila, Fish, Human, Mouse, Rat, Yeast. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
HSP70 / Heat Shock Protein 70, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 3A3] (IgG1) to Human HSP70 / Heat Shock Protein 70
Product Gene Name
Product Synonym Names
Anti-HSP70 / Heat Shock Protein 70 Antibody (clone 3A3) IHC-plus; Heat Shock Protein 70; Human HSP70; Heat Shock Protein 70
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3A3
Host
Mouse
Species Reactivity
Amphibian, Chicken, Drosophila, Fish, Human, Mouse, Rat, Yeast
Form/Format
PBS, pH 7.2; 50% glycerol, 0.09% azide. Sourced in Ascites.
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects several members of the heat shock protein 70kD gene family including Hsp70, Hsc70, p75 and following heat shock, Hsp72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples.
Preparation and Storage
Store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
70,295 Da
Target Species
Human
Immunogen Description
Human Recombinant Hsp70 overexpressed in E Coli.
Immunogen Type
Recombinant protein
Immunogen
Human Recombinant Hsp70 overexpressed in E Coli.
Applications Tested/Suitable for anti-HSP70 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-HSP70 antibody
ICC (1:500), IHC-P (2.5 ug/ml), IP, WB (1:5000)
Usage: Rat lysate used is a mix of protein lysates from skeletal muscle, brain, heart, kidney, lung, pancreas, spleen, testes and thymus.

Immunohistochemistry (IHC) of anti-HSP70 antibody

Anti-HSP70 / Heat Shock Protein 70 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Mouse Anti-HSP70 Monoclonal Antibody – Cat# MBS246248

Western Blot (WB) of anti-HSP70 antibody

Hsp70 (3A3), Artemia franciscanna.
Mouse Anti-HSP70 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246248

Western Blot (WB) of anti-HSP70 antibody

Hsp70 (3A3), Rat tissue lysate.
Mouse Anti-HSP70 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246248

Other Notes

Small volumes of anti-HSP70 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSP70. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Official Full Name
heat shock protein 70
NCBI Official Symbol
HSPA1A
NCBI Official Synonym Symbols
HSP70
UniProt Protein Name
Heat shock protein 70
UniProt Synonym Protein Names
Heat shock protein 70
UniProt Gene Name
HSP70
UniProt Entry Name
H2DGR2_SHEEP
NCBI Protein Information
heat shock 70 kDa protein 1A/1B

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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