• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Cytokeratin Type II (KRT75) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246262

MBS246262 is an antibody targeting KRT75. Also known as Mouse Monoclonal [clone AE3] (IgG1) to Human Cytokeratin Type II. Reactive in Human. Host: Mouse. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
Cytokeratin Type II (KRT75), Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone AE3] (IgG1) to Human Cytokeratin Type II
Product Gene Name
Product Synonym Names
Anti-Cytokeratin Type II Antibody (clone AE3) IHC-plus; Cytokeratin Type II; Human Cytokeratin Type II
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AE3
Host
Mouse
Species Reactivity
Human
Form/Format
0.01M PBS containing 0.1% Sodium azide and 1% BSA. Sourced in Ascites.
Purity/Purification
Purified IgG
Concentration
1 mg/ml (lot specific)
Specificity
This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.
Preparation and Storage
Store at 4 degree C.
Molecular Weight
59,560 Da
OMIM
gene 612318
3D Structure
ModBase 3D Structure for O95678
Target Species
Human
Immunogen Description
Human epidermal cells.
Immunogen Type
Cells
Immunogen
Human epidermal cells.
Applications Tested/Suitable for anti-KRT75 antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB)
Application Notes for anti-KRT75 antibody
IHC-Fr, IHC-P (1:100), WB (1:100)
Usage: In unfixed frozen sections and formalin-fixed paraffin embedded tissues. Also applicable in Western blotting. For use on formalin-fixed paraffin embedded tissues a dilution of approximately 1:150 to 1:200 is appropriate if section are incubated for 3...

Immunohistochemistry (IHC) of anti-KRT75 antibody

Anti-Cytokeratin Type II antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Mouse Anti-KRT75 Monoclonal Antibody – Cat# MBS246262

Immunohistochemistry (IHC) of anti-KRT75 antibody

Anti-Cytokeratin Type II antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Mouse Anti-KRT75 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246262

Other Notes

Small volumes of anti-KRT75 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT75. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
cytokeratin type II
UniProt Protein Name
Keratin, type II cytoskeletal 75
UniProt Synonym Protein Names
Cytokeratin-75; CK-75; Keratin-6 hair follicle; hK6hf; Keratin-75; K75; Type II keratin-K6hf; Type-II keratin Kb18
Protein Family
UniProt Gene Name
KRT75
UniProt Synonym Gene Names
K6HF; KB18; CK-75; hK6hf; K75
UniProt Entry Name
K2C75_HUMAN

UniProt Comments for KRT75

KRT75: Plays a central role in hair and nail formation. Essential component of keratin intermediate filaments in the companion layer of the hair follicle. Defects in KRT75 may be a cause of loose anagen hair syndrome (LAHS). In LAHS, anagen hairs are easily pulled from the scalp. The hair is relatively sparse and does not grow long. Hair of fair color and hair shafts of reduced caliber, and an early age of onset are features. Usually the hairs are not fragile and there are no areas of breakage. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: keratin filament

Molecular Function: structural molecule activity

Biological Process: hemopoietic progenitor cell differentiation

Disease: Pseudofolliculitis Barbae
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000