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ISLET-1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246283

ISL1 encodes islet 1, a transcription factor containing two amino-terminal LIM domains and one carboxy-terminal homeodomain. ISL1 plays an important role in the embryogenesis of pancreatic islets of Langerhans. In addition, mouse embryos made deficient in… More details

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Product Overview

Online Datasheet in PDF
Product Name
ISLET-1 / ISL1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1H9] (IgG1) to Human ISLET-1 / ISL1
Product Gene Name
anti-ISLET-1 antibody
Product Synonym Gene Name
ISL1
Product Synonym Names
Anti-ISLET-1 / ISL1 Antibody (clone 1H9) IHC-plus; ISL1; Isl-1; ISLET1; ISL LIM homeobox 1; Islet-1; Human ISLET-1; ISL1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1H9
Host
Mouse
Species Reactivity
Human
Form/Format
Ascites fluid, 0.03% sodium azide. Sourced in Ascites.
Purity/Purification
Ascites
Specificity
Human ISL1
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
Molecular Weight
39,036 Da
OMIM
600366
3D Structure
ModBase 3D Structure for P61371
Target Species
Human
Immunogen Description
Purified recombinant fragment of human ISL1 expressed in E Coli.
Immunogen
ISLET-1 / ISL1 antibody was raised against purified recombinant fragment of human ISL1 expressed in E Coli.
Applications Tested/Suitable for anti-ISLET-1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ISLET-1 antibody
ELISA (1:10000), IF (1:200 - 1:1000), IHC-P (1:200), WB (1:500 - 1:2000)

Immunohistochemistry (IHC) of anti-ISLET-1 antibody

Anti-ISL1 / Islet 1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Mouse Anti-ISLET-1 Monoclonal Antibody – Cat# MBS246283

Immunohistochemistry (IHC) of anti-ISLET-1 antibody

Anti-ISL1 / Islet 1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Mouse Anti-ISLET-1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246283

Western Blot (WB) of anti-ISLET-1 antibody

IHC of paraffin-embedded human lung cancer (left) and cervical carcinoma (right), showing nuclear localization using anti-ISL1 antibody with DAB staining.
Mouse Anti-ISLET-1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246283

Other Notes

Small volumes of anti-ISLET-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ISLET-1 purchase

MBS246283 is an antibody targeting ISLET-1. Also known as Mouse Monoclonal [clone 1H9] (IgG1) to Human ISLET-1 / ISL1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ISLET-1. It may not necessarily be applicable to this product.

NCBI GI #
115387114
NCBI GeneID
3670
NCBI Accession #
NP_002193.2
UniProt Primary Accession #
P61371
UniProt Secondary Accession #
P20663; P47894
UniProt Related Accession #
P61371
GenBank Nucleotide #
NM_002202.2
NCBI Official Full Name
insulin gene enhancer protein ISL-1
NCBI Official Synonym Full Names
ISL LIM homeobox 1
NCBI Official Symbol
ISL1
NCBI Official Synonym Symbols
Isl-1; ISLET1
UniProt Protein Name
Insulin gene enhancer protein ISL-1
UniProt Gene Name
ISL1
UniProt Synonym Gene Names
Islet-1
UniProt Entry Name
ISL1_HUMAN
NCBI Protein Information
insulin gene enhancer protein ISL-1; islet-1; ISL1 transcription factor, LIM/homeodomain

NCBI Summary for ISLET-1

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]
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UniProt Comments for ISLET-1

ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Cell development/differentiation; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; zinc ion binding; bHLH transcription factor binding; estrogen receptor binding; chromatin binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-12 production; positive regulation of interleukin-1 alpha production; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of estrogen receptor signaling pathway; pancreas development; positive regulation of cell proliferation; neuron fate specification; ventricular cardiac muscle morphogenesis; negative regulation of neuron apoptosis; sensory system development; neural crest cell migration; positive regulation of DNA binding; trigeminal nerve development; pharyngeal system development; axon regeneration; spinal cord motor neuron cell fate specification; positive regulation of insulin secretion; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; spinal cord motor neuron differentiation; positive regulation of interleukin-1 beta production; positive regulation of angiogenesis; negative regulation of neuron differentiation; positive regulation of interferon-gamma production; mesenchymal cell differentiation; visceral motor neuron differentiation; pituitary gland development; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; peripheral nervous system neuron axonogenesis; retinal ganglion cell axon guidance
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Research Articles on ISLET-1

1. ISL1 is commonly expressed in rhabdomyosarcoma, particularly the alveolar subtype

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ISLET-1 antibody

 Reference Product  PubMed Publications
 MEF2C antibody  >17 publications with ISLET-1 and MEF2C
 SHH antibody  >15 publications with ISLET-1 and SHH
 BMP4 antibody  >12 publications with ISLET-1 and BMP4
 FGF10 antibody  >10 publications with ISLET-1 and FGF10
 INS antibody  >8 publications with ISLET-1 and INS

Pathways associated with anti-ISLET-1 antibody

 Products by Pathway  Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Heart Development Pathway antibodies  Heart Development Pathway Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway antibodies  Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway Diagram

Diseases associated with anti-ISLET-1 antibody

 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >36 publications with ISLET-1 and Heart Diseases
 Cardiovascular Diseases Antibodies  >35 publications with ISLET-1 and Cardiovascular Diseases
 Congenital Abnormalities Antibodies  >31 publications with ISLET-1 and Congenital Abnormalities
 Heart Defects, Congenital Antibodies  >24 publications with ISLET-1 and Heart Defects, Congenital
 Cardiovascular Abnormalities Antibodies  >22 publications with ISLET-1 and Cardiovascular Abnormalities
 Nervous System Diseases Antibodies  >10 publications with ISLET-1 and Nervous System Diseases
 Lung Neoplasms Antibodies  >5 publications with ISLET-1 and Lung Neoplasms
 Fibrosis Antibodies  >4 publications with ISLET-1 and Fibrosis
 Neoplasm Metastasis Antibodies  >4 publications with ISLET-1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >4 publications with ISLET-1 and Breast Neoplasms

Organs/Tissues associated with anti-ISLET-1 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >120 publications with ISLET-1 and Embryonic Tissue
 Muscle Antibodies  >101 publications with ISLET-1 and Muscle
 Pancreas Antibodies  >62 publications with ISLET-1 and Pancreas
 Brain Antibodies  >53 publications with ISLET-1 and Brain
 Eye Antibodies  >43 publications with ISLET-1 and Eye
 Lung Antibodies  >11 publications with ISLET-1 and Lung
 Stomach Antibodies  >5 publications with ISLET-1 and Stomach
 Intestine Antibodies  >3 publications with ISLET-1 and Intestine
 Kidney Antibodies  >3 publications with ISLET-1 and Kidney
 Umbilical Cord Antibodies  >2 publications with ISLET-1 and Umbilical Cord
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