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DUX4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246334

MBS246334 is an antibody targeting DUX4. Also known as Mouse Monoclonal [clone P2B1] (IgG1) to Human DUX4. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DUX4, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone P2B1] (IgG1) to Human DUX4
Product Gene Name
anti-DUX4 antibody
Product Synonym Names
Anti-DUX4 Antibody (clone P2B1) IHC-plus; DUX4; Double homeobox protein 10; Double homeobox protein DUX10; Double homeobox 4; Double homeobox protein 4/10; Double homeobox protein 4; DUX10; Human DUX4
Clonality
Monoclonal
Isotype
IgG1
Clone Number
P2B1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS pH 7.4, in 0.09% azide in 50% glycerol
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
This antibody recognizeshuman DUX4. It does notcross-react with DUX4c.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
44,940 Da
OMIM
phenotype 606009
3D Structure
ModBase 3D Structure for Q9UBX2
Target Species
Human
Immunogen Description
C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag
Immunogen Type
Fusion protein
Immunogen
DUX4 antibody was raised against c-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag
Applications Tested/Suitable for anti-DUX4 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-DUX4 antibody
IF, IHC-P (10 ug/ml), WB (1:1000)

Immunohistochemistry (IHC) of anti-DUX4 antibody

Anti-DUX4 antibody IHC of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Mouse Anti-DUX4 Monoclonal Antibody – Cat# MBS246334

Immunofluorescence (IF) of anti-DUX4 antibody

Immunofluorescent staining using P2B1 mouse monoclonal anti-DUX4 N-terminus on C2C12 myoblasts transfected with pCS2+DUX4. Counter-stained with DAPI for nuclei.
Mouse Anti-DUX4 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS246334

Western Blot (WB) of anti-DUX4 antibody

Western blot using P2B1 anti-DUX4 N-terminus mouse monoclonal on C2C12 cells transfected with pCS2+DUX4 which contains an additional upstream start site.
Mouse Anti-DUX4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246334

Other Notes

Small volumes of anti-DUX4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DUX4. It may not necessarily be applicable to this product.

NCBI GI #
194272172
NCBI GeneID
22947
NCBI Accession #
NP_149418.3
UniProt Primary Accession #
Q9UBX2
GenBank Nucleotide #
NM_033178.2
NCBI Official Full Name
double homeobox protein 4
NCBI Official Synonym Full Names
double homeobox 4 like 1
NCBI Official Symbol
DUX4L1
NCBI Official Synonym Symbols
DUX4; DUX10
UniProt Protein Name
Double homeobox protein 4
UniProt Synonym Protein Names
Double homeobox protein 10
Protein Family
Double homeobox protein
UniProt Gene Name
DUX4
UniProt Entry Name
DUX4_HUMAN

NCBI Summary for DUX4

This gene is located within a D4Z4 repeat array in the subtelomeric region of chromosome 4q. The D4Z4 repeat is polymorphic in length and a similar D4Z4 repeat array has been identified on chromosome 10. Each D4Z4 repeat unit has an open reading frame (named DUX4) that encodes two homeoboxes; the repeat-array and ORF is conserved in other mammals. There is no evidence for transcription of the gene at this locus though RT-PCR and in vitro expression experiments indicate that a telomeric paralog of this gene is transcribed in some haplotypes. Contraction of the macrosatellite repeat causes autosomal dominant facioscapulohumeral muscular dystrophy (FSHD). [provided by RefSeq, Jun 2014]
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UniProt Comments for DUX4

DUX4: May be involved in transcriptional regulation. Defects in DUX4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Severity is highly variable. Weakness is slowly progressive and about 20% of affected individuals eventually require a wheelchair. Approximately 70-90% of individuals have inherited the disease-causing deletion from a parent, and approximately 10-30% of affected individuals have FSHD as the result of a de novo deletion. Offsprings of an affected individual have a 50% chance of inheriting the deletion. Belongs to the paired homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 4q35
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Research Articles on DUX4

1. these data support a model in which DME1 and DME2 myogenic enhancers associate with the DUX4 promoter in skeletal myocytes and activate transcription when epigenetically derepressed in FSHD, resulting in the pathological misexpression of DUX4-fl.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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