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HCLS1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246336

MBS246336 is an antibody targeting HCLS1. Also known as Mouse Monoclonal [clone 2A8] (IgG1,k) to Human HCLS1. Reactive in Human. Host: Mouse. More details

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Product Overview

Product Name
HCLS1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2A8] (IgG1,k) to Human HCLS1
Product Gene Name
anti-HCLS1 antibody
Product Synonym Names
Anti-HCLS1 Antibody (clone 2A8) IHC-plus; HCLS1; CTTNL; LckBP1; p75; HS1; Human HCLS1
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2A8
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
24,008 Da
OMIM
601306
3D Structure
ModBase 3D Structure for P14317
Target Species
Human
Immunogen Description
HCLS1 (AAH16758, 266 a.a. ~ 356 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
HCLS1 antibody was raised against hCLS1 (AAH16758, 266 a.a. ~ 356 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-HCLS1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-HCLS1 antibody
ELISA, IHC-P (5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control K-562. ELISA (Recombinant protein).

Immunohistochemistry (IHC) of anti-HCLS1 antibody

Anti-HCLS1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-HCLS1 Monoclonal Antibody – Cat# MBS246336

Immunohistochemistry (IHC) of anti-HCLS1 antibody

Anti-HCLS1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-HCLS1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246336

Immunohistochemistry (IHC) of anti-HCLS1 antibody

Anti-HCLS1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-HCLS1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246336

Western Blot (WB) of anti-HCLS1 antibody

HCLS1 monoclonal antibody, clone 2A8 Western blot of HCLS1 expression in K-562.
Mouse Anti-HCLS1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246336

ELISA (EIA) of anti-HCLS1 antibody

Detection limit for recombinant GST tagged HCLS1 is approximately 0.3 ng/ml as a capture antibody.
Mouse Anti-HCLS1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246336

Other Notes

Small volumes of anti-HCLS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HCLS1. It may not necessarily be applicable to this product.

NCBI GI #
167234422
NCBI GeneID
3059
NCBI Accession #
NP_005326.2
UniProt Primary Accession #
P14317
UniProt Secondary Accession #
Q53Y93; Q6IBK9; Q9UDK0; B4DQ69
UniProt Related Accession #
P14317
GenBank Nucleotide #
NM_005335.5
NCBI Official Full Name
hematopoietic lineage cell-specific protein isoform 1
NCBI Official Synonym Full Names
hematopoietic cell-specific Lyn substrate 1
NCBI Official Symbol
HCLS1
NCBI Official Synonym Symbols
HS1; p75; CTTNL; lckBP1
UniProt Protein Name
Hematopoietic lineage cell-specific protein
UniProt Synonym Protein Names
Hematopoietic cell-specific LYN substrate 1; LckBP1; p75
Protein Family
Hematopoietic lineage cell-specific protein
UniProt Gene Name
HCLS1
UniProt Synonym Gene Names
HS1
UniProt Entry Name
HCLS1_HUMAN
NCBI Protein Information
hematopoietic lineage cell-specific protein

UniProt Comments for HCLS1

HS1: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS. Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54. Expressed only in tissues and cells of hematopoietic origin.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: transcription factor complex; mitochondrion; membrane; cytoplasm; nucleus

Molecular Function: protein binding; protein complex binding; actin binding; SH3 domain binding; protein kinase binding

Biological Process: positive regulation of granulocyte differentiation; response to hormone stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; regulation of actin filament polymerization; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of STAT protein; regulation of transcription, DNA-dependent; actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of macrophage differentiation
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Research Articles on HCLS1

1. Phosphoproteome analyses reveal specific implications of Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a myeloid progenitor cell line downstream of wild-type and ITD mutant Fms-like tyrosine kinase 3 receptors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HCLS1 antibody

 Reference Product  PubMed Publications
 SRC antibody  >33 publications with HCLS1 and SRC
 LYN antibody  >26 publications with HCLS1 and LYN
 SYK antibody  >10 publications with HCLS1 and SYK

Pathways associated with anti-HCLS1 antibody

 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies  Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
 IL-5 Signaling Pathway antibodies  IL-5 Signaling Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram

Diseases associated with anti-HCLS1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3 publications with HCLS1 and Nervous System Diseases
 Inflammation Antibodies  >2 publications with HCLS1 and Inflammation
 Glioblastoma Antibodies  >1 publications with HCLS1 and Glioblastoma
 Lung Neoplasms Antibodies  >1 publications with HCLS1 and Lung Neoplasms
 Ovarian Neoplasms Antibodies  >1 publications with HCLS1 and Ovarian Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with HCLS1 and Neoplasms, Experimental
 Learning Disorders Antibodies  >1 publications with HCLS1 and Learning Disorders
 Abnormalities, Drug-Induced Antibodies  >1 publications with HCLS1 and Abnormalities, Drug-Induced
 Necrosis Antibodies  >1 publications with HCLS1 and Necrosis
 Glomerulonephritis, IGA Antibodies  >1 publications with HCLS1 and Glomerulonephritis, IGA

Organs/Tissues associated with anti-HCLS1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >72 publications with HCLS1 and Blood
 Nerve Antibodies  >4 publications with HCLS1 and Nerve
 Bone Antibodies  >4 publications with HCLS1 and Bone
 Liver Antibodies  >4 publications with HCLS1 and Liver
 Bone Marrow Antibodies  >4 publications with HCLS1 and Bone Marrow
 Spleen Antibodies  >3 publications with HCLS1 and Spleen
 Lymph Node Antibodies  >2 publications with HCLS1 and Lymph Node
 Muscle Antibodies  >2 publications with HCLS1 and Muscle
 Lymph Antibodies  >2 publications with HCLS1 and Lymph
 Heart Antibodies  >1 publications with HCLS1 and Heart
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