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RHOA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246347

MBS246347 is an antibody targeting RHOA. Also known as Mouse Monoclonal [clone 1B12] (IgG1,l) to Human RHOA. Reactive in Human. Host: Mouse. More details

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Product Overview

Product Name
RHOA, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1B12] (IgG1,l) to Human RHOA
Product Gene Name
anti-RHOA antibody
Product Synonym Names
Anti-RHOA Antibody (clone 1B12) IHC-plus; RHOA; ARH12; ARHA; Aplysia ras-related homolog 12; H12; Oncogene RHO H12; Rho cDNA clone 12; RHO12; RHOH12; Ras homolog family member A; Small GTP binding protein RhoA; Transforming protein RhoA; Human RHOA
Clonality
Monoclonal
Isotype
IgG1,l
Clone Number
1B12
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human RHOA
Preparation and Storage
Aliquot and store at -20 degree C or -80 degree C. Avoid freeze-thaw cycles.
Molecular Weight
21,768 Da
OMIM
165390
3D Structure
ModBase 3D Structure for P61586
Target Species
Human
Immunogen Description
RHOA (AAH01360, 1 a.a. ~ 194 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
RHOA antibody was raised against rHOA (AAH01360, 1 a.a. ~ 194 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Product Categories/Family for anti-RHOA antibody

Family: Ras GTPase superfamily IPR001806
Applications Tested/Suitable for anti-RHOA antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RHOA antibody
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-RHOA antibody

Anti-RHOA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-RHOA Monoclonal Antibody – Cat# MBS246347

Immunohistochemistry (IHC) of anti-RHOA antibody

Anti-RHOA antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-RHOA Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246347

Immunofluorescence (IF) of anti-RHOA antibody

Immunofluorescence of monoclonal antibody to RHOA on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-RHOA Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS246347

Western Blot (WB) of anti-RHOA antibody

Western blot of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.
Mouse Anti-RHOA Monoclonal Antibody, Western Blot (WB) – Cat# MBS246347

Western Blot (WB) of anti-RHOA antibody

Western blot of RHOA expression in HL-60 cell lysate.
Mouse Anti-RHOA Monoclonal Antibody, Western Blot (WB) – Cat# MBS246347

ELISA (EIA) of anti-RHOA antibody

Detection limit for recombinant GST tagged RHOA is approximately 10 ng/ml as a capture antibody.
Mouse Anti-RHOA Monoclonal Antibody, ELISA (EIA) – Cat# MBS246347

Other Notes

Small volumes of anti-RHOA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RHOA. It may not necessarily be applicable to this product.

NCBI GI #
10835049
NCBI GeneID
387
NCBI Accession #
NP_001655.1
UniProt Primary Accession #
P61586
UniProt Secondary Accession #
P06749; Q53HM4; Q5U024; Q9UDJ0; Q9UEJ4
UniProt Related Accession #
P61586
GenBank Nucleotide #
NM_001664.2
NCBI Official Full Name
transforming protein RhoA
NCBI Official Synonym Full Names
ras homolog family member A
NCBI Official Symbol
RHOA
NCBI Official Synonym Symbols
ARHA; ARH12; RHO12; RHOH12
UniProt Protein Name
Transforming protein RhoA
UniProt Synonym Protein Names
Rho cDNA clone 12; h12
Protein Family
GTP-binding protein
UniProt Gene Name
RHOA
UniProt Synonym Gene Names
ARH12; ARHA; RHO12; h12
UniProt Entry Name
RHOA_HUMAN
NCBI Protein Information
transforming protein RhoA; h12; oncogene RHO H12; rho cDNA clone 12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; ras homolog gene family, member A

UniProt Comments for RHOA

RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: Motility/polarity/chemotaxis; G protein; Oncoprotein; G protein, monomeric; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: apical junction complex; focal adhesion; cytoskeleton; lamellipodium; plasma membrane; cell cortex; midbody; cell junction; cytosol; cleavage furrow

Molecular Function: GTPase activity; protein binding; myosin binding; GTP binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; negative chemotaxis; positive regulation of NF-kappaB import into nucleus; regulation of axonogenesis; regulation of cell migration; Rho protein signal transduction; regulation of osteoblast proliferation; substantia nigra development; small GTPase mediated signal transduction; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of axonogenesis; positive regulation of cytokinesis; platelet activation; apical junction assembly; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; regulation of small GTPase mediated signal transduction; positive regulation of axonogenesis; blood coagulation; actin cytoskeleton organization and biogenesis; positive regulation of neuron differentiation; vascular endothelial growth factor receptor signaling pathway
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Research Articles on RHOA

1. Inactivation of RhoA due to lack of prenylation leads to Rac1 activation and subsequent priming for IL-1beta secretion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RHOA antibody

 Reference Product  PubMed Publications
 ROCK1 antibody  >158 publications with RHOA and ROCK1
 ARHGDIA antibody  >44 publications with RHOA and ARHGDIA
 ARHGEF12 antibody  >42 publications with RHOA and ARHGEF12
 SMURF1 antibody  >36 publications with RHOA and SMURF1
 RTKN antibody  >22 publications with RHOA and RTKN
 ARHGEF11 antibody  >18 publications with RHOA and ARHGEF11
 PKN1 antibody  >3 publications with RHOA and PKN1
 UBC antibody  >1 publications with RHOA and UBC
 ARHGAP1 antibody  >1 publications with RHOA and ARHGAP1

Pathways associated with anti-RHOA antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Arf6 Downstream Pathway antibodies  Arf6 Downstream Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram

Diseases associated with anti-RHOA antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >502 publications with RHOA and Cardiovascular Diseases
 Carcinoma Antibodies  >380 publications with RHOA and Carcinoma
 Nervous System Diseases Antibodies  >351 publications with RHOA and Nervous System Diseases
 Disease Models, Animal Antibodies  >350 publications with RHOA and Disease Models, Animal
 Hypertension Antibodies  >332 publications with RHOA and Hypertension
 Neoplasm Invasiveness Antibodies  >329 publications with RHOA and Neoplasm Invasiveness
 Inflammation Antibodies  >314 publications with RHOA and Inflammation
 Neoplasm Metastasis Antibodies  >253 publications with RHOA and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >195 publications with RHOA and Breast Neoplasms
 Heart Diseases Antibodies  >180 publications with RHOA and Heart Diseases

Organs/Tissues associated with anti-RHOA antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >1249 publications with RHOA and Vascular
 Muscle Antibodies  >1241 publications with RHOA and Muscle
 Blood Antibodies  >1031 publications with RHOA and Blood
 Lung Antibodies  >471 publications with RHOA and Lung
 Nerve Antibodies  >459 publications with RHOA and Nerve
 Heart Antibodies  >398 publications with RHOA and Heart
 Kidney Antibodies  >355 publications with RHOA and Kidney
 Liver Antibodies  >247 publications with RHOA and Liver
 Bone Antibodies  >244 publications with RHOA and Bone
 Embryonic Tissue Antibodies  >227 publications with RHOA and Embryonic Tissue
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